Labshake search
Citations for Merck :
101 - 150 of 642 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... After testing for SARS-CoV-2 by PCR test (COBAS 6800, Merck México, Mexico city), they were classified as positive (AP ...
-
bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3)pLysS competent cells (Novagen/Merck, Darmstadt, Germany). An overnight culture grown in dYT medium supplemented with 100 μg/ml carbenicillin and 34 μg/ml chloramphenicol was diluted to an OD600 of 0.1 with dYT supplemented with 100 μg/ml carbenicillin and grown at 37°C and 200 rpm to an OD600 of 0.6 ...
-
bioRxiv - Cell Biology 2020Quote: Escherichia coli Rosetta (DE3) pLysS Competent Cells (Merck Cat# 70956) containing pBAD-thio-topo-LmaCK1.2-V5-His6 ...
-
bioRxiv - Neuroscience 2023Quote: ... Lipopolysaccharide from Escherichia coli (LPS; 026:B6; catalogue # L8274 Merck Life Sciences UK ...
-
bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag with the 3C protease (MERCK), the protein was again subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
-
bioRxiv - Physiology 2024Quote: ... sections were stained with hematoxylin and eosin (H&E; Merck, Zug, Switzerland).
-
bioRxiv - Evolutionary Biology 2020Quote: ... coli experiments were performed in LB (Merck 110285, Kenilworth, New Jersey) with the appropriate antibiotics and experiments with S ...
-
bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) Codon plus-RIL cells (Novagen, Merck, Darmstadt, Germany). PDI proteins were purified to homogeneity by nickel affinity chromatography as described by Wang et al ...
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
-
bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
-
bioRxiv - Biochemistry 2020Quote: Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) was transformed with pET28a-His6-CrTRXz and grown in 1 L of 2YT medium supplemented with kanamycin (50 µg.mL-1 ...
-
bioRxiv - Microbiology 2020Quote: ... coli onto a fresh plate of complex solid media (Merck LB 110283) containing selective antibiotics when appropriate ...
-
bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
-
bioRxiv - Biochemistry 2022Quote: AniA was heterologously produced in Escherichia coli BL21(DE3) competent cells (Merck). The expression vector encoding the soluble domain of AniA (pET28A_aniA ...
-
bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... coli by biochemical assays using Fluorocult LMX broth (Merck KGaA, Darmstadt, Germany) and API 20E strips (bioMerieux Inc. ...
-
bioRxiv - Biochemistry 2022Quote: ... Canada) and transformed in Escherichia coli BL21 (DE3) (Novagen/Merck, Darmstadt, Germany) for protein production ...
-
bioRxiv - Microbiology 2023Quote: ... coli) on filters with a pore size of 0.22 μm (Merck Millipore) for 24 hours at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... coli ESBL058 was inoculated into LB media (Lysogeny Broth, Merck, Darmstadt, Germany) and incubated until exponential phase at 37 °C at 180 rpm ...
-
bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Paraffin sections (5 μm) were stained with hematoxylin/eosin (H&E; Merck, Darmstadt, Germany). For immunohistochemistry (IHC) ...
-
bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
-
bioRxiv - Neuroscience 2022Quote: ... slides were counterstained with 0.1% eosin for 30 s (H&E, Merck, Darmstadt, Germany), followed by dehydrating steps of an ethanol series (96% ...
-
bioRxiv - Molecular Biology 2022Quote: ... Column chromatography was carried out using silica gel 60 (230-400 mesh, E. Merck) with the solvent system indicated in the procedures ...
-
bioRxiv - Immunology 2021Quote: Mice received a single dose of LPS 0111:B4 from Escheria coli (Merck) at 1mg/kg in sterile PBS ...
-
bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
-
bioRxiv - Pathology 2023Quote: ... The resulting plasmid was transformed in Escherichia Coli BL21 (DE3) comnpetent cells (MERCK). Expression of TAT-Cre was induced growing transformed cells in LB Overnight Express™ Instant medium (Novagen ...
