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Citations for Merck :
201 - 250 of 1371 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 5 µg dephosphorylated myelin basic protein (Merck, 13-110), and purified MAK protein ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was concentrated using Amicon filters (Merck, Darmstadt, Germany) in buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cisplatin-induced DNA damage was assayed using an antibody against the DNA double strand break marker gamma-H2AX (Merck-Millipore) on tumor sections co-stained with DAPI to enumerate cell nuclei ...
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bioRxiv - Genetics 2022Quote: ... Transient transfection was carried out in HEK293T cells transfected with 1 μg of plasmid DNA in the presence of X-tremeGene9 DNA transfection reagent (#6365809001, Merck), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... whole plasmids (Entry plasmids containing cmk-1 coding DNA sequences) were amplified with the KOD Hot Start DNA Polymerase (Novagen, Merck). Primers were phosphorylated in 5’ and were designed to contain the desired point mutation(s ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Cell Biology 2021Quote: KOD-Xtreme hot-start DNA polymerase (Merck Millipore), DreamTaq™ Green PCR Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... KOD Hot Start DNA polymerase (0.02U/μL; Merck) was used with the following programme ...
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bioRxiv - Microbiology 2021Quote: ... using KOD Hot Start DNA polymerase (Merck Millipore) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... using the KOD Hot Start DNA polymerase (Merck). Amplification reactions performed with complementary oligonucleotides were then mixed ...
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bioRxiv - Genomics 2020Quote: ... DNA was stained using Hoechst 33342 (#B2261, Merck). Pictures were taken using Leica SP5 confocal microscope and Leica Application Suite software (Leica) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA transfection was performed with Genejuice (Merck Millipore) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... or KOD HotStart DNA polymerase (Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... KOD-Xtreme hot-start DNA polymerase (Merck Millipore), DreamTaq™ Green PCR Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: KOD-Xtreme hot-start DNA polymerase (Merck Millipore), DreamTaq™ Green PCR Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... or using KOD Hot-start DNA polymerase (Merck) followed by DpnI treatment ...
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bioRxiv - Microbiology 2022Quote: ... Peptide content in each fraction was verified using QuantiPro BCA Assay Kit (Merck). Zn content was determined using PAR against a standard curve.
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bioRxiv - Cell Biology 2022Quote: Wound healing assays were performed in Corning® 6-well plates (Merck, UK). HDF and MSC were individually labelled with cell tracking dyes eBioscience™ CFSE (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2019Quote: ... BR) and insulin Elisa assay (cat#EZRMI-13K, Merck Millipore, Billerica, MA, EUA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: SecA ATPase activity assay was done using malachite green kit (MAK307, Merck/Sigma). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell viability assays were carried out using WST-1 Cell Proliferation Reagent (Merck). A498 cells were plated onto clear 96-well plates at 4,000 cells per well and left to adhere for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... The PLA was performed using the Duolink Proximity Ligation Assay Kit Red (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was also assessed with MTT assay (Millipore, Merck Life Science, Germany). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability assay was performed by propidium iodide (PI) (P4864, Merck, Gillingham, UK) staining followed by flowcytometry analysis [29] ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were quenched using 100 μL 5 M H2SO4 (Merck, Rahway, United States). Ethylene and ethane were detected via gas chromatography using the gas chromatograph (GC ...
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bioRxiv - Cancer Biology 2024Quote: ... enhanced chemiluminescence assays were performed using the Millipore kit (Merck-Millipore, Stockholm, Sweden). The antibodies used are listed in the Supplementary Table S1 ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... after which Protein G agarose/Salmon sperm beads (Merck Millipore) were added and incubated for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... The protein was concentrated using a centrifugal filter (Merck Millipore) and the concentration was determined by UV absorbance using the corresponding ε values (M-1 cm-1).
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bioRxiv - Molecular Biology 2021Quote: Proteins were transferred onto Immobilon-P PVDF membranes (Merck IPVH00010) using a wet-transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merck) that were activated in methanol (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with a direct detection spectrometer (Merck), and purified proteins were stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were desalted using a PD-10 column (Merck), concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-myelin basic protein (MBP) (Polyclonal, Merck, Darmstadt, Germany); mouse anti-neurofilament-H protein (clone NE14 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); mouse anti-dephosphorylated neurofilament protein (clone smi32 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck, USA) using a wet transfer system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (as determined by Merck Direct Detect) were then separated by 4 to 12% bis-tris SDS-PAGE in NuPAGE MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-myelin basic protein (MBP) (Polyclonal, Merck, Darmstadt, Germany); mouse anti-neurofilament-H protein (clone NE14 ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was quantified using Ponceau S staining (Merck, UK) and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1 ...
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bioRxiv - Systems Biology 2020Quote: ... Separated proteins were transferred onto PVDF membranes (Merck Millipore, IPVH00010). Membranes were blocked in 5% BSA in PBST and antibodies diluted in 2% BSA in PBST ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were precipitated using five volumes of chilled acetone (Merck) at −20°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were then filtered through Miracloth (Merck, Germany) and subjected to a Co-IP analysis.