Labshake search
Citations for Merck :
1951 - 2000 of 2010 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: Medium conditioned by MDA-MB-468 cells was loaded into size exclusion columns (Amicon® Ultra-15 Ultracel-10 Centrifugal Filter Unit, Merck, #UFC901024) centrifuged at 4,000g for 30 min and the fractions stored at -20⁰C until further analysis ...
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bioRxiv - Microbiology 2023Quote: ... and untagged 1H6 (mouse monoclonal IgG2bκ) in PBS with 0.05 % sodium azide and 50 % glycerol were purchased from Merck (stored at -20°C with light protection). We used sterile-filtered 3 % bovine serum albumin (BSA heat shock fraction ...
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bioRxiv - Microbiology 2020Quote: ... Glycolipid extracts of up to 15 μl were applied evenly onto half TLC silica plates (20 x 10 cm, Merck KGaA, Darmstadt, Germany). After drying ...
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Biophysics 2019Quote: ... ES products from all three days were thawed at 4 °C and pooled to be concentrated 720 times with Amicon® Ultra-15 Centrifugal Filter Unit 10 kDa cut-off (Merck, cat#UFC901024). The concentrate was used for EV separation.
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bioRxiv - Bioengineering 2019Quote: ... was diluted with ultrapure water (30 mL LUDOX® TM50 to 400 mL water) followed by addition of ammonia (10 mL) (NH3, 28-30%, Merck, Darmstadt, Germany) and (3-aminopropyl)trimethoxysilane (24 mL ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5E05 cells were seeded onto a T25 Matrigel coated flask in StemFlex medium supplemented with 10 µM rock inhibitor (Merck Millipore, Y-27632). Medium was refreshed the following day without rock inhibitor and lived cells were sent over to Cell Line Genetics when 20-30% confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Molecular Biology 2019Quote: ... was thawed at room temperature and vortexed extensively before being concentrated to 800 µl using a 10 kDa centrifugal filter device (Centricon Plus-70, Merck Millipore, Massachusetts, USA). The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter ...
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bioRxiv - Physiology 2019Quote: ... in 35 mm Petri dishes were incubated for 10 min in steady state E2 medium (controls) or E2 + 50 or 250 mM NaCl (Merck, #106404, Darmstadt, Germany) at 28°C under white light illumination ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Developmental Biology 2022Quote: ... The dehydrated samples were then incubated in a 1:1 mixture of propylene oxide and absolute ethanol for five minutes at RT followed by a two times 10 minutes incubation in pure propylene oxide (Sigma-Aldrich, Merck, Darmstadt, Germany), and a 30 minutes incubation in a 1:1 mixture of propylene oxide and Spurr resin (Low Viscosity Spurr Kit ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining peptides were loaded onto primed custom-made HILIC SPE micro-columns packed with ZIC-HILIC resin (10 μm particle size, 200 Å pore size, kindly provided by Sequant/Merck, Umea, Sweden) onto supporting C8 disks (Empore ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The protein preparation was then concentrated to 300 – 500 μL volume using Amicon Ultra centrifugal filter units with 10 kD cut-off (Merck KGaA, Darmstadt, Germany) and injected into Superdex 75 10/300 GL size exclusion column attached to ÄKTA micro chromatography system (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were dissolved in 50 μL of Milli-Q water and filtered (0.20 μm pore size, 10 mm diameter, Merck Millipore, Burlington, MA, US)71.
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bioRxiv - Cell Biology 2023Quote: ... Peak fractions were pooled and concentrated using an Amicon Ultra-0.5 centrifugal filter device with a molecular weight cut-off of 30 or 10 kDa (Merck, cat. # UFC503024 or UFC501008). D-desthiobiotin in the peak fraction was diluted more than 100-fold by a repeating dilution with EB and the centrifugal concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... The resulting Fabs were buffer exchanged into PBS and then concentrated down using Amicon® 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9010). Selected fractions resulting from the size-exclusion chromatography were pooled together and concentrated again for later use.
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bioRxiv - Neuroscience 2023Quote: ... dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 µg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]).
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... TSPO-/- MPAs were grown in T25 flasks until confluent before being stored in liquid nitrogen (10% v/v dimethyl sulfoxide (DMSO; catalogue # D2650, Merck Life Sciences (UK)) until TSPO genotypes had been confirmed ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Biochemistry 2023Quote: Purified NanoLuc was concentrated to a final concentration of ∼10 mg/mL using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). Concentrated NanoLuc was mixed with a 4 molar excess of FMZ non-oxidizable analogue azaFMZ (stock solution of azaFMZ was 10 mM in isopropanol) ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times with 200 µl/well of PBS/Tween and incubated for 10 min at room temperature with 50 µl/well of 3,3‘,5‘,5‘-tetramethylbenzidine (TMB)/H2O2 peroxidase substrate (Merck KGaA, cat. no. T4444). The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate was concentrated down to 1.5 mL using an Amicon Ultra Centrifuge Filter unit (30 kDa cutoff for GST-UtrCH-sfGFP and 10 kDa cutoff for GST-LifeAct-sfGFP; Merck, UFC903024 and UFC901024, respectively). The GST tag was removed by digestion with 8 units/mL PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... [11] were maintained in Dulbecco’s modified Eagle medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Merck, Kenilworth, New Jersey, USA) and 1 mg/ml of G418 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were buffer exchanged into PBS and then concentrated down using Amicon® 100 kDa or 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9100 & UFC9010), respectively ...