-
bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
-
Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... General histology was assessed after tissue fixation with 4% paraformaldehyde by H&E staining (Merck) or Picro Sirius Red stain (Direct Red 80 (Sigma ...
-
bioRxiv - Developmental Biology 2023Quote: ... live imaging and tissue harvesting were performed under anaesthesia in 0.015% benzocaine (Merck E-1501) diluted in Vienna tap water ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-pro-surfactant-protein-C (rabbit, 1:20,000, Merck, AB3786, pSP-C), anti-rabbit IgG (goat ...
-
bioRxiv - Cell Biology 2023Quote: ... Mitomycin C (Merck (Roche), 10107409001) ...
-
bioRxiv - Biochemistry 2021Quote: Transformation of Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) were made with a pET3d-His6-CrFBA3 plasmid ...
-
bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK) followed by a gel filtration step (Superdex-200 10/300 GE Healthcare Life Sciences) ...
-
bioRxiv - Immunology 2019Quote: All E/S supernatant was collected and filter sterilised through a 0.2 μm syringe filter (Merck). E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore ...
-
bioRxiv - Physiology 2020Quote: ... minced ex situ and mixed with 30 ml of HBSS containing 0.07% Pronase E (Merck EMD), 50 mM HEPES and 0.6 mg/ml CaCl2*2H2O ...
-
bioRxiv - Physiology 2021Quote: ... For histological analysis TA sections were stained with hematoxylin and eosin (H&E; Merck, Zug, Switzerland). To determinate fiber type and fiber size sections were blocked and permeabilized in PBS containing 10% goat serum and 0.4% triton X-100 for 30 min before being incubated for 2 h at RT in a primary antibody solution containing BA-D5 ...
-
bioRxiv - Pathology 2023Quote: ... all experimental solutions contained 10 μM EMD-57033 (gift from Dr Norbert Beier, E. Merck Pharmaceuticals).
-
bioRxiv - Cancer Biology 2023Quote: ... TLC was performed on silica gel 60 F254 pre-coated TLC aluminum sheets (E. Merck, Germany), and two alternative reagents were used for visualization ...
-
bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... coli NovaBlue competent cells using the Novagen pETBlue-1 Perfectly Blunt Cloning Kit (Merck KGaA). The E ...
-
bioRxiv - Neuroscience 2020Quote: ... Antibodies against adenomatous polyposis coli (1:250; APC or CC1, Merck Millipore, Billerica, MA, USA) or GFP (1:8000 ...
-
bioRxiv - Cell Biology 2022Quote: All proteins used in this study were overexpressed in Rosetta (DE3, pLysS) Escherichia coli (MERCK) cells using 0.4 mM isopropyl 1-thio-D-galactopyranoside (Inalco Pharmaceuticals ...
-
bioRxiv - Microbiology 2019Quote: ... Salmonella enterica LT2 and Escherichia coli ATCC 25922 were grown anaerobically in LB (Merck – 110285); Lactobacillus bulgaricus LMG 6901 ...
-
bioRxiv - Biochemistry 2020Quote: ... calphostin C (208725, Merck-Millipore), tempol (176141 ...
-
bioRxiv - Biochemistry 2020Quote: ... compound C (171260, Merck-Millipore), calphostin C (208725 ...
-
bioRxiv - Neuroscience 2022Quote: FluoroJade C (FJC, Merck Millipore) staining was used to monitor cell death 2 and 4 days after ihKA injection ...
-
bioRxiv - Developmental Biology 2021Quote: ... plates or coverslips were coated with 100 μg/ml poly-D-lysine (Merck Millipore, A-003-E) at 37°C overnight ...
-
bioRxiv - Developmental Biology 2021Quote: ... the skin sections were stained with hematoxylin and eosin (H&E) and mounted with Entellan® (Merck). The stained slides were imaged using a DM2000 light microscope (Leica biosystems ...