Labshake search
Citations for Merck :
4301 - 4350 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 20S proteasome activity as well as reactive oxygen species production, 300 µl Tris-Buffered Saline (TBS, pH 7.6) and 1% Triton X-100 (Merck, Darmstadt, Germany) were added to a tissue powder aliquot and homogenization was performed using ten consecutive passages through a 24-gauge needle on ice ...
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bioRxiv - Physiology 2022Quote: ... To generate protein homogenates suitable for western blot analysis, 300 µl Tris-Buffered Saline (TBS, pH 7.6) + 1% Triton X-100 (Merck, Darmstadt, Germany) supplemented with 1x Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... yeast of cell line BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) was grown in 1 litre of YPD (yeast extract (Merck/Sigma-Aldrich cat. no. 70161), peptone (Merck/Sigma-Aldrich cat ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at 4°C with primary antibodies against AVP neurophysin II (NP-II; MERCK, MABN845, PS41; 1:200); OXT NP-I (PS38 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with a BG4-Flag antibody (1:100 diluted in 0.5% goat serum + PBST, MABE917, Merck, Darmstadt, Germany) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Microbiology 2023Quote: Washed DPI-treated VBNC Mtb cells were resuspended with fresh Dubos broth and the suspension was diluted to 1:10 with Dubos broth with 0.1% (w/v) BSA containing adenylyl cyclase inhibitor SQ22536 (Merck, Darmstadt, Germany) or protein kinase inhibitor H89 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: A barrel of a 1 mL needleless syringe is filled with 800 µl of an aqueous (double-distilled water) ‘infiltration solution’ that contains 1 mM of the fluorophore pyranine (pKa in aqueous solution: 7.3; Merck, Darmstadt, Germany). The water serves for extracting the apoplastic solutes ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by approx ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Plant Biology 2024Quote: ... Blotted proteins were then visualised using goat anti-rabbit HRP secondary antibody (Thermo Fischer Scientific) at dilution 1: 900 and ECL Western HRP substrate (Merck Millipore).
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bioRxiv - Pathology 2024Quote: Quarter kidneys stored at −80°C were placed in 200 µl RIPA buffer (150 mM NaCl, 10 mM Tris HCl, 1% Na deoxycholate, 0.1% SDS) with protease inhibitor (Merck, cat# 05892791001). To this ...
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bioRxiv - Systems Biology 2024Quote: ... Media was designed based on the Delft composition with a glucose concentration of 9 g/L and the addition of 1 mL/L of Antifoam 204 (Merck, Germany). The bioreactors were inoculated at an OD600 of 0.1 ...
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bioRxiv - Immunology 2024Quote: ... deparaffination was followed by 1-hour incubation in room temperature with the primary antibody (monoclonal rat, MAB386; Merck Millipore, Darmstadt, Germany) diluted 1:800 in dilution buffer (Agilent Dako ...
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bioRxiv - Microbiology 2024Quote: ... a red tetrazolium salt that turns yellow when cleaved to formazan by mitochondrial dehydrogenases (WST-1 Assay Protocol for Cell Viability, MERCK®). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin pellet was then digested in 100 μl of water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 min at 37°C in a thermomixer at 1,400 rpm.
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bioRxiv - Cancer Biology 2024Quote: ... The shRNA mediated knockdown of the respective EWSR1::ETS fusion oncogenes was induced by addition of 1 μg/mL dox (Merck, Germany) to the cell culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Neuroscience 2024Quote: ... fish were briefly rinsed in water and embedded in 1% agarose (Carl Roth, Germany) in 1.8 ml vials (Nunc CryoTube Vials, Merck (Sigma-Aldrich), Germany) ...
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid A samples were resuspended in 30 μl of chloroform/methanol mixture (4:1) for thin-layer chromatography (TLC) analysis using Silica Gel 60 F254 TLC plate (Merck Millipore) with the solvent system consisting of chloroform/pyridine/96% formic acid/water (50/50/14.6/4.6) ...
