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Citations for Merck :
1551 - 1600 of 2010 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Hela Kyoto and FERRY subunit knock-out cells were cultured in DMEM media supplemented with 10% FBS Superior (Merck) and 50 μg/ml streptomycin (P/S ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... in Ca2+ and Mg2+ free medium (10% Krebs Solution (133 mM NaCl, 177 mM KCL, 1.75 mM NaH2PO4, all Merck), 1% MEM Non-Essential Amino Acids Solution (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck). The cells were incubated with the medium for 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
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bioRxiv - Bioengineering 2023Quote: ... the medium reservoirs were aspirated and 50 μL EGM-MV2 containing 100 ng/mL 10 kDa FITC-dextran (Merck) added to a single reservoir on each side of the device ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed trice for 10 min with 0.05% Tergitol in TBS (Tris-Buffered Saline) and blocked for 1 hour with 2% BSA (Merck) in TBS ...
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bioRxiv - Genomics 2023Quote: ... DBCO-PEG4-conjugated antibodies were concentrated using an Amicon Ultra-0.5 NMWL 10-kDa centrifugal filter (Merck Milipore, UFC501024) and measured on a NanoDrop™ 2000 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Immunology 2023Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
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bioRxiv - Biochemistry 2023Quote: ... The chromatographic separation of anionic phospholipids was performed on a reverse phase C18 column (SUPELCOSIL™ ABZ PLUS; 10 cm x 2.1 mm, 3µm, Merck) using methanol:water (3:2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Microbiology 2023Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Plant Biology 2023Quote: ... The resin was pelleted as above and washed twice with 0.8 mL plant extraction buffer (see plant protein extraction section above) supplemented with 10 μM MG132 (Merck). Cold plant extract was either added directly to the resin (Supplementary Dataset S1 ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was filtered through Amicon® Ultra – 0.5 mL 10-kDa cutoff centrifugal filters (Merck KgaA; Darmstadt, Germany). To test the enzymatic efficiency of DisA before synthesizing [32P]c-di-AMP ...
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bioRxiv - Cell Biology 2023Quote: ... and then embedded in BMM resin (butyl methacrylate, methyl methacrylate, 0.5% benzoyl ethyl ether with 10 mM DTT (Merck)) at -20°C under UV light for polymerization ...
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bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using a centrifugal filter unit (Amicon Ultra, 10 or 30 kDa cut-off, Merck), and the solvent was exchanged with 50 mM potassium phosphate buffer (pH 7.0 ...
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bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... then layered over 5ml of 70% Percoll in a 15ml tube (Percoll Merck P4937, 10% 10x PBS, 20% RPMI). The tube was then centrifuged at 1450rcf for 11mins with the break set to 1 and accelerator to 3 ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... protein thioesterase inhibitors (10 µM palmostatin and 0.2 mM HDSF (1-hexadecanesulfonyl fluoride)) and 250 U/ml Benzonase Nuclease (Merck). After 30 min (4°C) ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitor was added to sample and sample was concentrated down to 150 µL using Amicon Ultra-0.5 10 kDa Centrifugal Filter Units (Merck). On the other hand ...
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bioRxiv - Biochemistry 2024Quote: ... Purified MPP8 CTD was concentrated to 4.5 mg/ml using a 10-kDa cutoff centrifugal filtration device (Merck Millipore) and mixed with an equal volume (up to 0.5 µl ...
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bioRxiv - Biochemistry 2024Quote: The lysate was cleared by centrifugation at 12,000g for 30min at 10°C and the supernatant was applied onto a Talon Superflow beads batch (Merck). The immobilized proteins were washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... The animals used in our experiments were genotyped for expression of the transgene by quantitative PCR (qPCR) using the KAPA Mouse genotyping kit (Merck, Cat# MGKITKB-KK7301), with primer sequence (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... SPN infected A549s were fixed with chilled methanol followed by further processing with Duolink® In Situ Orange Starter Kit Mouse/Rabbit (MERCK, DUO92102-IKT) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...