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Citations for Merck :
4101 - 4150 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 10 % (v/v) glycerol, 1% (w/v) digitonin (Carl Roth GmbH, Karlsruhe, Germany) and cOmplete protease inhibitor (Merck). Samples were rotated on a wheel for 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary incubation was completed with a 1:40 dilution of Goat anti-Rabbit IgG 10 nm gold conjugate for a further hour (Merck).
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bioRxiv - Immunology 2024Quote: Splenocytes were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Plant Biology 2024Quote: ... AtPFA-DSP1 and AtNUDT17 were resuspended in buffer A (50 mM Tris pH 7.5, 500 mM NaCl, 1 mM DTT, Dnase I and cOmplete™ protease inhibitor cocktail [Merck]), MpPFA-DSP1 and MpNUDT1 were resuspended in buffer B (50 mM K2HPO4/KH2PO4 pH 7.8 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: DNA encoding the TBD domain of human ASK1 (residues 88–267) was ligated into pRSFDuet-1 (Merck KGaA, Darmstadt, Germany) using BamHI and NotI sites ...
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Genetics 2024Quote: ... Cells were washed with water thrice and released into YPD media containing 15 µg mL-1 nocodazole (Cat. No. M1404, Merck) for 90 min ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 2.5 µg mL-1 α-factor grown at 25°C (Cat. No. T6901, Merck). After 90 min ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were fixed in 1% glutaraldehyde in 1x PBS for 30 min and stained with filtered 10% Giemsa (Merck) in 1x PBS for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR was performed on 1 μl of each library with the KAPA SYBR FAST qPCR Master Mix (Merck, KK4600) to estimate the number of PCR cycles required to amplify each library ...
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bioRxiv - Molecular Biology 2024Quote: Coverslips were transferred to a humid chamber and incubated overnight with the primary antibody diluted in 1% BSA/PBS (anti-MAP2 antibody (AB554, Merck), 1/500 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 30 minutes and washed twice in PBS for 1 minute each prior to permeabilization: sections were briefly digested with 0.5 mg/ml pepsin (Merck P7012) in 0.1 M HCl (Fisher 10325710 ...
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bioRxiv - Microbiology 2024Quote: ... routine wash and control runs were done by injecting 5 µl LC-MS solution containing 5 µL of 10 fmol/µL BSA or enolase digest and 1 µL of 10 fmol/µL angiotensin III (Fluka, Thermo)/oxytocin (Merck) to check the performance of the platform on each component (nano-LC ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were plated in serum-free RPMI containing 10 mM sodium pyruvate for 1 hr prior to changing media to complete RPMI supplemented with 10% human serum (from human male AB plasma, Merck), 1 U/mL penicillin and 0.1 mg/mL streptomycin ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was pelleted by centrifugation at 3,000 g for 10 min at 4°C and the pellet was resuspended in 100 μl of 1% SDS lysis buffer (1% SDS in 50 mM Tris-HCl (pH=8.0) and supplemented with 1X protease inhibitor cocktail (from 100X; Merck, P8849), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated to ∼ 10 mg/mL (as judged by the extinction coefficient χ410= 213 mM-1cm-1) using Amicon 50K MWCO centrifugal concentrators (Merck). Dimeric NmqNOR was immediately used to make grids following SEC ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Microbiology 2024Quote: ... Peak fractions were concentrated to 1.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an incubation overnight at 4 °C in blocking solution with anti-puromycin antibody (clone 12D10, MABE343; 1:500) AlexaFluor 488 or AlexaFluor 647 (MABE343-AF488 or MABE343-AF647; Merck). On the following day ...
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bioRxiv - Neuroscience 2024Quote: ... Medium change was performed every 72 h by exchanging half of the volume of the medium with fresh NB medium with complements and AraC (1 μg/ml for the first, and 0.5 μg/ml for the second medium change, Sigma/Merck, Germany), cells were harvested at DIV7 (ChIPseq ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated at 4°C (48h) with the rabbit polyclonal primary antibody anti-TH (1:1000, Merck, Germany, #AB152), rinsed three times in 0.1 M PB ...
