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Citations for Merck :
4801 - 4850 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Synthetic Biology 2021Quote: ... the diet for both the transgenic and wild-type strains was supplemented with CuSO4•5H2O (1 mM, Merck, CAS-no: 7758-99-9). Flies were reared in a controlled environment room at 25.0 °C ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Biophysics 2022Quote: ... was washed three times in Tris-buffer (pH 7.48) and resuspended in 1 %w/v Pluronic® F-127 (Merck Chemicals GmbH, Germany) Tris-Buffer.
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... were taken and consisted each of 1 L pre-filtered (10μm isopore membrane filter to remove larger zooplankton – Millipore Merck, Darmstadt, Germany, TCTP 04700) seawater concentrated on a 0.22 μm pore size membrane filter (Millipore Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Biochemistry 2022Quote: ... was added and incubated for 1 min at room temperature with gentle shaking (100 mM stock was prepared from fresh H2O2 Merck 30% w/w).
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Biochemistry 2024Quote: ... CHAPS was added to the dialyzed sample to concentration of 1% after which the MSP was concentrated to 20-40 mg/ml in centrifugal filter (Merck, MWCO 30 kDa), flash frozen and stored at -80°C.
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Plant Biology 2024Quote: ... or standards (gallic acid in the range of 0–80 µg ml-1) were mixed with 200 µl of 10% Folin-Ciocalteu’s phenol reagent (Sigma-Aldrich, Merck, Burlington MA, USA) and incubated at 25°C in the dark for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Developmental Biology 2021Quote: ... Purity of our mitotic sample was confirmed in a small sample cytospined (Simport, #720-1962) and immunostained with anti-pHH3 (Merck, #06-570, 1:500) and DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Pathology 2019Quote: ... the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA, Darmstadt, Germany 51) or polyclonal anti-CG antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: Colorimetric pH measurement was performed for 12 independent pancreatic juice samples by applying 1 µL sample onto MColorpHast™ pH strips (Merck Millipore, MA, USA). To adjust the pH of pancreatic juice ...
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Plant Biology 2022Quote: ... Spore concentration was counted via haemocytometer and adjusted to 1 x 105 spores per mL in 0.1% gelatin or dH2O with 0.01% Tween 20 (Merck Chemicals, CAS Number: 9005-64-5).
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Microbiology 2024Quote: To levels of cytokines in the sera of infected animals were obtained using the MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay Kit (Merck-Millipore, BCYT1-33K) and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: OncoTICE® vials were resuspended in sterile sodium chloride 0.9% solution at the Day Hospital (Champalimaud Foundation) according to the manufacturer instructions (Merck: 1 vial/50mL saline solution). Remnants from resuspended vials were stored at 4°C and protected from light.
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bioRxiv - Developmental Biology 2024Quote: ... and cultured as described in 39 using the following procedure: Cord blood samples were diluted 1:2 in PBS and layered on Ficoll® Paque Plus (Sigma-Aldrich by Merck, Darmstadt, Germany) to isolate mononuclear cells ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked with 5% milk in Tris buffered saline (TBS) and probed with antibodies to HCMV IE1 and IE2 (Merck Millipore; MAB-8131; 1/5000), pp52 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2019Quote: The level of RET phosphorylation in the cells was analyzed by Western blotting of immunoprecipitated RET with phospho-tyrosine-specific antibody (1:1500 Merck Millipore Cat# 05-321, RRID:AB_309678) as described previously (Leppanen et al. ...
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bioRxiv - Cell Biology 2019Quote: ... All mutations were confirmed by Sanger DNA sequencing and expression was confirmed by western blotting with anti-Myc primary antibody (1:2000 dilution; Merck Millipore #06-340, Billerica, MA).
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bioRxiv - Developmental Biology 2021Quote: ... The calcium-free ringer solution was replaced with 1 mL of protease solution (72 μg/mL Liberase DH research grade from Merck/Sigma, 0.4U/mL DNaseI-Invitrogen). The embryos were incubated at 28.5C on an orbital shaker for 20 minutes ...
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bioRxiv - Paleontology 2023Quote: An area of 1 cm² from bone artifacts was rubbed with a small swab (Foamtec UltraSOLV™ 1700 Series Swabs, Merck Life Science S.A.S, France) on a flat section of the artifact’s surface ...
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bioRxiv - Molecular Biology 2024Quote: ... Migrated PBMCs were further processed according to the manufacturers protocol and stained with CyQuant GR Dye diluted at 1/75 (Merck, Sigma-Aldrich, Cat. No. ECM512). Fluorescence was measured at 480 ± 15 nm (excitation ...
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bioRxiv - Molecular Biology 2024Quote: ... the eggs were transferred into centrifuge tubes containing 1 ml XBE2 + 10 µg/ ml protease inhibitor (aprotinin Sigma A6279, leupeptin Merck 108975, pepstatin Carl Roth 2936.1) + 100 µg/ ml cytochalasin D (Sigma C8273) ...
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bioRxiv - Plant Biology 2021Quote: ... the washed seedlings were transferred into TetSee X solution (15% Na-deoxycholate, 25% urea, 10 % glycerol, 5% 2’2-thiodiethanol [Merck, Product No. 166782], 1% Triton X-100) and kept for 3 days at 4°C with daily changes of the TetSee X solution ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...