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Citations for Merck :
451 - 500 of 1371 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein detection was carried out with Immobilon Crescendo Western HRP substrate (Merck) using ChemiDoc™ MP System and images were analyzed using Image Lab™ Software (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... and the protein digests were washed twice with water saturated ethylacetate (Merck). Desalting of the proteolytic peptide sample was performed on a C18 solid phase extraction column (‘home-made ZipTip’ from a 15G punch from Empore™ octadecyl C18 47 mm extraction disks (3M ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were analysed by mPAGETM 4-20% Bis-Tris gels (Merck) and Coomassie staining ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Cell Biology 2023Quote: ... Protein levels were detected using chemiluminescent Clarity Western ECL reagent (Merck; GERPN2236) and developed using ImageQuant LAS 4000.
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aqueous solutions without protein components were filtered using Millex-GV filters (Merck), Novec HFE-7500 (3M ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Immunology 2021Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck) was used to measure IgG1 ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PLA assay was performed according to the manufacturer’s protocol (Duolink using PLA technology, Sigma-Aldrich, Merck) and as described before 43,44.
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bioRxiv - Pathology 2021Quote: ... The assay samples were read on a Luminex 200™ instrument with xPONENT software (Merck Millipore), as described by the manufacturer.
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bioRxiv - Microbiology 2021Quote: ... Firefly and Renilla luciferase activities were measured with a dual-luciferase reporter assay system from Merck Millipore as previously reported (Goueslain et al. ...
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bioRxiv - Cancer Biology 2022Quote: Cell migration was assessed using the QCM Chemotaxis Cell Migration Colorimetric Assay kit (ECM506, Merck, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Insulin was measured in EDTA plasma using a radio immuno assay (RIA, Merck Millipore, RI-13K). IAPP was measured by an enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Bioengineering 2020Quote: ... AGPAT1 was then amplified by PCR with KOD DNA polymerase (Merck) using primer pair 5’-CACCATGGATTTATGGCCTGGTGC-3’ & 5’-TCATCCTCCTCCACCTGG-3’ ...
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bioRxiv - Neuroscience 2023Quote: ... DNA oligonucleotides (RT-qPCR primers) were obtained from Merck (Rehovot, Israel).
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bioRxiv - Genomics 2023Quote: Saccharomyces cerevisiae S288C genomic DNA (69240-3) was purchased from Merck, Gillingham ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was precipitated by adding 300µl of 100% Ethanol (Merck) and centrifuged at 15,000g for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Plant Biology 2019Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2019Quote: ... using protein at 18 mg/ml (measured by Direct Detect® spectrometer (Merck)) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Immunology 2021Quote: 5 µl of 10 µm bead slurry (PureProteom Protein A magnetic beads, Merck) were used per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with Protein A Sepharose beads (Merck, GE17-5280-01) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected by Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, MA, USA) and a LAS-3000 mini chemiluminescence imaging system (Fujifilm ...
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bioRxiv - Plant Biology 2021Quote: ... the hybridization with proper antibodies by SNAP i.d 2.0 Protein Detection System (Merck) was performed ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto low fluorescent Immobilon-FL membrane (0.45μm por size, Merck) using the Biorad wet-blot system (1.15h ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Microbiology 2021Quote: ... and the bacterial pellets were resuspended in BugBuster protein extraction reagent (Merck, Germany). The cell suspensions were incubated on a rotary shaker for 20 minutes ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved proteins were transferred onto a 0.45 m PVDF membrane (Immobilon, Merck) in ice-cold transfer buffer (25 mM Tris ...