Labshake search
Citations for Merck :
4751 - 4800 of 4853 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... and the remaining skin was cut in strips of approximately 4 cm length and 1 mm width and finally incubated in 10 ml skin digestion solution (2.0 U/mL dispase D4693, Merck KGaA, in PBS-) at 4°C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... After adding 1 drop of bovine thrombin (Biomed, Lublin, Poland) to 2 drops of fibrinogen (1mg/ml; Sigma-Aldrich, St. Louis, MO; Merck KGaA) the cells were entrapped within the fibrin clots ...
-
bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
-
bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
-
bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... Immunostaining was performed on retinal cryosections by incubating with rabbit primary antibodies against cone arrestin (1:1000; catalog AB15282, Merck Chemicals GmbH), mouse anti-rhodopsin (1:400 ...
-
bioRxiv - Cell Biology 2021Quote: ... ethanol and cut into 1–2 mm3 pieces and was cultured in Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, Merck, Darmstadt, Germany) with 10% (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
-
bioRxiv - Biochemistry 2022Quote: ... A BglII-XbaI fragment containing His8-MBP-NOT10 was then cut out of the pnEK vector and cloned between the BglII and AvrII restriction sites behind the second T7 promoter of the pETDuet-1 vector (Merck, Darmstadt, Germany). The resulting plasmid was cut with SalI and BglII ...
-
bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
-
bioRxiv - Systems Biology 2022Quote: ... the proteins were alkylated for 30 min or 1 hour at room temperature in the dark by addition of 15 mM iodoacetamide (Merck Sigma, I1149) or 50 mM acrylamide (AA ...
-
bioRxiv - Neuroscience 2022Quote: ... Compounds 1-10 were purified by flash chromatography on high-purity grade silica gel (9385, 60 Å, 230-400 mesh) from Merck (Germany), producing hydrochlorides in the form of white powders.
-
bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
-
bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
-
bioRxiv - Cell Biology 2022Quote: ... and incubated with amino acid-free and FBS-free DMEM (Wako Pure Chemical Industries, 048-33575) to induce autophagy by starvation or to DMEM with 1 µM oligomycin (Merck Millipore, 495455) and 2 µM antimycin (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2019Quote: General transformation procedure as described procedure A but using RbCl chemo-competent BL21(DE3) pLysS (aliquot of 100 µl; 2.4 × 107 cfu µg−1 pUC19 DNA, Merck Millipore, Darmstadt, DE). Transformed cells were plated (50 µl final volume ...
-
bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
-
bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The membranes were probed with anti-GFP at a dilution of 1:3000 of the antibody conjugated to a peroxidase (SAB2702198, Merck KGaA, Germany) in the blocking buffer overnight at 4 °C on a rocker ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
-
bioRxiv - Immunology 2021Quote: ... Cartilage was dissected from the tibial plateau and minced before overnight collagenase digestion in DMEM-F12 supplemented with 1% P/S and 1mg/ml collagenase IA (Sigma, supplied by Merck, Darmstadt, Germany). Synovium was minced before being collagenase digested for 2 hours ...
-
bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
-
bioRxiv - Plant Biology 2022Quote: ... disposable 1000 μl pipette tips were fitted with 4 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1 mg:10 μg of LiChroprep® RP-18 (Merck) ...
-
bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting supernatant containing the MCR-1 solubilized in DDM detergent micelles was filtered using an 0.22 µm syringe filter (Merck Millipore, Darmstadt, Germany) and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... The collected fractions were analysed on SDS-PAGE and fractions containing the protein complex of interest at the expected stoichiometry were pooled and concentrated to a concentration of 1-2 mg/ml using an Amicon ultra 30K centrifugal filter (Merck, cat UFC903024). The purified protein was then aliquoted and frozen in liquid nitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... DLD-1 cells expressing different EPHB1 wildtype or mutant versions and EFNB1 were mixed in suspension at a ratio of 1:3 and plated at a density of 130,000 cells/cm2 on coverslips coated with 2 mg/cm2 of 1-2 mg/mL laminin (Merck KGaA, Germany, USA) and incubated at 37ºC in 5% CO2 ...
-
bioRxiv - Plant Biology 2023Quote: ... The combined aqueous phase gave a total volume of 450 mL of which 5 uL were measured by Method 1 and compared against freshly prepared authentic standards (Merck, Darmstadt, Germany). We wish to note that our acid quantification generated much higher absolute values than an earlier study (Szecowka et al ...
-
bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
-
bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
-
bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
-
bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP signals were amplified by immunostaining with polyclonal rabbit anti-GFP (used at 1:500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, SAB4301138). 40-1a (used at 1:50 ...
-
bioRxiv - Biophysics 2023Quote: ... Amphipol-reconstituted P2X4 was pooled and concentrated to approximately 1 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All steps were performed at 4 °C.
-
bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
-
bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Base elastomer was thoroughly mixed with curing agent at a ratio of a ratio of 10:1, before degassing in a vacuum desiccator (Scienceware, 999320237) with a KNF Laboport N96 diaphragm vacuum pump (Merck, Z675091-1EA). A Si wafer with positive features was placed in a disposable aluminium dish (VWR ...
-
bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
-
bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
-
bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
-
bioRxiv - Plant Biology 2024Quote: Visualization of intracellular and apoplastic ROS in guard cells was performed using 20 µM H2DCFDA or 100 µM peroxy orange 1 for H2O2 (PO1; Merck Sigma-Aldrich), and 100 µg/ml Oxyburst Green H2HFF-BSA (Thermo Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
-
bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
-
bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...