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Citations for Merck :
51 - 100 of 2819 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked using 2% milk (Merck) with 0.05% of normal goat serum (NGS ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: 2mL SARS-CoV-2 P4 supernatant containing 2 x 107 pfu/mL was purified using an Amicon Ultra column (MWCO 100kDa; Merck) by centrifugation and washing in PBS at 2,000 x g ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Merck) and blocked with 5% (wt/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 3 hours at 60 V with Tris/Boric buffer at 50 mM/50 mM ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred onto PVDF membranes (Merck Millipore) by wet electroblotting for 1 h at 100 V using the Mini-PROTEAN Tetra system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to PVDF membranes (Merck).
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 30 minutes at 100 V with Tris/Boric buffer at 50 mM/50 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were blotted to a PVDF membrane (Merck) for 1-2 h at 4°C with 300 mA constant current using the Bio-Rad immunoblot apparatus ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred onto Immobilon transfer membrane (Merck) using a BioRad Trans-Blot turbo transfer system ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... protein gels were blotted onto PVDF membranes (Merck). Upon blocking with skim milk ...
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 3 h at 60 V or for 30 minutes at 100 V in blotting buffer (48 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (MERCK-Millipore) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto PVDF membranes (Merck Millipore). The membranes were blocked with skim milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was transferred onto a PVDF membrane (Merck) at 60 V for 90 min in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) was transformed with pET28a-His6-CrTRXz and grown in 1 L of 2YT medium supplemented with kanamycin (50 µg.mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ PVDF membrane, Merck) by semi-dry turbo Western blot procedure (Trans-Blot Turbo Transfer System ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were first incubated with anti-T7-tag conjugated with alkaline phosphatase (1:10000, Merck, #6999) for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... the cDNA of the fusion protein was cloned in pET21a (Merck KGaA, Darmstadt, Germany). For preparative overexpression the E ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a PVDF membrane (Merck), which was blocked with 5% dry milk ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Merck). The membranes were blocked with Blocking One for 30 min at room temperature and then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were blocked in 2% skim milk powder (Merck) and 0.5% FCS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Biochemistry 2021Quote: Transformation of Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) were made with a pET3d-His6-CrFBA3 plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were transferred onto Immobilon-P PVDF membranes (Merck IPVH00010) using a wet-transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merck) that were activated in methanol (Carl Roth ...