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Citations for Merck :
4701 - 4750 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... pH 7.0) containing the cOmpleteTM EDTA-Free protease inhibitor at a 1 x concentration following manufactureŕs instructions (Merck Millipore, Darmstadt, Germany). The buffer volume was proportional to the cell density of the sample ...
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bioRxiv - Microbiology 2023Quote: ... the tetracycline resistance gene from pACE2 (Geneva Biotech, Genève, CH) and the two MCSs from pACYCDuet™-1 (Novagen, Merck Millipore). Csx23 was inserted into MCS-1 of pRATDuet (NcoI/SalI ...
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bioRxiv - Immunology 2023Quote: Bovine cytokines and chemokines secreted in the supernatant by cells were measured using the commercial multiplex immunoassay MILLIPLEX MAP Bovine Cytokine/Chemokine Panel 1 (Merck/Sigma). Therefore ...
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bioRxiv - Cell Biology 2023Quote: ... Transfectants with stably integrated GOI were then selected based on their resistance to G418 (0.5 mg/ml, 1-2 weeks, Merck, Cat.N. G8168). To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The chromatin pellet was then digested in 100 μl of water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 min at 37°C in a thermomixer at 1,400 rpm ...
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bioRxiv - Microbiology 2023Quote: ... the solution was incubated at 30 °C for 90 min and then derivatized with 20 µl of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchlorosilane (Merck, USA) at 70 °C for 30 min54 ...
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bioRxiv - Cell Biology 2023Quote: Sections were rinsed in 0.1 M PBS three times each for 10 min and then incubated with: i)1:1000 anti-NeuN (A-60; Merck Millipore), 1:300 anti-ΠIII-Tubulin (Tuj1 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The purity of the cultures was be routinely assessed by examining the characteristic cell morphologies under phase-contrast microscopy and confirmed by immunostaining with mouse anti-GFAP (1:200; Merck, MAB3402) and mouse anti-S100β (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: Astrocytes for long-term neuronal cultures were prepared by dissociating newborn rat cortices with 2.5% trypsin and 1 mg/ml DNase (Merck, cat# 10104159001) and plating the cells in MEM with L-glutamine supplemented with 0.6% glucose ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated in a 10% (v/v) HS PBS solution supplemented with primary mouse anti-β-tubulin III antibody (1:100; Merck) overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... The routinely used growth medium was composed of yeast extract (1%(w/v)) (Fisher BioReagents™) and casein peptone (2%(w/v)) (Merck), and was supplemented with 2% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated for 1 hour at room temperature with the anti-NOTCH1 primary antibody (Merck Life Sciences, Cat #: SAB4200024) diluted at 1:350 in 1% BSA blocking solution ...
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bioRxiv - Systems Biology 2023Quote: ... Media was designed based on the Delft composition with a glucose concentration of 10 g/L and the addition of 1 mL/L of Antifoam 204 (Merck, Germany).
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bioRxiv - Microbiology 2023Quote: Washed DPI-treated VBNC Mtb cells were resuspended with fresh Dubos broth and the suspension was diluted to 1:10 with Dubos broth with 0.1% (w/v) BSA containing adenylyl cyclase inhibitor SQ22536 (Merck, Darmstadt, Germany) or protein kinase inhibitor H89 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... 20 µL of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2x final concentration of Protease Inhibitor Cocktail Set 1 (Merck Chemicals). After centrifugation (5 min 15000g) ...
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bioRxiv - Neuroscience 2023Quote: Place 100 μl trypsin and 100 μl collagenase (per 2 animals) in a 35 mm dish: 2 mg ml-1 Trypsin (Merck - 85450C) and 682 U ml−1 Collagenase in Ca2+ and Mg2+ free medium
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bioRxiv - Plant Biology 2023Quote: A barrel of a 1 mL needleless syringe is filled with 800 µl of an aqueous (double-distilled water) ‘infiltration solution’ that contains 1 mM of the fluorophore pyranine (pKa in aqueous solution: 7.3; Merck, Darmstadt, Germany). The water serves for extracting the apoplastic solutes ...