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bioRxiv - Biochemistry 2024Quote: Peak fractions from size-exclusion chromatography were concentrated to 4-6 mg mL-1 using a 50 kDa MWCO spin column (Merck), followed by addition of 30 mM D-glucose ...
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bioRxiv - Systems Biology 2024Quote: ... rehydrated sections [20] in a 800 ml glass container filled with the retrieval solutions (EDTA pH 8; 1 mM EDTA in 10 mM Tris-buffer pH 8, Merck Life Science S.r.l.,Milano ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies anti-acetylated tubulin (Merck, T7451, 1/2000); anti-Arl13b (Sanbio ...
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated overnight at 4°C with the primary antibodies: rabbit anti-NFH (1:1000, Merck millipore, Germany) and mouse anti-SMI312 (1:250 ...
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bioRxiv - Bioengineering 2024Quote: ... and seeded on four 60 mm dishes (3010-060, AGC Techno Glass) precoated with 1 µg/cm2 fibronectin (FC010, Merck) and cultured for 2 days in StemPro-34 SFM with 2 mM GlutaMAX ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase ...
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bioRxiv - Cell Biology 2024Quote: ... EV-enriched fractions (1-4) were pooled and concentrated using Amicon 15 Ultra RC 10 kDa MWCO filters (Merck Millipore). Expi293F-palmGRET EV aliquots in Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... routine wash and control runs were done by injecting 5 µl LC-MS solution containing 5 µL of 10 fmol/µL BSA or enolase digest and 1 µL of 10 fmol/µL angiotensin III (Fluka, Thermo)/oxytocin (Merck) to check the performance of the platform on each component (nano-LC ...
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bioRxiv - Cell Biology 2024Quote: ... organoid culture medium is removed and replaced by fresh medium containing the fluorescent probes to stain the DNA (Hoechst, 1:3000 v/v) and the tubulin (Biotracker 488 Microtubules, Merck, dilution 1:500 used with Verapamil 1:1000 to contain the probe inside the cells).
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then incubated for 1 hour in 10 ml of 80% THF/H2O and twice 1 hour in 100% THF and then in dichloromethane (DCM) (270997-12X100ML, Merck) until they sank at the bottom of the vial ...
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bioRxiv - Microbiology 2021Quote: ... MilliQ water was produced in-house using a Milli-Q Integral 3 (LC-Pak polisher, Millipak express 40 filters, 0.22 µm, Merck) for a final organic content < 3ppb ...
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Biochemistry 2021Quote: ... 2/3 of the resulting supernatant (RIPA-insoluble fraction) was filtered through a nitrocellulose membrane (0.2 μM pore size, Merck) using a filter trap slot blot (Hoefer Scientific Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
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bioRxiv - Physiology 2022Quote: ... Metabolites were chromatographically separated in a SeQuant ZIC-cHILIC column (PEEK 100 x 2.1 mm, 3 μm particle size; Merck) at 30°C using a method consisting of a gradient running at 0.25 mL/min from 100% mobile phase B (9:1 acetonitrile:water containing 5 mM ammonium acetate pH 6.8 ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was applied to a centrifugation filter (Amicon Ultra 0.5 mL 3 K device; Merck KGaA, Darmstadt, Germany), and the fraction containing free amino acids was obtained by centrifugation (15,000 × g ...
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bioRxiv - Biophysics 2021Quote: ... the dry lipid film was rehydrated with 3 mL of 0.2 M 8.5 pH Bicine (Merck Life Science, Norway) buffer (pH adjusted with NaOH (Merck Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... The LE11-27 DNA was dialyzed against the same buffer and concentrated to ~ 2.5 mM (Vivaspin 500, 3 kDa MWCO, Merck). The complex was formed by mixing the protein and the DNA in a 2:1 protein-to-DNA ratio (final concentration of 800 µM and 400 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Merck 200-664-3). None of the cell lines were authenticated but low passage numbers were utilized.
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...