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Citations for Merck :
4701 - 4750 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MCAD activity was measured using 3-phenylpropionyl-CoA (custom synthesis; 0.3 mM) as substrate and ferrocenium hexafluorophosphate as electron acceptor (Merck, 388297; 1.5 mM), followed by detection of 3-phenylpropenonyl-CoA using UHPLC (Wanders ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenized tissue was centrifuged at 16,000 g for 45 min at 4 °C and the remaining supernatant was spun in 3 kDa cut-off Amicon® centrifugal filter tubes (Merck Millipore) at 14,000 g for 90 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of culture was sonicated for 3 s and 200 μL were loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Merck Millipore) connected to the CellASIC® microfluidic pump (Merck Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were removed from the samples by ultrafiltration at 3000 g and 30 min using Amicon Ultra filters (3 kDa cut-off, Merck, Darmstadt, Germany). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... Purity of our mitotic sample was confirmed in a small sample cytospined (Simport, #720-1962) and immunostained with anti-pHH3 (Merck, #06-570, 1:500) and DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Microbiology 2021Quote: Colorimetric pH measurement was performed for 12 independent pancreatic juice samples by applying 1 µL sample onto MColorpHast™ pH strips (Merck Millipore, MA, USA). To adjust the pH of pancreatic juice ...
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Microbiology 2024Quote: To levels of cytokines in the sera of infected animals were obtained using the MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay Kit (Merck-Millipore, BCYT1-33K) and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Cancer Biology 2024Quote: OncoTICE® vials were resuspended in sterile sodium chloride 0.9% solution at the Day Hospital (Champalimaud Foundation) according to the manufacturer instructions (Merck: 1 vial/50mL saline solution). Remnants from resuspended vials were stored at 4°C and protected from light.
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bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase, Merck, dissolved in TIRF buffer), 45% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled fractions were concentrated to 20 mg mL-1 using a 10-kDa cut off filtration membrane (Cat. UFC501096, Merck Millipore, Burlington, MA, USA). Sitting drop crystallization trials were set up by depositing 150 nL of the precipitant solution on top of 150 nL of protein with 70 μL of the reservoir using a Crystal Gryphon LCP robot (Art Robbins Instruments) ...
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bioRxiv - Developmental Biology 2021Quote: ... Metabolite samples (2.5 μL) were separated using a ZIC-pHILIC stationary phase (2.1 × 150 mm, 5 μm) (Merck). The autosampler temperature was 4 °C and the column compartment was maintained at 25 °C ...
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bioRxiv - Cancer Biology 2022Quote: Mayer’s haematoxylin solution was prepared by dissolving 5 g of aluminium potassium sulphate dodecahydrate (Merck Millipore, cat. 1010421000) in 100 mL of water ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then filtered through 5-kDa cut-off filters (Ultrafree-MC PLHCC, Merck KGaA, Darmstadt, Germany) to remove macromolecules ...
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bioRxiv - Bioengineering 2021Quote: ... Cetuximab antibody (Erbitux infusion solution, 5 mg/mL) was used to target EGFR and was purchased from Merck Serono ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 ml HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2021Quote: Putative promoter sequences were amplified from genomic DNA using the KOD Hot start polymerase (#71086-5, Merck Millipore) and cloned into pJET1.2 (#K1231 ...
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bioRxiv - Microbiology 2021Quote: ... A SeQuant ZIC-pHILIC (2.1 × 100 mm, 5 μm particles) HILIC phase analytical column (Merck KGaA, Darmstadt, Germany) was used as a chromatographic separation column.
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bioRxiv - Microbiology 2020Quote: ... prausnitzii grown for 24 h were centrifugated for 5 min at 5,000 x g and the supernatant was filtered through 0.22 μm filters (Merck). Then 100 μl of the cleared bacterial supernatants (or fresh Y-BHI medium alone ...
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bioRxiv - Microbiology 2022Quote: ... and SAM_Seq_Gen59708_RV (5’-ACA AGA GGC GGC TTT ATG TTC C) together with KOD Hot Start DNA Polymerase (Merck). Additionally ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants of BMDMs were 5-fold concentrated using the Amicon Ultra-0,5 Membrane Ultracel-10 columns (Merck Millipore) and the IFNβ concentration was evaluated using the VeriKine Mouse Interferon Beta HS ELISA Kit (PBL assay science).
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bioRxiv - Cell Biology 2021Quote: ... G2500N) coated with Formvar film and stained 20 min with 5% uranyl acetate (Merck, Darmstadt, Germany, cat. 8473) and 5 min Reynolds lead citrate ...
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bioRxiv - Plant Biology 2021Quote: ... The probes were incubated in reaction buffer (5 mM CoCl2, 400 U/reaction of Terminal Transferase (Sigma Merck) 0.1 mM DIG-11-dUTP (Sigma Merck) ...
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bioRxiv - Molecular Biology 2020Quote: ... and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v) (Merck, Roth) in a developing chamber (CAMAG ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
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bioRxiv - Genomics 2021Quote: ... with A pestle ~10 times following B pestle ~20 times in lysis buffer [5 mM CaCl2 (Merck, A546282), 3 mM Mg(Ac)2 (Roth ...
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bioRxiv - Microbiology 2020Quote: ... followed by a mid-filter of 5 μm pore size (Mixed cellulose ester membrane filter; Merck Millipore, USA) and finally through a 0.22 μm collection filter (Cellulose mixed ester membrane filter ...
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bioRxiv - Cell Biology 2021Quote: ... or 2500 cells/well depending on PARPi dose in 5 ml of medium supplemented with 1.5% methylcellulose (Merck) and the indicated concentrations of Olaparib ...
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bioRxiv - Immunology 2021Quote: ... Frozen tissues were sectioned to a thickness of 5 μm and fixed with 4% paraformaldehyde (Merck, Kenilworth, NJ). Slides were incubated with 1% BSA in PBST for 1 h to block the non-specific antibody binding ...
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bioRxiv - Biophysics 2022Quote: ... The PVDF membrane was blocked in 5 w/v% milk (skim milk powder for microbiology, Millpore, Merck KGaA) in PBS for 1 hour on an orbital shaker (SC5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The ChIP experiments were performed using the following antibodies: CTCF (07-729, Merck Millipore, 5 ml per ChIP), RAD21 (ab154769 ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolite samples (2.5 μL) were separated using a ZIC-pHILIC stationary phase (2.1 × 150 mm, 5 μm) (Merck). The autosampler temperature was 4 °C and the column compartment was maintained at 25 °C ...
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bioRxiv - Microbiology 2022Quote: ... were concentrated to 5 mL by 10 kDa Amicon® Ultra Centrifugal Filters (Lot No. R9EA18187, Merck Millipore) and then applied for CsCl density gradient centrifugation at 24,000 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... T865.3) was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µg/mL leupeptin) supplemented with 250 µg/mL lysozyme (from hen egg white; Merck KGaA, Darmstadt, Germany) and incubated on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... For liquid chromatography a SeQuant ZIC-pHILIC 5 μm Polymeric column 150 * 2.1 mm (Merck Millipore, Darmstadt, Germany) was used ...