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Citations for Merck :
1 - 50 of 660 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... or 14% SDS-PAGE gel and transferred onto PVDF membrane (Merck, ISEQ85R) using a semi-dry transfer unit (Bio-Rad Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... WB was performed with homemade SDS-PAGE gels and nitrocellulose membranes (Merck). The following antibodies were used ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were then transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore) and incubated with primary antibodies diluted in TBS-T (20 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated on SDS/PAGE gels and transferred onto a PVDF membrane (Merck). After blocking the membrane with 5% fat-free milk in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run using 10% SDS-PAGE gel and transferred to nitrocellulose membrane (Merck). Membranes were blocked with 5% fat-free milk in TBS-Tween20 0.1% and incubated overnight with primary antibodies listed in the Supplementary Table 7.
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bioRxiv - Developmental Biology 2019Quote: ... reduced samples were resolved on 10% SDS-PAGE gels and blotted onto PVDF membranes (Merck). Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck ...
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bioRxiv - Developmental Biology 2020Quote: ... reduced samples were resolved on 10% SDS-PAGE gels and blotted onto PVDF membranes (Merck). Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... A loading dye (4X SDS-page, Merck) was added to the samples to a final concentration of 1X and reactions were denaturated at 98°C for 5 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an AmershamTM HybondTM 0.45µm polyvinylidene difluoride (PVDF) membrane (Merck). CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
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bioRxiv - Cell Biology 2024Quote: ... separated by SDS-PAGE (10% acrylamide, Merck Millipore), transferred to nitrocellulose membrane (0.2 µm ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Cell Biology 2020Quote: ... Following SDS-PAGE analysis the gels were fixed in fixation buffer for 30 min and incubated in DMSO (Merck) for 2 × 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Molecular Biology 2019Quote: ... For gel assay reaction mixtures were loaded onto 12% SDS PAGE and then transferred onto PVDF membrane (Merck Millipore) and immunoblotted with antibodies as mentioned ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed on pre-cast Tris-Acetate gels followed by transfer to PVDF transfer membrane (Merck Millipore). Proteins were detected using primary antibodies as described followed by incubation with secondary antibodies coupled with HRP (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a 4–15% gel by SDS-PAGE and transferred to PVDF-FL membranes (IPFL00010, Merck). Membranes were blocked with 5% non-fat milk in PBS and incubated with primary antibodies diluted in 5% non-fat milk in PBS+0.1%Tween-20 (PBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... Equal concentrations of the samples were resolved on SDS-PAGE gel and electroblotted onto PVDF membranes (Merck Millipore, USA) (Srivastava and Panda ...
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bioRxiv - Plant Biology 2020Quote: Total protein extracts were separated by SDS PAGE on an acrylamide gel before being electrotransferred on a PVDF membrane (0.45 μM, Merck millipore). After saturation in a (1X TBS −0.1% tween - 5% fat-free dry milk ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Cell Biology 2021Quote: The samples were loaded onto a 5–20% gradient SDS-PAGE gel (Wako, Osaka, Japan) and transferred to an Immobilon-P Transfer Membrane (Merck). Antibodies were diluted with Signal Enhancer HIKARI for Western Blotting and ELISA (Nacalai Tesque) ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μg of protein were resolved by 8% dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a 0.45 μm polyvinylidene difluoride membrane (PVDF; Immobilon, Merck Millipore). Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Cell Biology 2023Quote: Whole liver lysates and lipid droplet-enriched fraction proteins were loaded on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Merck). Membranes were incubated at room temperature for 1 h with blocking buffer (TBS ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were loaded on 8% gels and separated by SDS-PAGE followed by the transfer done on to Immobilon-P® (Merck) PVDF membranes at 106 V for 75 minutes.
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bioRxiv - Molecular Biology 2022Quote: ... protein lysate (30 μg) was loaded onto and run through a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck, IPVH00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Laemmli running buffer and transferred to PVDF membranes (Merck-Millipore) in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon®-P PVDF membrane (Merck), followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membrane (PVDF Transfer Membrane, Merck-Millipore). The primary antibodies used for the detection of proteins are Anti-CRY1 (Bethyl) ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...