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Citations for Merck :
2601 - 2650 of 2798 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoid cells were cultured in self-renewing medium with ROCKi for 8 days before Dox (Doxycycline hyclate, 2 µg/ml, Merck, D9891) and TMP (Trimethoprim ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 100μM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP; Merck Life Sciences, Gillingham, UK) to activate the P2RX7 ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed and stained according to the procedure described above using primary antibodies against NESTIN (Merck, ABD69, 1:300 dilution) and TUBB3 (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of ZsGreen1-expressing cells (%ZsGreen) and ZsGreen1 mean fluorescence intensity (MFI) of each sample were analyzed using Guava ExpressPlus software (Merck Millipore) and MFI was expressed as fluorescence arbitrary units (FAU) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... 72 hours conditioned-media from cancer cells was collected and filtered with a 0.45 μm filter (Millex, Merck Millipore, Massachusetts, USA) before adding to fibroblasts ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were dissolved in 1 ml of PBS and nuclei were stained with propidium iodide (PI) (Merck Millipore, Burlington, MA, USA) and analyzed using a flow cytometer (Guava PCA) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50% confluent cells were plated and allowed to adhere for at least 24 hours before treatment with 1 μM 4OH-TAM (Merck, H7904) or with identical volume of EtOH for the indicated time ...
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bioRxiv - Systems Biology 2022Quote: Yeast cell culture pellets were resuspended in lysis buffer composed of 100 mM triethylammonium bicarbonate (TEAB) (Merck Sigma, Cat. No. T7408) containing 0.1% ...
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bioRxiv - Molecular Biology 2022Quote: Antibody-bead mixture was washed twice in iCLIP lysis buffer to remove unbound antibody and RNase-treated cell lysates were added alongside cOmplete EDTA-free Protease Inhibitor Cocktail (Merck, #11836170001) and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Neuroscience 2021Quote: ... These slices were explanted onto Millicell cell culture inserts (pore size 0.4 µm, diameter 30 mm, Merck Millipore Inc., cat# PICMORG50). Membrane inserts were placed into a six-well plate with 1 mL of culture medium ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... USA) cells were transduced with lentiviral particles at 70% confluency in the presence of polybrene (TR-1003-G, Merck, Darmstadt, Germany). 24 h after transduction virus containing medium was replaced byG-418 sulfate (#M3118.0050 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Neuroscience 2022Quote: ... To fix the cells they were washed once with warm PBS and incubated for 20 min at 37°C with 4 % paraformaldehyde (Merck Millipore). After washing ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 min at 37 °C and washed with 5% FBS (EmbryoMax® ES Cell Qualified FBS, ES-009-B, Merck) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... human iPSC cells were detached using Accutase for 5min at 37 °C and followed dissociated into single cells with phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA). Cells were centrifuged at 300 rpm for 5 min and the pellet was resuspended in hESC medium (20% knockout serum replacement ...
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bioRxiv - Cell Biology 2020Quote: ... Staining was performed on fixed cells using an antibody directed against the hCoV-OC43 nucleoprotein as a primary antibody (Merck #MAB9013) followed by a horse radish peroxidase labeled detection antibody (Thermo Scientific #31430 ...
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bioRxiv - Microbiology 2020Quote: ... and the proportion of EGFP-positive cells was determined every day by flow cytometry using a Guava EasyCyte Flow Cytometer (Merck Millipore). The difference of the percentage of EGFP negative cells between induced and non-induced conditions were plotted as a function of time of growth ...
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bioRxiv - Immunology 2021Quote: ... 20 μl of this cell/collagen mixture was added in one well of pre-cooled Corning™ 96-Well half-area plate (Merck) and the plate was centrifuged ...
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bioRxiv - Immunology 2022Quote: Cells were lysed by addition of a mild lysis buffer containing 1% octyl-β,D-glucopyranoside (#O9882-500MG, Sigma-Aldrich, Merck), 0.25% sodium deoxycholate (#1065040250 ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Synthetic Biology 2022Quote: Cells were grown in CellASIC ONIX microfluidic plates for haploid yeast cells in media controlled by the ONIX2 microfluidics system (Merck, Germany). Details regarding the composition of the media used for different promoter induction experiments are given in Supplementary Note 1.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cancer Biology 2022Quote: ... HIC1 KO cells were seeded on coverslips and DNA damage was induced by incubation with 10 μM etoposide (Sigma-Aldrich/Merck) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... removed and faux adherent cells from the lower membrane washed into the bottom well before migrated cells were counted using a Guava EasyCyte 8HT Flow cytometer (Merck Millipore). Data was then presented as the total number of migrated cells or relative to vehicle controls (% control).
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bioRxiv - Genetics 2022Quote: In vitro differentiated 18 d erythroid cells stained with Hoechst 33342 were resuspended with 150 μl DPBS for analysis with Imagestream X Mark II (Merck Millipore). Well-focused Hoechst-negative single cells were gated for circularity analysis with IDEAS software ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with a BG4-Flag antibody (1:100 diluted in 0.5% goat serum + PBST, MABE917, Merck, Darmstadt, Germany) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the heart was removed and cells were dissociated and filtered through a 70 µm filter after which BSA (Merck Life Science) was added to a final concentration of 4% to inactivate the enzyme ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The pNGS2-N12-BC libraries were prepared by purification from the harvested cells by using either GenElute Plasmid Miniprep (Merck, Germany) or NucleoBond Xtra Midi (TaKaRa Bio Inc. ...
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bioRxiv - Genetics 2023Quote: Wild type and PREPL KO HEK293T cells were grown on glass coverslips coated with Poly-D-Lysine (Merck A-003-E) and fixed with 4% formaldehyde ...
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bioRxiv - Genomics 2024Quote: ... 8 x 106 HeLa-Cas9-p65-mNeonGreen cells 10 were transduced with 1 ml lentivirus library and 10 μg/ml polybrene (Merck Millipore). After one day ...
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... cells were split into either 6 well-plates (300000 cells per well) coated with 1 mg/ml Poly-L- Lysine (PLL, Merck, P9155) for western blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X Protease inhibitors and 1X Phosphatase inhibitors (PPI)] plus 2μL per sample Pierce Universal Nuclease for Cell Lysis and 1μL per sample Benzonase® Nuclease (Merck, E1014-25KU) for 30min at 4°C on wheel with vigorous pipetting every 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...