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Citations for Merck :
351 - 400 of 2819 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Bioengineering 2022Quote: ... coli Rosetta2DE3pLysS (Novagen, Merck, KGaA, Darmstadt, Germany). A single colony derived from the transformation was used to set an overnight culture in a 3mL 2xYT medium at 37 °C with 250 rpm of shacking ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21 (DE3) cells (Merck, Darmstadt, Germany) (from now on denoted as protocol 1 ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) cells (Merck, Darmstadt, Germany) for 2 h after induction with 1 mM IPTG at 37 °C and disrupted under non-denaturing conditions to separate inclusion bodies from lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 (DE3) (Merck KGaA, Darmstadt, Germany) was used for protein expression.
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bioRxiv - Biophysics 2021Quote: ... coli Rosetta (DE3) pLysS cells (Merck 70954) grown in Miller’s LB medium (Formedium LMM105) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3)T1R (Merck Life Science). Cells were co-transformed with the pRARE2 plasmid (Protein Science Facility ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) (Merck, Darmstadt, Germany) was used for the expression of recombinant proteins ...
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bioRxiv - Plant Biology 2024Quote: ... coli strain Rossetta2(DE3)pLysS (Novagen/Merck).
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta (BL21 DE3) competent cells (Merck) by heat-shock transformation or by electroporation ...
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bioRxiv - Immunology 2023Quote: ... coli serotype 0111:B4 (Sigma-Aldrich/Merck) was used as a control of macrophage stimulation ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) (SingleTM competent cells Merck) of a pb6-pLIM13 plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... coli Rosetta2 (DE3) (Merck Millipore, Darmstadt, Germany) was transformed with each expression vector and cultured in lysogeny broth medium containing 100 μg/mL ampicillin and 17 μg/mL chloramphenicol at 37 °C until the OD600 (optical density at 600 nm ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) (Merck Millipore, Massachusetts, USA) transformed with the corresponding expression plasmid ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse anti-Myc tag antibody was purchased from Merck Millipore (Bangalore ...
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bioRxiv - Microbiology 2021Quote: ... pET28a-c (cloning with NH2-terminal 6His tag, Merck), and pMal-c5x (cloning with the NH2-terminal maltose-binding protein (MBP ...
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bioRxiv - Plant Biology 2020Quote: ... α-T7 Tag HRP conjugate 1:10.000 (Merck, Darmstadt, Germany), α-mouse HRP-conjugated 1:10.000 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... the anti-S tag antibody (Merck, Cat# 71549-3) was added directly to the medium of each well resulting in a final dilution of 1:500 ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... were separated by SDS-PAGE and protein was transferred to MeOH-activated Immobilon®-P membrane (Merck Millipore) using Whatman paper and a transfer buffer of 48 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... Protein wet transfer was performed overnight at 4°C using an Immobilon®-FL PVDF membrane (Merck™). Next day ...
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bioRxiv - Developmental Biology 2021Quote: ... The eluted protein was concentrated using ultrafiltration units with 10 kDa regenerated cellulose membrane (Amicon Ultra, Merck Millipore) and subsequently loaded on an equilibrated (50 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting and detection were performed on the PVDF membrane with SNAP i.d.® 2.0 Protein Detection System (Merck) by using anti-myc (A00704 ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Microbiology 2020Quote: ... To investigate the inhibitory activity of E-64 (Merck) and Z-VAD-fmk (Invivogen ...
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bioRxiv - Microbiology 2019Quote: ... we provided the information of praziquantel (E. Merck KG) written in Chichewa to all villagers ...
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bioRxiv - Cell Biology 2023Quote: ... Ultrathin sections were stained with uranyl acetate (E. Merck) and lead citrate (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). PrP proteins were purified by high-performance liquid chromatography on a C4 reverse-phase column (Shimadzu ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(DE3)pLysS (Novagen, now Merck) and expression plasmid carrying AHK5HK-RD gene into ER2566 (NEB ...
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bioRxiv - Biophysics 2020Quote: Escherichia coli BL21 Rosetta™(DE3) (Merck/Novagen) cells harbouring the co-expression vectors were grown at 37 °C in LB medium (Lysogeny-Broth ...
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bioRxiv - Bioengineering 2020Quote: ... coli BL21(DE3) (Novagen, Merck KGaA, Darmstadt, Germany), B ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). SOD1 protein was purified to homogeneity by Q-Sepharose chromatography as describe by Chattopadhyay et al.26 and Xu et al.6 ...
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bioRxiv - Microbiology 2022Quote: ... Lipopolysaccharide (LPS) from Escherichia coli O111:B4 (Merck) was diluted in saline and administered intraperitoneally to S1 mice at 5 mg/kg ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany) and purified by high-performance liquid chromatography on a C4 reverse-phase column (Shimadzu ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli were routinely cultured in LB (Merck) or on L-agar (1.5% [w/v] agar added ...
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bioRxiv - Cancer Biology 2023Quote: ... coli tRNA (Sigma Aldrich, Merck cat. no. 10109541001)/0.02% w/v bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). TDP-43 protein was purified to homogeneity by nickel affinity chromatography as described by Vega et al ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-T7-tag (Merck 69522; RRID:AB_11211744; WB 1:10000) and mouse anti-PSD-95 (Thermo Fisher Scientific MA1-046 ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity tags were cleaved by incubation with either thrombin (Merck) or papain (Thermo Scientific) ...