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Citations for Merck :
2651 - 2677 of 2677 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the cells and supernatants were harvested and purified using chloroform and were condensed using Amicon Ultra-4 Centrifugal Filter Devices (100K MWCO; Merck Millipore, Billerica, MA).
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 3,000 × g at 4°C and filtered under sterile conditions using a 0.22 μm filter (Millex-GV, PVDF; Merck/Millipore Watford, Hertfordshire, UK), and is referred to as CM-OAT1.
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bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Microbiology 2022Quote: Non-infected and MCMV-infected mice were orally gavaged with 150 μl of 100 mg/ml 4 kDa FITC-dextran-labeled dextran beads (Sigma-Aldrich/Merck, Darmstadt, Germany) in PBS 4 hours prior to sacrifice ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Microbiology 2023Quote: ... Residues in solution were pelleted by centrifugation at 12000Xg for 30 minutes at 4℃ and soluble fraction was filtered through 0.22-micron filters (Merck Millipore Ltd, Cork, IRL). Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Genomics 2021Quote: ... The lysate was incubated for 2 hours at 4°C with 180 µl of anti-flag agarose beads (50 % slurry, ANTI-FLAG® M2 Affinity Gel, Merck, Kenilworth, NJ, USA), prewashed 3 × with 1 ml isotonic wash buffer (IsoWB ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HPLC separations were performed on a Waters 2695 separations module equipped with a Waters 2996 photodiode array detector and HPLC analysis was carried out using a LiChrospher® 100 RP-18 column (4 mm × 250 mm, 5 μm) (Merck, Kenilworth, NJ, USA). The mobile phase consisted of two solvent reservoirs ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473; H3K9me2 Abcam ab1220; H3K27me3 – Acive Motif 39155; H3 – Merck 07-10254; PolII – Abcam ab817) before incubation with the sheared chromatin ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed two times with 1X Phosphate Buffer (30 mM Na2HPO4·12H2O [Merck, 10039-32-4]; 33 mM NaH2PO4 ·2H2O [Merck, 13472-35-0]; pH 7.4) and were dried on Superfrost (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf tissues were cut into 5 mm-long x 2 mm-wide strips along the leaf proximodistal axis in a drop of water using a razorblade and immediately immersed in room temperature 4% w/v paraformaldehyde (pH6.9) (Merck Life Science UK Ltd., Gillingham, UK). Tissue was immediately placed in darkness and fixed at 4°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...