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bioRxiv - Microbiology 2024Quote: SFPQ and FLAG RIPs were performed in TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck Millipore) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were incubated for 90 minutes at room temperature: anti-rabbit Alexa Fluor® 647 (1:800; Merck Millipore, #AP187SA6), anti-mouse Alexa Fluor® 488 (1:800 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 300 µg of protein was then incubated with 1 µg of a mouse anti-53BP1 antibody (1μg/ml, Merck millipore, MAB3802) O/N at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... LCC and Fast PETase. Single colonies (E. coli XJb (DE3)) were cultured in 1 mL of overnight autoinduction media (Merck, USA) supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biophysics 2024Quote: ... and resuspended in 1 mL PBS supplemented with protease inhibitor and protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Biochemistry 2024Quote: ... These sections were then treated with 1 mL of artificial cerebrospinal fluid which composed of 120mM NaCl (Merck Cat. No. S9888), 2.5 mM KCl (Merck Cat ...
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bioRxiv - Cell Biology 2024Quote: ... SRM medium was supplemented with same concentrations of LDN and SB and adding 1 μM of Smoothened Agonist (SAG; Merck Millipore), 2 μM of Purmorphamine (Tocris Bioscience) ...
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bioRxiv - Developmental Biology 2024Quote: ... the lung distal regions were cut into small pieces and dissociated in 5 mL of enzyme mixture (0.125 mg/mL Collagenase, Merck, C9891; 1 U/mL Dispase, Thermo Fisher Scientific, 17105041; 10 U/mL DNAase, Merck, D4527) at 37°C for 1 hour with rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... All ingredients of the “Amino acid mixture” (Supplemental Table 1) were weighed out and added to a glass beaker containing ultrapure water (Milli-Q Water Purification System, Merck Millipore). Tyrosine was separately pre-dissolved in 2.8 M KOH ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Biophysics 2020Quote: ... 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 µm pores (Cat # 353096, Merck). The inserts were coated with rat-tail collagen I ...
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bioRxiv - Cell Biology 2020Quote: ... Viruses were collected from the supernatant 24 hours later and cleared by centrifugation at 2000 rpm for 3 minutes followed by filtration with 0.45 µm PVDF syringe filter units (Merck, #SLHV033RS). Cleared supernatants were titrated by plaque assay using BHK-21 cells.
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bioRxiv - Evolutionary Biology 2020Quote: Larvae were raised as described above until 11 dpf and deeply anesthetized with 160 mg/l of Tricaine (Ethyl 3-aminobenzoate methanesulfonate salt, MS-222, Merck) before dissecting their pectoral fins ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were permeabilized with 0.1 % Triton-X100 in PBS for 20 min at RT and samples were blocked with freshly prepared 3 % bovine serum albumin (BSA, Merck) in PBS for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: Heat-mediated antigen retrieval was performed for 3 min at 99°C in a 40mM trisodium citrate (Merck, Darmstadt, Germany) solution ...
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bioRxiv - Biochemistry 2020Quote: ... Chromatographic separation of AAs was achieved by applying 3 μl of dissolved sample on a SeQuant ZIC-HILIC column (3.5 μm particles, 100 × 2.1 mm) (Merck, Darmstadt, Germany), combined with a Javelin particle filter (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Cells were sonicated at low power for 3 s and loaded onto a commercial microfluidics system (Y04C-02 plates, CellASIC ONIX2 system, Merck). While loading the cells ...
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bioRxiv - Biochemistry 2020Quote: ... each N-Cdh sample was desalted against PBS using Amicon centrifugal filters with a 3 kDa molecular weight cutoff according to the manufacturer’s protocol (Merck-Millipore). Unprocessed xCGE-LIG N-glycocomics raw data are available upon request.
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bioRxiv - Bioengineering 2021Quote: ... 99%+, Figure S1(d)), and dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP, 42% solution in methanol) were obtained from Merck–Sigma Aldrich ...