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Citations for Merck :
2251 - 2300 of 2677 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were then fixed and permeabilized using a combination of 4% paraformaldehyde (Sigma-Aldrich/Merck) and 0.1% Triton X-100 (Sigma-Aldrich/Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated at 3,500 rpm and 4°C using Amicon Ultra centrifugal units (Merck).
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bioRxiv - Neuroscience 2023Quote: ... and transcardially perfused with 0.9% NaCl followed by 4% paraformaldehyde (PFA, Sigma, Merck, Germany, P6148). The brains were harvested and post-fixed overnight in 4% PFA at 4°C ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with 0.1% saponin in PBS and incubated with 4 μg/ml Hoechst 33342 (Merck) for 10 min at room temp ...
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bioRxiv - Systems Biology 2024Quote: ... UK) and separated by gel electrophoresis (7.5% acrylamide, 4 mg/ml porcine gelatin; Merck, UK). SDS was removed by washing in 50 mM Tris.HCl ...
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bioRxiv - Biochemistry 2024Quote: ... We then stimulated the cells for 4 h with 100 ng/ml PMA (524400; Merck) and 1 µg/ml ionomycin (407952 ...
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bioRxiv - Biochemistry 2024Quote: ... and blocked overnight at 4°C with 1% bovine serum albumin (BSA, Merck, Cat# 7906) in PBS (200 µl per well) ...
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bioRxiv - Cell Biology 2024Quote: Cultured cells for 24 hours of incubation were fixed with 4% paraformaldehyde (PFA) (#104005, Merck) in Hank’s balanced salt solution containing 1 mM Ca2+ and Mg2+ ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Cell Biology 2024Quote: ... the qRT-PCR was performed with custom designed Thermo Fisher probes (Supplemenatary table 6) using Universal Sybr Green Master Rox (Merck Life Sciences, 4913850001) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cell Biology 2023Quote: Differentiation into the three germ layers was performed in suspension culture in Corning® Costar® Ultra-Low Attachment 6-well-plates (Merck, Darmstadt, Germany). Prior to seeding ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: Blocks of cells or tissue were incubated overnight in 2.3M sucrose at 4°C (Merck, K17687153) in 0.1M phosphate buffer ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were detached in a similar manner: 4 mg/mL lidocaine hydrochloride (L5647; Merck KGaA) were solved in Versene Solution freshly for each detachment ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were washed 4 times in PBS and then embedded using Aquatex (Merck Millipore, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... the medium was removed and cells were fixed for 10 min in 4% formaldehyde solution (Merck). The fixation solution was removed and the cells were washed twice with nuclease-free H2O (nfH2O) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.1% SDS (Merck Millipore, Burlington, MA). Primary antibodies were incubated for 1 h at RT a humid chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of each sample were concentrated with Amicon ultra Centrifugal filters (Cat no. UFC510024, Merck). Samples were centrifuged (14 000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with Amicon Ultra-4 Centrifugal Filter Unit (100 kDa cut-off, Merck Millipore, Cat#UFC810024) to ~1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting ultrafiltrate (15min 14000g 15°C) was acidified with 20 µl 4% formic acid (Merck) in dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...
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bioRxiv - Developmental Biology 2021Quote: ... iPSC-derived mesodermal cells were fed with basal medium supplemented with 4 μM IWR-1 (Merck) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... Fractions of interest were concentrated using 10 kDa cut-off Amicon Ultra-4 concentrators (Merck Millipore) before loading on a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... 400 μl cell lysate were incubated with 4 μl HTT MAB2166 rabbit primary antibody (Merck, #MAB2166) for 2 hours at 4° C on a rotating device ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...
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bioRxiv - Immunology 2020Quote: ... naïve T cells were freshly isolated and subsequently cultured for 4/5 days in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... BJ-5at fibroblasts were kept in a 4:1 mixture of the Dulbecco’s Medium (Merck, #D6429) and Medium 199 (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: Fresh embryos and postnatal pituitaries were fixed with 4% w/v paraformaldehyde (PFA, P6148, Merck-SIGMA) in Phosphate-Buffered Saline (PBS ...
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bioRxiv - Genomics 2022Quote: ... the membranes were probed (overnight at 4°C) with rabbit polyclonal anti-tyrosine hydroxylase (Merck, AB152) diluted in TBS/0.2% Tween/2% milk ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were decapitated and the ONs were rapidly removed and fixed with 4% paraformaldehyde (PFA – Merck) diluted in PBS for 16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... under gentle agitation for 24 to 36 h in a fixative mixture of 4% acrylamide (Merck), 4% paraformaldehyde and 0.25% VA-044 thermal initiator (Fujifilm ...
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bioRxiv - Neuroscience 2023Quote: ... with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin19 ...
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bioRxiv - Neuroscience 2022Quote: ... coated coverslips and fixated with 4% Paraformaldehyde (PFA; EMC 15710) and 0.2% Glutaraldehyde (Merck Millipore 104239) for 15 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... Amicon Ultra-4 centrifugal filters with 100 kDa molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 μM Nalgene® syringe filter units (sterile ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested DIP material was clarified (3000 × g, 10 mins and 4 °C) and sucrose (Merck, #84097) was added at a final concentration of 4 % ...
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bioRxiv - Cell Biology 2023Quote: The eluate was concentrated using an Amicon Ultra-4 concentrator (Merck Millipore, 10 kDa cut-off), and incubated overnight at 4°C with TEV and 3C proteases to remove the C-terminal StrepTagII and C-terminal 6xHis tag from the HAUS2 and HAUS1 subunits respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Amicon Ultra-4 centrifugal filters with 100,000 Da molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 µM Nalgene® syringe filter units (sterile ...
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bioRxiv - Microbiology 2023Quote: SDS gel electrophoresis was performed using pre-cast mPAGE™ 4-20% bis-tris gels (Merck) and 20 µl of PS extracts were mixed in Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: The cells in 96-well plates were fixed with 4% paraformaldehyde (PFA, Merck, Cat. no. P6148) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing STAR635P-conjugated mSAv were concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... Monomeric STAR635P-labeled mSAv-DNA was concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing STAR635P-conjugated mSAV were concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and conjugated to trans-Cyclooctene-PEG3-Maleimide (TCO-PEG3-Maleimide ...
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bioRxiv - Cancer Biology 2021Quote: ... Organoids were washed once with ice-cold PBS and were fixed by 4% PFA (#1004965000, Merck Millipore) for 20 min at RT and stored in PBS at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Cells were fixed with 4% paraformaldehyde (Intron, Seongnam, Republic of Korea) and 100% methanol (Merck, Darmstadt, Germany). The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon ...