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Citations for Merck :
201 - 250 of 2819 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were transferred from gel to a PVDF membrane (Merck, cat. no. IPSN07852). Transfer was carried out for 45 minutes at 110V in transfer buffer (cold room) ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Chengdu, China) for 2 h at 150 mA in 10% transfer buffer [3% Tris–HCl (Aladdin Industrial Corporation ...
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bioRxiv - Immunology 2020Quote: ... Protein was transferred to PVDF membranes (Immobilon-P PVDF .45um, Merck Chemicals BV). Membranes were blocked in 5% fat-free milk (Elk ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then electrotransferred onto a polyvinylidene fluoride (PVDF) membrane (0.45 μm) (Merck by ice-cold liquid transfer for 2 h at 70 V ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
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bioRxiv - Molecular Biology 2023Quote: Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore) utilizing a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Amersham, MERCK). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cell Biology 2023Quote: Wet blot transfer was used to transfer proteins to a PVDF membrane (MERCK). Intercept blocking buffer (927-60001 ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and the RDF plasmid encoding the RD114 envelope) using the GeneJuice transfection reagent (Merck Millipore). Viral supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2.5 × 105 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 µg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Plant Biology 2021Quote: ... α-His6 (anti-polyhistidine tag antibody, Merck), and α-MAR/PAR (anti-pan-ADP-ribose binding reagent ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were blotted onto a 0.45 µm nitrocellulose membrane (Merck, Amersham Protran, Cat#GE10600002) for 2 hr at 4 °C and 30 V ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated on SDS/PAGE gels and transferred onto a PVDF membrane (Merck). After blocking the membrane with 5% fat-free milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Bioengineering 2019Quote: ... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-P; Merck Millipore, MA, USA) using a Trans-Blot® SD semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Biophysics 2023Quote: ... we blotted the proteins at RT on a PVDF membrane (Merck, Cat. No.: IPVH00010). We blocked the membranes for 1 h at RT with sterile filtered 5 % bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed protein solution was filtered through a 0.22 μm membrane (Merck Millipore, SLGP033RB) and injected onto an ion-exchange Q column (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore, IPVH00010). The membranes were blocked ...
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bioRxiv - Pathology 2024Quote: ... the resolved protein samples were transferred onto a PVDF membrane (Merck Millipore Ltd., USA) through a Bio-Rad electrophoretic blotting apparatus ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... E-cadherin (1:300, Merck, #U3254), α-actinin (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... E-Cadherin (1:600, Merck U3254), Glucagon (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...