Labshake search
Citations for Merck :
3851 - 3900 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
-
bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
-
bioRxiv - Biophysics 2023Quote: ... 5.5 µL of fibril sample was mixed with 0.5 µL of 1 mg/mL pronase protease (53702, Merck-Millipore) followed by adsorbing onto carbon-coated copper grids ...
-
bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
-
bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
-
bioRxiv - Microbiology 2024Quote: ... Mouse mAb anti-HA (clone HA-7) and mouse anti-tubulin (clone B5-1-12) were purchased from Merck. Mouse mAb anti-V5 (SV5-PK1 ...
-
bioRxiv - Physiology 2024Quote: ... Membrane was incubated overnight at 4°C with specific mouse monoclonal primary antibody anti-puromycin (Merck, MABE343, 1:1000) and after wash ...
-
bioRxiv - Plant Biology 2024Quote: ... The pellet was washed by resuspension in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.2% IGEPAL® [Merck]) and pelleted again in the magnetic rack ...
-
bioRxiv - Plant Biology 2024Quote: ... the buffer was replaced with complex IV staining solution [1 mg/mL DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Merck D5637), 1 mg/mL cytochrome c (Merck C7752)] in complex IV buffer) ...
-
bioRxiv - Cell Biology 2024Quote: ... The obtained residue was derivatized with N-trimethylsilylimidazole/ trimethylchlorosilane reagent (TMSI:TMCS, 99:1, v/v; 50uL; both from Merck) and pyridine (50uL ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibodies against γH2AX (the same antibody used for wester blotting) and 53BP1 (1:1000, MAB3802 Merck). Cells were washed and incubated with secondary anti-rabbit-Alexa Fluor 488 (1:500 ...
-
bioRxiv - Microbiology 2024Quote: “Swim” agar plates were made by pouring 0.3% LB agar into petri dishes with a 1-50 numbered grid sticker on them (Merck). Ciprofloxacin was added from a stock solution to each plate to a final concentration of either 0.015 μg mL-1 ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 worms from each RNAi treatment group were collected and cleaned in H2O before depositing in 1 μL of lysis buffer (0.25 mM EDTA (Merck), 5mM Tris pH 8.0 (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2024Quote: ... NPCs were thawed in STEMdif Neural Induction Medium + SMADi on 0.01% Poly-L-ornithine/10-20 µg ml−1 laminin-coated (MERCK) cell culture plates and differentiated using the STEMdiff Forebrain Neuron Differentiation Kit (Stem Cell Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.2 U μl−1 SUPERaseIn and 0.1% Triton X-100 in nuclease-free water) using a glass Dounce tissue grinder set (Merck). Samples were dissociated with 10-20 strokes of a loose pestle ‘A’ followed by 10 strokes of a tight pestle ‘B’ when tissue fragments remained ...
-
bioRxiv - Zoology 2024Quote: ... Fish were anesthetized with MS-222 (0.1 g l-1 of sea water) (Sigma-Aldrich; Merck group, Darmstadt, DE) before each sampling and injection procedure ...
-
bioRxiv - Immunology 2024Quote: ... Labeling efficiency was determined by ITLC using Whatman no.1 strips as solid phase and ethyl acetate (109623, Merck) as running buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell pellet from a 3 l culture was resuspended in 80 ml TE pH 7.5 buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA) supplemented with Complete EDTA-free protease inhibitor cocktail (Merck) and 2.5 U ml−1 benzonase until sample was homogenous ...
-
bioRxiv - Microbiology 2024Quote: ... The lipid film was hydrated with 1 ml of buffer A (10 mM HEPES pH 7.4, 150 mM KCl) containing 300 µM calcein (Merck), by vortexing in the presence of a 3 mm glass pearl ...
-
bioRxiv - Microbiology 2024Quote: ... Micrococcin P1 was produced as described previously and solubilized to a concentration of 10 mg/ml in dimethyl sulfoxide (Sigma-Aldrich).30 Nisin A was obtained as part of a commercial fermentate which was solubilized to a concentration of 1 mg/ml in a 0.05% acetic acid solution (Merck). AuresinePlus was solubilized to a stock concentration 1 mg/ml in a buffer containing 20 mM Tris-HCl pH 7.0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
-
bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
-
bioRxiv - Plant Biology 2024Quote: ... Both input and immunoprecipitated samples were separated by 12% SDS-PAGE and detected using anti-GFP (1:2000, Merck) and anti-Myc (1:2000 ...
-
bioRxiv - Neuroscience 2024Quote: ... DMSO (0.1%; D4540), L-glutamic acid (L-glutamate, 1 mM; G1251) and Sodium Propionate (5mM; P1880) were obtained from Merck Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... After heat induced antigen retrieval (Citrate buffer, pH6) of samples an anit-TSP1 antibody (1:400, Merck/Millipore MABT879) was diluted in blocking solution and incubated at 4°C overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... SB431542 and LDN193189 were added until day 7 when cultures were supplemented with 1 μM smoothened agonist (SAG; Merck) and 0.5 μM purmorphamine (Pur ...
