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Citations for Merck :
2001 - 2050 of 2192 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Neuroscience 2024Quote: ... Forebrain slices 250 µm thick were transferred in Millicell chambers and cultured for 4 hours in F12/DMEM medium supplemented with CFS 10% in a CO2 incubator (Merck Millipore) prior to pharmacological treatment and recording ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Cell Biology 2023Quote: ... except that SDS-PAGE was carried out using Millipore mPAGE® 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... were homogenized (60 Hz, 90 s) and extracted by ethanol three times (95%, 3 × 300 mL, vortex 10 min each, Merck, Germany). The three batches of extracts were pooled together and centrifuged for 20 min at 10000g and 4°C (Bruker ...
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bioRxiv - Biophysics 2024Quote: ... nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra, 10 K MWCO, Merck Millipore).
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated using an Amicon Ultra 10 kDa molecular weight cutoff centrifugal filter unit (Merck Millipore). The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were subsequently filtered through 10 kDa followed by 3 kDa Amicon Ultra-0.5 centrifugal filters (Merck KGaA, Darmstadt, Germany) to generate sub-cellular fractions with molecular size of larger than 10 kDa ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatants were exchanged three times to the same buffer as above (Amicon Ultra 0.5 mL, 10 kDa cut off (Merck, Darmstadt, Germany) (10,000 g for 20 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Biophysics 2023Quote: Rec samples were transferred to a labeling buffer (50 mM HEPES, pH=7.0, 5 mM EGTA) using 10 kDa MWCO Amicon Ultra centrifugal filter unit (Merck Millipore, USA), and concentrated to 0.5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Systems Biology 2023Quote: ... Media was designed based on the Delft composition with a glucose concentration of 10 g/L and the addition of 1 mL/L of Antifoam 204 (Merck, Germany).
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer exchanged with SnakeSkin™ dialysis tubing (10 MWCO, Pierce, 68100) followed by additional dialysis and concentration with Amicon Ultra-15 50 kDa centrifugal units (Merck, UFC905008). Purified protein was conjugated to AlexaFluor647 (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Washed DPI-treated VBNC Mtb cells were resuspended with fresh Dubos broth and the suspension was diluted to 1:10 with Dubos broth with 0.1% (w/v) BSA containing adenylyl cyclase inhibitor SQ22536 (Merck, Darmstadt, Germany) or protein kinase inhibitor H89 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Genomics 2022Quote: ... During sampling we set a flow rate of 200 l/minute for a total of 60 minutes with 10 ml 0.05 % (v/v) Triton X-100 (T8787-50ML, Merck Sigma Aldrich) as collection buffer prepared with UltraPure DNase/RNase free ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto Ni-NTA Superflow resin and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto the Ni-NTA Superflow cartridges and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S75 (26/600 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
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bioRxiv - Plant Biology 2023Quote: ... a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa, Merck).
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Sections were rinsed in 0.1 M PBS three times each for 10 min and then incubated with: i)1:1000 anti-NeuN (A-60; Merck Millipore), 1:300 anti-ΠIII-Tubulin (Tuj1 ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed them six times for 10 min with 1 % Triton (Triton X-100, X100-100ML, Sigma Aldrich, Merck, Germany) in PBS (thereafter named 1 % PBST ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... female mice aged 6-8 weeks were stimulated to ovulate by 10 IU intraperitoneal injection of Foligon/PMSG (MSD Animal Health, Spain) followed by intraperitoneal administration of 10 IU of Ovitrelle 250 µg/0.5 mL (Merck Serono, Germany) 48 h later ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer56 for storage at −80℃.
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were resolved on a 10% SDS-PAGE gel and Western blotting was performed using an anti-myc antibody (Merck, UK). Co-immunoprecipitation of Ssk2-HA was detected using an anti-HA antibody (Merck ...
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bioRxiv - Biophysics 2024Quote: ... The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250, Merck) to confirm its purity ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in a 10-cm-diameter tissue culture dish (IWAKI) with BIS medium under an anaerobic condition using Anaerocult® A (Merck). Trophozoites were detached from the surface of the dishes by adding cold PBS into the dishes and incubating them at 4℃ for 10 minutes ...
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The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomesbioRxiv - Neuroscience 2024Quote: ... with 7.5-10 μg of HCT in PBS in a volume of ∼3.5 µl using a 701 N Hamilton® syringe (Merck, 20,779) for TA ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
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bioRxiv - Microbiology 2024Quote: Asexual spores of the strain HEK1 were collected from a fully colonized plate (10 cm diameter) with 15 mL of Tween® 80 (0.016%) (Merck, DE). The resulting suspension was centrifugated (Sigma 2-16PK ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Biochemistry 2024Quote: ... equipped with a capillary column (SUPELCOWAX™ 10; 30 m x 0.32 mm i.d. x 0.25 μm, Merck KGaA, Darmstadt, Germany). The injection port and ion source temperatures were 250 °C and 200 °C ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 hour with HRP-conjugated secondary antibodies (1:5000, anti-mouse, Sigma-Aldrich Catalogue No A9044; Flag, or 1:10000, anti-rabbit, Merck, Catalogue No GENA934; LpoA) in TBS-TM ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...