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Citations for Merck :
4051 - 4100 of 4581 citations for 7 Bromo 2 4 fluoro phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with gene-specific siRNAs or a control siRNA (MISSION siRNA Universal Negative Control #1, Merck) to a final concentration of 45 nM and 400 ng of the respective rescue plasmid with 12 µL of Liopfectamine 2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... integrity was inspected on 1% agarose gel in TAE buffer pre-stained with GelRed nucleic acid stain (Merck).
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
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bioRxiv - Immunology 2024Quote: ... cells were briefly reactivated by addition of 10 ng/ml PMA and 1 μg/ml ionomycin (all Merck) for 1h prior to flow cytometric analysis ...
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bioRxiv - Immunology 2024Quote: ... Transformants were selected on vegetarian Yeast Extract-Peptone-Dextrose (YPD) medium supplemented with 1 M D-sorbitol (Merck) and 250 µg mL-1 Zeocin (InvivoGen) ...
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bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated with primary antibody rabbit anti-c-fos (1:1000, Merck Millipore, Burlington, United States) in TBS (5% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... to two of the biological replicates and 50 μg mL-1 puromycin dihydrochloride (Merck Life Science, Amsterdam, NL) to two other biological replicates.
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bioRxiv - Plant Biology 2023Quote: ... The seeds were then placed in a growth medium composed of 5 mM KNO3 (7757-79-1, Merck), 2 mM MgSO4 × 7H2O (10034-99-8 ...
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bioRxiv - Bioengineering 2023Quote: ... HUVEC or HUVEC/pericyte (ratio 10:1) solutions were mixed with thrombin (4U/mL, from bovine plasma, Merck) and 20 mg/mL fibrinogen (from bovine plasma ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transduced with 1 MOI of concentrated virus containing 8 ug/mL Polybrene (Merck, TR-1003-G). Two days later ...
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bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used are GAPDH (clone 6C5, #CB1001, 1:5000 dilution, RRID: AB_1285808, Merck Millipore, Burlington, MA, USA), GFP (ab290 ...
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bioRxiv - Plant Biology 2023Quote: ... plant material (c.a 50 mg) was extracted with 1 mL of 50% methanol/water (LC–MS grade, Merck). Then ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
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bioRxiv - Neuroscience 2023Quote: ... cryosections were incubated at RT for 1 h in darkness with the secondary antibody and Hoechst 33258 (Merck) as a nuclear counterstain ...
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bioRxiv - Molecular Biology 2023Quote: ... AA amyloidosis induction was performed by injections of 100 µl AEF (i.v.) and 200 µl sterile filtered 1 % silver nitrate solution (s.c.) (Merck). Repeated injections of silver nitrate were in accordance with the reference 59 ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample solution or marker (1 μl) was spotted onto a TLC plate (silica gel 60 F254, Merck). The TLC plates were developed appropriate times with 75% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... were homogenised in 250 mM sucrose / 20 mM triethanolamine with protease inhibitors (1% Merck Protease Inhibitor Cocktail III). The homogenate was cleared of large debris by centrifugation at 4000 g for 15 mins at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blot was performed according to standard procedures using the following antibodies: NELFE (1:500, Merck-Millipore, ABE48), Top1 (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... and then repeatedly washed with PBS-T before incubation in secondary antibodies for GRIN2D (Merck, MAB5578, 1:500) and B-actin (Merck ...
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bioRxiv - Immunology 2024Quote: ... Tissue was stained for 1 h with a mixture of Hoechst-34580 DNA dye (10 µg/mL; Merck) and Phalloidin Alexa 647 conjugate (1:400 ...
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bioRxiv - Pathology 2024Quote: Tear samples were collected successively from both eyes for 1 minute each using 1µL microcapillary glass tubes (Merck, Drummond Scientific ...
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bioRxiv - Immunology 2024Quote: ... before staining on ice in the dark for 1 h with mouse monoclonal anti-Puromycin-AF647 (Merck Millipore), diluted in the same solution ...
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bioRxiv - Molecular Biology 2024Quote: ... air-dried at RT for 30 min and fixed in Methanol:Acetic acid in a 3:1 ratio (Merck) at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... and then incubated with pre-equilibrated protein G agarose plus anti-FLAG M2 (F3165, Merck, 1 μl/IP), or 5 μl/IP anti-GFP (A-11122 ...
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bioRxiv - Immunology 2024Quote: ... dried immunoprecipitates were reconstituted in buffer (1% acetonitrile,0.1% TFA) and applied to a 10cm RP-18e 100-4.6 chromolith column (Merck) using an Ultimate 3000 HPLC equipped with UV monitor.
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Minced testes were dissociated in Dulbecco’s modified Eagle medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 1 mg/ml collagenase I (Merck) for 25 min at 37 °C and 0.05% trypsin-EDTA (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed at room temperature for 1 hour followed by several washing steps and subsequent embedding with Mowiol optionally containing DAPI (#1.24653; Merck).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... cell culture supernatant of HUVECs was concentrated to 1 mL using Amicon-Ultra 15 Centrifugal Filter Units (UFC901096, Merck) and incubated with 0.5 µg of Alexa 488 labeled anti-CD9 ...
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bioRxiv - Molecular Biology 2021Quote: ... The enzymes were concentrated to 10-19.5 mg ml−1 with Amicon Ultra 10k and 30k MWCO centrifugal filters (Merck), flash frozen in liquid nitrogen and stored directly at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 6 times for 10min with 1ml wash buffer (10mM HEPES ph 7.3, 150mM NaCl, MgCl2, 10% glycerol, 1% Empigen BB Detergent (Merck), 1x PI) ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal (RP) against phosphorylated Tyr 1077 ObRb (pTyr1077ObR; 1:500, cat# 07-1317, Merck Millipore, Burlington, Vermont, USA), GP against phosphorylated Tyr 1138 ObRb (pTyr1138ObRb ...
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bioRxiv - Microbiology 2019Quote: ... 1 μL of the reaction was spotted onto aluminium backed silica gel plates (5735 silica gel 60 F254, Merck) and dried before being subjected to Thin Layer Chromatography (TLC ...
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bioRxiv - Biophysics 2019Quote: ... we incubated clean glass cover slips (glued to 8-well LabTek chambers) with 30 µg ml−1 fibronectin (Merck) in 1 x PBS for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We next centrifuged the mix (1 min, 13,000 g) through a Durapore PVDF 0.22-μm filter (Merck Millipore, US). We injected the CRISPR-Cas9 solution into the male pronucleus of fertilized zygotes with a motor-driven manipulator-based microinjection stage ...
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bioRxiv - Cell Biology 2021Quote: ... the nuclear pellet was resuspended in 0.5 mL of ice-cold RIPA buffer supplied with 1 U/mL Benzonase (Merck), and incubated on a rotator for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4) (final concentration: 12mg/mL) containing 5 μL Benzonase (final concentration: 1 μL benzonase/mL (MERCK, 71205-3). After dissolving ...
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bioRxiv - Biophysics 2021Quote: ... Myosin II filaments were further diluted in KMEH to 200 nM and added to the actin filaments bound to the SLB by His6-Curly by replacing 1/10 of the sample buffer with the myosin II filament solution and supplemented with 0.1 mM Mg-ATP as well as a mix of 1 mM Trolox (cat. no. 648471, Merck), 2 mM protocatechuic acid (cat ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 1x 106 cells of each cell line were spin-infected for 1 h at 1.9 × 103 g (3000 rpm) in the presence of 10 μg/mL polybrene (Merck) to facilitate the infection ...
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Microbiology 2021Quote: ... which were fixed with methanol for 10 seconds and then stained with Giemsa (1:10 dilution in water; Merck) for 5-10 min prior to microscopy under 100 x magnification with oil.