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Citations for Merck :
2501 - 2550 of 2677 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Neuroscience 2024Quote: ... or Tris-HCl buffered saline (TBS) and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917, 1:500 dilution; anti-GluA1, Merck-Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... were mixed at a ratio of 4:1 based on monomer and injected onto a gel-filtration column (Superdex 200, 16/600, Merck) equilibrated in 20 mM HEPES buffer (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... A 6:4 ratio of 0.1 % Oil-Red-O in isopropanol to 4 mL distilled water was prepared and passed through a 0.22 □m syringe filter (Merck: Z260479) before use ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Biochemistry 2024Quote: ... leaf punches were taken with a 4-mm biopsy punch and floated in 100 μL of H2O with 20 μM coelenterazine (Merck), using individual cells of a white 96-well white bottom plate (Greiner F-Boden ...
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bioRxiv - Molecular Biology 2023Quote: ... 10,000 cells were seeded in 12-well dishes and co-infected the next day with 4EBP1 and 4EBP2 shRNA viruses in medium supplemented with 4 µg/ml polybrene (Merck). Cells were grown until confluency and reseeded into 6-well dishes prior to addition of 1 µg/ml puromycin (InvivoGen ...
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bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
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bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin- labeled probes and biotin-labeled probes were detected with 4 µg/mL of anti-digoxigenin Fab fragments conjugated with FITC (Merck) and 1/1,000 diluted streptavidin conjugated with DyLight 549 (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled fractions were diluted with 2 vols of KPEM buffer to achieve a final concentration of 125 mM KCl and then incubated for 1 hr with 4 mL anti-FLAG monoclonal antibody conjugated resin (M2 agarose, Merck) using rolling ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at low density following incubation with DNA or siRNA 24h prior to fixation with 4% paraformaldehyde (PFA, Merck) for 15min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Molecular Biology 2024Quote: Candidate genes were amplified from Drosophila melanogaster ovary cDNA library using KOD Hot Start DNA Polymerase (Merck Millipore, 71086-4) and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation (20.000xg, 15 min, 4°C) and protein concentration was determined by Direct Detect® spectrometer (Merck). The proteasome activity was measured using a 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... Mouse peritoneal macrophages (MPMs) were isolated from mice 4 d after the intraperitoneal injection of thioglycollate (Merck & Co.; Kenilworth, NJ, USA) as described previously(Zhang et al. ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C with a SW28 rotor in a Beckman XL-70 centrifuge or concentrated using an Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore). Pellets were resuspended in 500 µL PBS ...
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing monomeric I-Ek/MCC(ANP)-biotin complexes were concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK), snap frozen in liquid nitrogen and stored in 1x PBS at −80 °C ...
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bioRxiv - Biophysics 2020Quote: ... dialyzed against Milli-Q water and concentrated to 4 mg ml-1 using Amicon Ultra centrifugal filter units with Ultracel-50K (Merck Millipore). UMODe filaments were obtained by limited digestion of UMODfl with elastase (Jovine et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... a blocker of the sarco/endoplasmic reticulum Ca2+-ATPase pump (SERCA) and Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP, Merck-Sigma-Aldrich), a protonophoric uncoupler of mitochondrial oxidative phosphorylation that depolarizes the mitochondrial membrane and leads to the organelle’s release of calcium ...
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bioRxiv - Cell Biology 2021Quote: ... 25 min) and alkylated (20 mM iodoacetamide, 30 min, room temperature) and samples concentrated with Amicon Ultra-4 columns (Merck-Millipore), After protein precipitation with methanol/chloroform ...
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bioRxiv - Microbiology 2020Quote: Feces were hydrated in 500 µL sterile Milli-Q water overnight at 4 °C and then mixed with 500 µL of absolute ethanol (Merck, USA) for 60 min at RT ...
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bioRxiv - Plant Biology 2020Quote: ... on a LiChroCART® column (LiChrospher 100 RP-18, 125 x 4 mm, 5.0 μm particle size, Merck KGaA, Darmstadt, Germany), with constant flow rate of 1 mL min-1 of 100% solvent A (acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... To perform this, tissues were fixed over a filter paper imbibed with 30% sucrose (Winkler, Chile) in PBS–4% paraformaldehyde (Merck, USA) for at least 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... Individual carotenoids were quantified in a Shimadzu HPLC (LC-10AT) with a diode array detector using a RP-18 Lichrocart 125-4 reverse phase column (Merck®) and a mobile phase composed of acetonitrile:methanol:isopropanol (85:10:5 v/v) ...
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bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Cancer Biology 2021Quote: ... and EV-containing fractions were pooled and concentrated with a 10 kDa molecular weight cut-off Amicon Ultra-4 centrifugal filter unit (Merck Millipore).
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Cell Biology 2022Quote: KELLY cells were grown on 20 x 14 cm dishes and treated for 14-16 hours with 100 µM of 4-Thiouridine (Merck, T4509). Cells (1.6 × 108 ...
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bioRxiv - Neuroscience 2022Quote: ... was either boiled with 4x SDS sample buffer (4% SDS, 40% glycerol, 20% ß-Mercaptoethanol in 250 mM Tris-HCl, Merck) at 95°C ...
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bioRxiv - Biophysics 2022Quote: ... for 10 min prior to fixation after washing out puromycin were fixed with 4% PAF for 30 min at 37°C and subjected to immunoblotting using puromycin antibody (Merck, MABE343). For the negative control ...