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Citations for Merck :
2751 - 2798 of 2798 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1.5E05 cells were seeded onto a T25 Matrigel coated flask in StemFlex medium supplemented with 10 µM rock inhibitor (Merck Millipore, Y-27632). Medium was refreshed the following day without rock inhibitor and lived cells were sent over to Cell Line Genetics when 20-30% confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Microbiology 2022Quote: The His6-eVP40-pET46 expression vector was a kind gift from Erica Ollmann Saphire (La Jolla Institute for Immunology) and was expressed and grown in RosettaTM 2 BL21 DE3 competent cells (Merck Millipore, Billerica MA). The pet28a-His6-Lact C2 bacterial expression plasmid was a kind gift from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were grown in M9 medium (Green et al., 2012; Meyer and Schlegel, 1983) containing (15NH4)2SO4 (Sigma/Merck KGaA, Darmstadt, Germany) or (15NH4)2SO4 and 13C-D-glucose (Euriso-Top GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... the bacteria were inoculated into the same medium (0.2% v/v) and the cell density was monitored using a Guava EasyCyte flow cytometer before harvesting (Merck Millipore, Billerica, MA, USA). Cell pellets were harvested from 10 ml culture by centrifugation which were stored at −80°C prior to lipid extraction.
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Microbiology 2021Quote: ... Five milliliters of each standard solution was placed in a flat-bottom test tube for spectrophotometer analysis (Spectroquant Empty cells 16 mm; Merck KGaA, Darmstadt, Germany), which was also used for incubation ...
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bioRxiv - Cancer Biology 2023Quote: 1000 freshly sorted GFP+ and GFP- MM13 and MM27 cells were grown as hanging drops in IMDM complete medium (Sigma Aldrich-Merck, Cat#I3390) for 72 h at 37°C in normal (20%O2 ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020). For biochemical experiments ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Neuroscience 2023Quote: ... filtered to remove cell debris and particles were concentrated (30-fold) via low-speed centrifugation (Amicon Ultra-15, Ultracel-100; Merck Millipore; Cat# UFC9100) before aliquoting and storage at −70°C ...
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bioRxiv - Neuroscience 2023Quote: Cerebellar organoids were generated as previously described (Silva et al 2020) with some alterations: 80-90% confluent iPSCs were dissociated into single cells using Accutase (Merck, Cat. no. A6964), and 4,500 cells were seeded per well of 96 well V-bottom low adhesion plates (S-bio ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with freshly made SA-β-gal buffer containing 25 mM Na2HPO4 (Merck Life science), 7.4 mM Citric Acid (Merck Life science) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Neuroscience 2024Quote: ... The clarified supernatants were diluted by addition of 25% vol fresh medium and used to transduce HEK293T cells with Polybrene (Merck, #TR-1003-G) at a final concentration of 10 µg/mL for 72 h.
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y human neuroblastoma cell line was cultured at 5% CO2 in DMEM medium at high glucose (D6429, Merck Life Science, Milan, Italy), containing 10% (v/v ...
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bioRxiv - Genomics 2024Quote: Cerebellar organoids were generated as previously described84 with some modifications: 80-90% confluent iPSCs were dissociated into single cells using Accutase (Merck, Cat. no. A6964), and 4,500 cells were seeded per well of 96 well V-bottom low adhesion plates (S-bio ...
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bioRxiv - Bioengineering 2024Quote: ... cells were cultured under standard cell culture conditions in FBS-free RPMI-1640 cell culture medium supplemented with 300 nM phorbol 12-myristate 13-acetate (PMA; Merck KGaA, Darmstadt, Germany) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Cell Biology 2024Quote: ... HL-60 cells differentiated into macrophages after one week of supplementing with 100 nM vitamin D (VD) analog EB1082 (Merck/Sigma-Aldrich, Milan, Italy) (James et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cell Biology 2022Quote: The cellular health of Eca EPCs Non-OB and Eca EPCs OB was evaluated using Guava® Muse® Cell Analyser (Sigma-Aldrich/Merck, Poznan, Poland). The metabolic activity of cells was determined based on mitochondrial membrane potential using Muse™ Mitopotential Assay Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies were added to each aliquot (equivalent to 100 ml of cell culture): 6μl anti- H3K56ac for the mid-S time point (Merck-Millipore, 07-677-IS (lot# 266732) or 10 μl anti- H3K56ac for the early-S time point (Active Motif ...
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bioRxiv - Cell Biology 2024Quote: ... rat neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL poly-D-lysine (PDL, Merck/Sigma-Aldrich #A-003-E). Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: The pancreatic β cell line INS-1E was cultured in a humidified chamber with 5% CO2 in RPMI-1640 medium (Merck, Sigma Aldrish, Darmstadt-Germany) at 11.1 mM glucose supplemented with 5% (vol./vol.) ...
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bioRxiv - Cell Biology 2024Quote: ... reducing RAD2l levels about 40% relative to our CTCF-EGFP line (using Simple Western of asynchronous cell lysates, RAD2l detected via anti-RAD2l antibody (0S-908, Merck Millipore, l:S0, Suppl. Fig. 4G). 0verexpression of RAD2l rescued this effect ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C overnight with primary antibodies (HIF1α, 1:1000, Novus Biologicals, NB100-134; HIF2α, 1:1000, Cell Signaling Technology, 7096; β-Actin, 1:5000, Merck, A1978) in 0.1% skimmed milk in TBST buffer (blocking buffer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were dissociated by mincing and enzymatic digestion of the extracellular matrix using 500 µg/ml collagenase type 1a (Merck Life Sciences UK Ltd, Gillingham, U.K) and 100 µg/ml DNAse I (Lorne Laboratories Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Microbiology 2020Quote: ... as previously described (details in Supplementary materials).13–16 Organoid-derived monolayers (ODMs) were prepared in Matrigel-coated transwell cell culture inserts (Merck Millipore, 0.6 cm2, 0.4 μm pores). For this ...
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Hydrothermally treated yeast cell wall suppresses the growth of MCF7 cells under isolated conditionsbioRxiv - Biochemistry 2024Quote: ... Cell counting was performed automatically by Guava® easyCyteTM 5HT and the analyses were provided plots separating live and dead cells by the Guava® ViaCount™ Software Module (Merck Millipore, Burlington MA USA).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and incubated with the primary antibody (cofilin, Cell Signaling, cat. 5175S, 1:3000; HA-Tag, Santa Cruz, sc-7392, 1:1000; GAPDH, Merck Millipor, No. AB2302, 1:2000) for 12 hours ...