-
bioRxiv - Biochemistry 2024Quote: The monomeric recombinant peptide was prepared by resuspending it in a solution of 1% NH4OH (Merck Cat. No. 221228) in PBS buffer at a concentration of 1 mg/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... depending on experiment (usually 104 cells/cm2 for three-day differentiation) in medium supplemented with 1 μM RA (Merck) and deprived of LIF for indicated periods of time ...
-
bioRxiv - Cell Biology 2024Quote: ... One of them was an anti-phospho-histone γH2A.X antibody (Cell Signalling Technology, Danvers, United State; 1:250) and the second was anti-NeuN (Merck Millipore ...
-
bioRxiv - Cell Biology 2024Quote: ... and the cell pellets (1.5 x 106 cells) were lysed with 150 µL of 1× RIPA buffer (Merck Millipore), kept on ice ...
-
bioRxiv - Genetics 2024Quote: ... Then 1 μl of the digest RNA sample was spotted on cellulose F TLC plates (20 × 20 cm, Merck) and run for the first dimension in a solvent system of iso-butyric acid:NH4OH(28-32% ...
-
bioRxiv - Cell Biology 2024Quote: ... Fabs were concentrated up to more than 1 mg/mL using 10 k cut-off filters (Amicon Ultra-0.5 10 k; Merck), according to the manufacturer’s instruction.
-
bioRxiv - Developmental Biology 2024Quote: ... followed by overnight incubation at 4°C with 250µl of the anti-DIG antibody (Merck #11093274910 diluted 1:2000) and other necessary primary antibodies (when additional immunostaining was needed ...
-
bioRxiv - Microbiology 2020Quote: ... purified ParB was concentrated by centrifugation in an Amicon Ultra-15 3-kDa cut-off spin filters (Merck) before being loaded into a Superdex 75 gel filtration column (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mo and PN were then washed in DMEM and placed in 3 μm Boyden chambers (Merck Millipore, France), at the concentration of 0.5 x 106 cells/chamber in 200 µl DMEM ...
-
bioRxiv - Microbiology 2021Quote: ... purified ParBΔCTD was concentrated by centrifugation in an Amicon Ultra-15 3-kDa cut-off spin filters (Merck) before being loaded into a Superdex-200 gel filtration column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... and HPLC separation with a ZIC®-cHILIC column (3 µm, 100 Å, 150 mm × 2.1 mm, Merck) and a similar guard column (20 mm × 2.1 mm ...
-
bioRxiv - Physiology 2020Quote: ... at 4°C for 60 min using an Amicon Ultra-4 3 kDa centrifugal filter device (Merck Millipore). The 50 μL retentate was the final volume of concentrated EVs ...
-
bioRxiv - Systems Biology 2020Quote: Wnt signal inhibitor (CK) stock (2.4–3 mM): CKI-7 dihydrochloride (#C0742-5MG, Merck & Co., Inc., NJ, USA) diluted in distilled water (Otsuka Pharmaceutical Factory ...
-
bioRxiv - Cell Biology 2022Quote: ... The corresponding GST fusion protein was added to a final concentration of 15 µg/ml in incubation buffer (10 mM Tris pH 8.0, 150 mM NaCl, 0.1 % Tween-20, 3 % BSA (fatty acid free, Merck)) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 3 µL of sample was carefully spotted onto pre-washed PEI-cellulose TLC plates (Merck Millipore, #105725). The TLC plate was eluted in developing buffer containing 10 mM NH4Cl and 5% acetic acid for 100-140 min ...
-
bioRxiv - Neuroscience 2020Quote: ... the eluate was up-concentrated to 3 ml using an Amicon Ultra 3000 MW centrifugal filter unit (Merck). Concentration assessment with a NanoDrop (∊ = 2980 ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfections of DNA plasmids were carried out with the NanoJuice Transfection Kit (71900-3, Merck, Kenilworth, NJ, USA) or the Avalanche-Everyday Transfection Reagent (EZT-EVDY-1 ...
-
bioRxiv - Cell Biology 2022Quote: ... expanding cells were transferred to erythroid differentiation medium (Iscove’s medium with stable glutamine [Merck] containing 3% (v/v) AB serum [Merck] ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
-
bioRxiv - Microbiology 2023Quote: Supernatants showing biological activity were concentrated using Amicon Ultra-0.5 centrifugal filters (3 kDa) (Merck KGaA, Darmstadt, Germany) as follows ...
-
bioRxiv - Genetics 2023Quote: ... Supernatant was then transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, no. UFC500396) and centrifuged for 45 min at 4 °C ...