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Citations for Merck :
151 - 200 of 660 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were loaded on SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Merck Millipore, MA, USA). Membranes were incubated in blocking solution (TBS ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... resolved by SDS-polyacrylamide gel electrophoresis and subsequently transferred to nitrocellulose membranes (Amersham Protran Western Blotting Membranes; Merck). The filters were blocked with TBS containing 0.05% Tween and 5% non-fat dry milk (Merck) ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Bioengineering 2019Quote: ... 40 µg of total protein were run on a 12% SDS-PAGE and transferred onto a 0.45 µm Immobilon®-P Transfer Membrane (Merck Millipore, Cat. NO#IPVH00010). The membrane was hybridized with a custom antibody at a 1 µg/mL dilution (GenScript ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...
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bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
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bioRxiv - Cancer Biology 2019Quote: ... Subsequently 20 μg of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membranes (Immobilon-P PVDF, EMD, Merck,). Membranes were blocked with 5% fat-free milk in TBST (0.01% Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... and lysed in SDS sample buffer containing 100 mM DTT before protein separation on precast Bis-Tris polyacrylamide gels (MPAGE; Merck) and transferred to nitrocellulose membranes by electroblotting ...
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bioRxiv - Biochemistry 2023Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 10% SDS (Merck), 35% glycerol (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were subsequently transferred (Transfer Buffer 25mM Tris, 190mM Glycine, 20% Methanol, 0.1% SDS) from the gel onto a PVDF membrane (Merck, Cat#IPVH07850) using a wet transfer system ...
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bioRxiv - Cell Biology 2023Quote: ... except that SDS-PAGE was carried out using Millipore mPAGE® 10% Bis-Tris Precast Gels (MP10W10/ MP10W12/ MP10W15) with MES SDS running buffer (MPMES) from Merck, before protein transfer to PVDF membranes.
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bioRxiv - Cell Biology 2020Quote: ... were separated with a 10% SDS-PAGE resolving gel using the Laemmeli discontinuous buffer system and then transferred to a PVDF transfer membrane (Immobilon-FL, Merck KGaA, Darmstadt, Germany) using a Biorad wet transfer apparatus ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of protein per sample were size-separated by electrophoresis on SDS-polyacrylamide gels and electroblotted onto Immobilon®-P polyvinylidene fluoride (PVDF) transfer membranes 0.45 μm pore size (Merck Millipore Ltd., Cork, IRL). After 1 h of blocking at room temperature with 5% skim milk in PBS 0.1% Tween ...
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bioRxiv - Systems Biology 2024Quote: Cells were analysed for protein expression by SDS polyacrylamide gel electrophoresis and Western blot analysis using the following antibodies: mouse monoclonal anti α-tubulin (#05-829, Merck Millipore, Burlington, US), rabbit α-p-AKTSer473 (#9271) ...
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bioRxiv - Physiology 2021Quote: ... permeabilized with 0.1 % SDS (Merck) in PBS for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% SDS (Merck,75746-1KG), 10 mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... single-use foam swab (Merck – product was discontinued) by rubbing one side of the swab back and forth approximately ten times across a ~10×10 cm area of the tank wall about 6 cm below surface water level and repeating the procedure on the same area with the other side of the swab ...
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bioRxiv - Cell Biology 2021Quote: ... 20% sodium dodecyl sulphate (SDS) (Merck 428018), and 20 μL of 20 mg/ml proteinase (Ambion RNA by Life Technologies AM2548)] ...
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bioRxiv - Cancer Biology 2021Quote: ... single cells were fixed in (PFA) (#1004965000, Merck Millipore) at RT for 10 min and permeabilized overnight on ice with 70% EtOH ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% SDS) added with 1X PIC (Merck, #539131) and Benzonase (NovoProtein ...
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bioRxiv - Cell Biology 2022Quote: ... pre-coated silica gel plates (Merck TLC silica gel 60 F254). Spots were detected by a UV lamp under 254 nm or 365 nm wavelength ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by filtering through a single layer of Miracloth (Merck). After that ...
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bioRxiv - Microbiology 2022Quote: ... Single colonies were inoculated into Mueller-Hinton broth (Merck, UK) and incubated overnight at 37°C with agitation at 200r.p.m.
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bioRxiv - Microbiology 2023Quote: ... Single colonies were inoculated into Mueller-Hinton broth (MHB) (Merck) and incubated overnight at 37°C with agitation at 200 r.p.m.
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bioRxiv - Neuroscience 2022Quote: ... was either boiled with 4x SDS sample buffer (4% SDS, 40% glycerol, 20% ß-Mercaptoethanol in 250 mM Tris-HCl, Merck) at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel (E, Merck) was used for flash chromatography ...
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bioRxiv - Developmental Biology 2021Quote: ... 1X complete protease inhibitor (Roche)] including 1% SDS and 1 U/µL benzonase (Merck). After 10 minutes of incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 % sodium dodecyl sulfate (SDS) (Merck, cat. no. L6026)) supplemented with 1x cOmplete™ Protease Inhibitor Cocktail (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% SDS) with 1 × protease inhibitor cocktail (Merck, 04693132001). The cell lysates were centrifuged at 20,000 g for 15 min at 4°C and resuspended in TN buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... in mPAGE MOPS SDS Running Buffer (Merck Millipore, MPM0PS) and transferred onto nitrocellulose membranes (Invitrogen iBlot 2 Transfer Stacks ...
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bioRxiv - Biochemistry 2022Quote: ... Single colony was inoculated into 1 ml of LB media (Merck) and incubated overnight ...
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bioRxiv - Microbiology 2023Quote: ... a single colony was isolated using cloning cylinders (Merck, Darmstadt, Germany) and propagated ...
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bioRxiv - Microbiology 2020Quote: ... the fractions were diluted in 4x SDS sample buffer (Merck) and heated at 95 °C for 5 min prior to fractionation by SDS-PAGE ...
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bioRxiv - Systems Biology 2022Quote: ... 1% sodium dodecyl sulphate (SDS) (Merck Sigma, Cat. No. L4522) or 8 M Urea (Merck Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Sodium dodecyl sulphate (SDS) was purchased from Merck (Darmstadt, Germany). Trypsin and Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2020Quote: ... TLC silica gel plate (MERCK), ethanol p.a (Merck) ...
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bioRxiv - Microbiology 2022Quote: ... single colonies were resuspended in 10 ml TOS-Propionate broth (Merck, Germany), which was made according to manufacturer’s instructions while agar was removed by filtration over a 520B qualitative filter paper (Whatman ...
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bioRxiv - Biochemistry 2022Quote: ... with single element standards (Chloride is MERCK and others are SPEX CertiPrep). The samples were collected and made up 5mL with 2 v/v % HNO3 ...