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bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed them six times for 10 min with 1 % Triton (Triton X-100, X100-100ML, Sigma Aldrich, Merck, Germany) in PBS (thereafter named 1 % PBST ...
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bioRxiv - Cancer Biology 2023Quote: The Propidium Iodide (PI) working solution was prepared consisting of 4.35 mL 1 g/L sodium citrate dehydrate (Merck, cat# 6448), 0.5 mL 1 mg/mL DNAse-free RNAase A (Sigma ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Both the supernatant and the resuspended pellet were incubated with 1 U/µL of benzonase (Emprove expert benzonase endonuclease, Merck #101695) at 37 °C for 1 h with gentle shaking to digest non-encapsidated DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1:100, in-house antibody SCV-1E8 (Morgan et al., 2023)) in conjunction with either anti-NeuN (chicken, 1:3000, Merck, ABN91), anti-Iba1 (rabbit ...
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bioRxiv - Plant Biology 2023Quote: Plants were grown on 1/2 MS agar plates containing 0.01% (v/v) dimethyl sulfoxide (mock) or 50 ng mL-1 TM (654380; Merck, Darmstadt, Germany) for 14 days ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... ACN and then rehydrated with 30 µL of 3.33 µg × mL-1 trypsin (cat-no. T6567 from Sigma-Aldrich/Merck, www.sigmaaldrich.com) in 25 mM ABC ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by approx ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin pellet was then digested in 100 μl of water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 min at 37°C in a thermomixer at 1,400 rpm.
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bioRxiv - Cancer Biology 2024Quote: ... The shRNA mediated knockdown of the respective EWSR1::ETS fusion oncogenes was induced by addition of 1 μg/mL dox (Merck, Germany) to the cell culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Neuroscience 2024Quote: ... fish were briefly rinsed in water and embedded in 1% agarose (Carl Roth, Germany) in 1.8 ml vials (Nunc CryoTube Vials, Merck (Sigma-Aldrich), Germany) ...
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bioRxiv - Microbiology 2024Quote: ... ParT (S48C)-His6 and ParT (Q271C)-His6 aliquots were supplemented with 1 mM tris (2-carboxyethyl) phosphine hydrochloride (Merck; cat# C4706) before flash-freezing in liquid nitrogen.
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bioRxiv - Plant Biology 2019Quote: ... The samples were fractionated using an Alliance 2795 HPLC system (Waters) and µLC column (SeQuant ZIC®-HILIC, 15 cm x 1 mm internal diameter, 5 µm, 200 Å, Merck), with a mobile phase consisting of solvent A (10 % 10 mM ammonium acetate pH 7.3 90 % acetonitrile ...
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bioRxiv - Biophysics 2020Quote: mamM CTD gene from Magnetospirillum gryphiswaldense MSR-1 (UniProt Q6NE57 residues 215-318) was previously cloned into pET28a(+) vector (Novagen, Merck Biosciences, Germany)22 ...
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bioRxiv - Immunology 2021Quote: ... wells washed with 1 X PBS and incubated with 50 µL of incomplete media containing 5 µM Laurdan (Avanti® Polar Lipids, Merck, USA) for 30 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... C57BL/6 ESCs were transduced with the lentivirus at a multiplicity of infection (MOI) ranging from 0.1 to 1 with 5 μg/μL polybrene (Merck Millipore, Cat# TR1003G). From day three onward (e.g ...
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bioRxiv - Biochemistry 2019Quote: A CRISPR array containing three Mtb repeat sequences flanking two identical spacer sequences targeting the pUC19 multiple cloning site was assembled by BamHI digest of the double-stranded mtbCRISPR array oligonucleotide (Table S2) followed by ligation into pCDFDuet™-1 (Novagen, Merck Millipore). Mtb cas6 was cloned into MCS-2 to give pCRISPR.
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Genomics 2021Quote: ... The cloned shRNAs against the respective EWRS1-ETS fusion oncogenes was induced by addition of 1 µg/ml dox (Merck, Darmstadt, Germany) to the cell culture medium ...