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Citations for Merck :
1 - 50 of 1371 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Neuroscience 2023Quote: ... eIF4E/eIF4G interaction inhibitor 4EGI-1 (40 µM; Merck 324517), mammalian Target of Rapamycin (200 nMmTOR ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Systems Biology 2019Quote: ... via a BCA protein assay (Merck-Millipore cat. 71285) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined via Bradford assay (Merck; #1.10306). Equal amounts of proteins (20–100μg as optimal for each antibody ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... a hydrophobic interaction chromatography (GE lifesciences, now Cytivia) and nano-filtration (Merck MilliPore, USA). These purified bulk mAbs were then mixed 1:1 (as per protein content ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of lysates were determined using BCA assay (Merck). Proteins in culture supernatants were precipitated using methanol/chloroform ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... and protein concentration was measured by Bradford assay (Merck, Gillingham, UK).
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... allowing the interaction to be observed as PLA foci within the cells (Adopted from Duolink PLA, Merck). PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were quantified using Direct Detect® Assay-free Cards (Merck; DDAC00010) or Pierce Bicinchoninic Bcid (BCA ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Microbiology 2021Quote: ... by using hydrophilic interaction ion chromatography with a ZIC cHILIC column (3.0 μm polymeric, 2.1×150mm; Sequant, Merck) as described previously [35] ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were then analysed by LC-MS by separating using hydrophilic interaction liquid chromatography with a SeQuant ZIC-pHILIC column (2.1 × 150 mm, 5 μm) (Merck). Analytes were detected with high-resolution ...
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bioRxiv - Biochemistry 2021Quote: Purified unlabelled DNA oligonucleotides used in NADH-coupled ATPase assays were purchased from Merck. Purified fluorescein-labelled DNA oligonucleotides used in EMSA experiments were purchased from Integrated DNA Technologies [Leuven ...
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bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Chromatin immunoprecipitation of sonicated DNA samples was carried out using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Merck KGaD) and anti-H3K4me3 (05-745R ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The rest of the supernatant for each sample is used to measure the total protein level of the sample with a Bradford assay (Merck # B6916), following reagent protocol ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP:ADP ratio assays were performed using an ADP/ATP ratio assay kit (Merck, MAK135).
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bioRxiv - Genetics 2023Quote: ... a glycerol assay kit (Merck), and a d-gluconic acid/d-glucono-δ-lactone assay kit (Megazyme International) ...
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bioRxiv - Cancer Biology 2023Quote: ... the MTT assay (Merck, 11465007001) was performed ...
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bioRxiv - Molecular Biology 2024Quote: ... the WST-1 assay (Merck) was used according to the manufacturer’s recommended protocol ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... For MTT assays (Millipore; Merck), MTT reagent was added for 4 h and absorbance measured as 570 nm (cell absorbance ...
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bioRxiv - Cancer Biology 2022Quote: Proximity ligation assay was performed with Duolink® Proximity Ligation Assay (Sigma Aldrich-Merck, DUO92102) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The chemotactic migration assay of THP1 was performed through the InnoCyte Monocyte Migration Assay (Merck Millipore). Cells were serum-starved for 3h prior to the experiment and ...
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bioRxiv - Cell Biology 2019Quote: MTT cell growth assay kit (Merck) was additionally employed to quantify cell viability upon LecB treatment ...
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bioRxiv - Cell Biology 2024Quote: LDH (lactate dehydrogenase) activity assay (Merck) is a colourimetric assay used to measure the concentration of LDH in the cell culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... The cell proliferation assay was done using the standard MTT assay (Sigma-Aldrich/Merck, Catalog No. 11465007001). MTT assay was performed on each well following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...