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Citations for Merck :
2501 - 2550 of 2798 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... organoids generated from the H9 doxycycline-inducible ZEB2 (HumiZEB2) cell line were treated with 1.7 μg/ml doxycycline (Merck, PHR1145) during the first 5 days of the protocol.
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bioRxiv - Cell Biology 2020Quote: ... pBADthio-LmaCK1.2ΔC43- V5-His6 and pBADthio-LmaCK1.2ΔN7-V5-His6 were sequenced and transformed in Escherichia coli Rosetta (DE3) pLysS Competent Cells (Merck Cat# 70956) for bacterial expression.
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bioRxiv - Biochemistry 2021Quote: ... Transient transfection of U2OS and HepG2 cells with plasmid DNA was carried out using GeneJuice (Merck Millipore, cat. number 70967) according to manufacturer’s instruction and keeping GeneJuice to DNA ratio of 3:1 (volume in μl ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Microbiology 2021Quote: ... CTR1 KO cells were generated by co-transfecting 293T cells with the pX458 vector carrying the Cas9 protease fused to GFP and a Sanger lentiviral CRISPR vector (Merck) carrying the BFP and a sgRNA against human CTR1 under the control of the U6 promoter (sgRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... iPSCs were maintained according to [71] in 60-mm Petri dishes (0,6 × 105 cells total per dish) coated with 0.1% gelatin (G2625, Merck, Darmastadt, Germany) in Glasgow’s Minimum Essential Medium (GMEM ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Neuroscience 2022Quote: ... microglia were isolated and cultured for 3 days as above before cells were lysed in RIPA buffer (60 μL/well, Merck). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with MISSION® SiRNA oligomers or MISSION® siRNA Universal Negative Control #1 (Sigma Aldrich, Merck, USA) at a final concentration 20 nM ...
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bioRxiv - Immunology 2022Quote: Decidual cells were isolated as described above and incubated in the presence of fluorescence-labeled latex beads (50 beads per cell; latex beads, 1 µm, carboxylate-modified polystyrene, fluorescent yellow-green, Merck) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Infection and selection of stably infected cells were done in the presence of the appropriate concentration of puromycin (Merck-Aldrich), titrated by using the minimum antibiotic concentration sufficient to kill untransfected cells ...
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bioRxiv - Neuroscience 2022Quote: ... cells were replated onto coverglass (8×104 per well) coated with 50 µg/ml Poly-D-Lysine (PDL, Merck, USA) for imaging experiments.
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bioRxiv - Bioengineering 2022Quote: ... were rinsed with PBS and incubated in 500 μL of cell lysis solution containing 0.2% (v/v) Triton-x-100 (Merck, 1.08603.1000) in 5 mM MgCl2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Genetics 2022Quote: ... Transient transfection was carried out in HEK293T cells transfected with 1 μg of plasmid DNA in the presence of X-tremeGene9 DNA transfection reagent (#6365809001, Merck), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Parasitemias were calculated from day 3 post-infection by counting infected red blood cells in blood smears stained with Hemacolor (Merck).
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bioRxiv - Neuroscience 2022Quote: Tissues or cells were homogenized in Pierce IP Lysis buffer supplemented with protease inhibitor cocktail 3 (Merck Millipore, Darmstadt, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Lgr5+ cells in the mice were ablated with a single intraperitoneal dose of Diphtheria Toxin (DT) (Cat # 322326-1mg, Merck) in saline (50 µg kg−1).
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bioRxiv - Cell Biology 2022Quote: Yeast cells were grown to mid-log-phase on SD-Leu media and analyzed on a CellStream analyzer instrument (Merck) using 488 nm and 561 nm lasers for capturing the fluorescence emission of GFP and Cherry ...
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bioRxiv - Neuroscience 2024Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4°C with primary antibodies (α-TOM20 Santa Cruz # sc-11415; α-STIM1 Merck WH0006786M1) diluted (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were starved (0% serum) for 24h and then treated for 96h with 5mM Metformin (Merck, cat. nr M0605000) or 2ng/ml TGF-β1 (Peprotech cat nr 10021) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cytospins were prepared by spinning 30.000 cells per slide and stained with May-Grünwald-Giemsa staining (Merck KGaA, Darmstadt, Germany). 2*200 cells were identified by morphology and counted at 20x magnification (Olympus BX51) ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck). Cell vials were stored in a liquid nitrogen tank.
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... the culture medium of the cells was replaced at day 3 with differentiation medium containing 10 mM PEG950 (Merck; P3515).
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture supernatants from 4950T or 170162T cells were concentrated 10x using Amicon centrifugal filtration units with 100 kDa cutoff filter (Merck). Concentrated supernatant or glycodelin A isolated from amniotic fluid was added to the lectin coupled beads and incubated overnight on an overhead shaker at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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bioRxiv - Molecular Biology 2024Quote: ... Cumulus cells were removed by briefly culturing the zygotes in potassium simplex optimization medium (KSOM) (catalog no. MR-101-D, Merck) supplemented with 0.3 µg.µL-1 hyaluronidase (catalog no ...
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bioRxiv - Genomics 2023Quote: ... Cell lysis was achieved by pre-incubating bacterial colonies in Phosphate-buffered saline (PBS) containing 100 μg/mL lysostaphin (Merck) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were plated per well and were further cultured in the presence or absence of 5 µM oligomycin A (Merck) at 37 °C in a humidified chamber containing 5% CO2 and 1% O2 for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The explants were allowed to attach to the feeder layer before epithelial cell medium (ECM) containing Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DNEM-F12, #D8062, Merck) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Cell Biology 2024Quote: ... Forty eight hours of post-transfection the viral supernatants were passed through 0.45 μm syringe and the diluted viruses were used to infect cells in growth media supplement with 8 μg/ml of fresh polybrene (Merck) for 8 hr for three consecutive times ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in T cell medium for 30 min at 37°C without any metabolic inhibitors or in presence of 1 μM oligomycin (Merck), 100 mM 2DG (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Neuroscience 2023Quote: ... coated dishes at low confluency (5,000–10,000 cells/cm2) in MEF-conditioned hES medium supplemented with 10 μM ROCK inhibitor (Y-27632; Merck, Cat#688000). On DIV0 of the differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... Product number E4250) (1 mg/ml) to contract pigment cells alongside 0.01% Tricaine (Ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich (Merck) Product number E10521 ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Immunology 2023Quote: ... the streptavidin tetramer mixture (containing D4, A1D3, A2D2, A3D1 and A4) was concentrated in an ultrafiltration cell (10 kDa cut off, Merck); mSav (A106C ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...