Labshake search
Citations for Merck :
4451 - 4500 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
-
bioRxiv - Biochemistry 2022Quote: ... A BglII-XbaI fragment containing His8-MBP-NOT10 was then cut out of the pnEK vector and cloned between the BglII and AvrII restriction sites behind the second T7 promoter of the pETDuet-1 vector (Merck, Darmstadt, Germany). The resulting plasmid was cut with SalI and BglII ...
-
bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
-
bioRxiv - Systems Biology 2022Quote: ... the proteins were alkylated for 30 min or 1 hour at room temperature in the dark by addition of 15 mM iodoacetamide (Merck Sigma, I1149) or 50 mM acrylamide (AA ...
-
bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
-
bioRxiv - Neuroscience 2022Quote: ... Compounds 1-10 were purified by flash chromatography on high-purity grade silica gel (9385, 60 Å, 230-400 mesh) from Merck (Germany), producing hydrochlorides in the form of white powders.
-
bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
-
bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
-
bioRxiv - Cell Biology 2022Quote: ... and incubated with amino acid-free and FBS-free DMEM (Wako Pure Chemical Industries, 048-33575) to induce autophagy by starvation or to DMEM with 1 µM oligomycin (Merck Millipore, 495455) and 2 µM antimycin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
-
bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The membranes were probed with anti-GFP at a dilution of 1:3000 of the antibody conjugated to a peroxidase (SAB2702198, Merck KGaA, Germany) in the blocking buffer overnight at 4 °C on a rocker ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
-
bioRxiv - Immunology 2021Quote: ... Cartilage was dissected from the tibial plateau and minced before overnight collagenase digestion in DMEM-F12 supplemented with 1% P/S and 1mg/ml collagenase IA (Sigma, supplied by Merck, Darmstadt, Germany). Synovium was minced before being collagenase digested for 2 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
-
bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
-
bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
-
bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
-
bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting supernatant containing the MCR-1 solubilized in DDM detergent micelles was filtered using an 0.22 µm syringe filter (Merck Millipore, Darmstadt, Germany) and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
-
bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
-
bioRxiv - Plant Biology 2022Quote: ... disposable 1000 μl pipette tips were fitted with 4 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1 mg:10 μg of LiChroprep® RP-18 (Merck) ...
-
bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP signals were amplified by immunostaining with polyclonal rabbit anti-GFP (used at 1:500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, SAB4301138). 40-1a (used at 1:50 ...
-
bioRxiv - Biophysics 2023Quote: ... Amphipol-reconstituted P2X4 was pooled and concentrated to approximately 1 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All steps were performed at 4 °C.
-
bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Base elastomer was thoroughly mixed with curing agent at a ratio of a ratio of 10:1, before degassing in a vacuum desiccator (Scienceware, 999320237) with a KNF Laboport N96 diaphragm vacuum pump (Merck, Z675091-1EA). A Si wafer with positive features was placed in a disposable aluminium dish (VWR ...
-
bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
-
bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
-
bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
-
bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
-
bioRxiv - Plant Biology 2024Quote: Visualization of intracellular and apoplastic ROS in guard cells was performed using 20 µM H2DCFDA or 100 µM peroxy orange 1 for H2O2 (PO1; Merck Sigma-Aldrich), and 100 µg/ml Oxyburst Green H2HFF-BSA (Thermo Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: For GC-MS analysis all the samples were derivatized with N,O- Bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA) purchased from Merck (Darmstadt, Germany).
-
bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
-
bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
-
bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
-
bioRxiv - Bioengineering 2024Quote: ... The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm2/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin was then sheared either by passaging 10 times through a 27G needle attached to a 1 ml syringe or by the addition of Benzonase® Nuclease (Merck, UK). Needle-sheared nuclear fractions were centrifuged at 13,000 x g for 10 minutes (4°C ...
-
bioRxiv - Biophysics 2024Quote: ... The membrane pellet was resuspended in extraction buffer (25mM MOPS-NaOH, 150mM NaCl, 50mM EDTA, 10mM DTT, 0.1 mg/ml lysozyme, 1% lauroylsarcosine (Merck, Cat. No. L5777), pH 7.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...