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Citations for Merck :
301 - 350 of 660 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Neuroscience 2021Quote: Type 1 diabetes was induced with a single intraperitoneal (i.p.) injection of 150 mg/kg streptozotocin (STZ; Merck, France) freshly dissolved in 0,9 % NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies from those plates were inoculated in 5 mL of tryptic soy broth (TSB; Merck KGaA, Darmstadt, Germany) and incubated for 16 - 18 h at 37°C with shaking at 200 rpm ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck] ...
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bioRxiv - Immunology 2024Quote: ... splenic single-cell suspensions were depleted of red blood cells (RBCs) by using RBC lysis buffer [0.15 M NH4Cl (Merck), 1 mM KHCO3 (Merck) ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions were depleted of red blood cells (RBCs) by using RBC lysis buffer [0.15 M NH4Cl (Merck), 1 mM KHCO3 (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... and SDS-PAGE and immunoblots were performed by standard methods using a mouse monoclonal anti-MYC antibody (clone 4A6, 05-724, Merck).
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bioRxiv - Biophysics 2019Quote: ... Peak fractions monitored by SDS-PAGE were collected and exchanged with the storage buffer using AmiconUltra-15 10k and AmiconUltra-0.5 10k filters (Merck Millipore). Samples were stored at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by SDS-PAGE and those containing the target protein were pooled and concentrated with Amicon Ultra Centrifugal Filter Units 10K MWCO (Merck). GbpA was further purified by SEC using a Superdex 200 Increase 30/100 GL column (Cytiva ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.25% sodium deoxycholate and 0.1% SDS (AppliChem) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II and IV (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 heads per sample were homogenised in 100 μL 2% SDS buffer (Cat No. 428018, Merck) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Flash chromatography was performed on a Varian 971-FP flash purification system using silica gel cartridges (Varian, particle size 50 μm) or on glass columns using silica gel type 60 (Merck, particle size 230 μm, 400 mesh). All compounds were obtained as oils ...
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bioRxiv - Cell Biology 2020Quote: ... Gel slices were washed then dehydrated with 100% acetonitrile (Merck Millipore), incubated with 10mM DTT (Dithiothreitol ...
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bioRxiv - Cell Biology 2020Quote: ... gels were transferred onto PVDF membranes (0.45 μM Immobilon-P, Merck) using the Owl HEP-1 Semidry Electroblotting system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel image was taken by GeNei imaging system (Merck, USA).
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bioRxiv - Microbiology 2019Quote: ... TLC plates (3 × 10 cm) (TLC Silica Gel 60 F254, Merck) were spotted by simply touching the end of a capillary tube containing fungal crude extract to the coated side of the TLC plates ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were transferred onto activated PVDF membranes (Immobilon Psq, 0.2µm, Merck) prior to blocking and incubation with the indicated primary antibodies.
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bioRxiv - Biochemistry 2020Quote: ... TLC was done on glass-backed Silica Gel 60 plates (Merck) with chloroform/methanol/water (10/10/3 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Column chromatography was performed on silica gel (Merck, 60 to 120mesh). Infrared spectra (IR ...
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bioRxiv - Microbiology 2020Quote: ... Gels were blotted on PVDF membrane (Merck, PSQ membrane, 0.2 μm) for 15 - 20 min at 12 V and subsequently blocked in nonfat dried milk at 4°C over night ...
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bioRxiv - Systems Biology 2020Quote: ... Gels were transferred to ethanol activated PVDF membranes (Merck IPVH00010-IN) at 90V for 2h ...
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bioRxiv - Cell Biology 2021Quote: ... Thin layer chromatography (TLC) was performed on silica gel plates (Merck) with fluorescent indicator ...
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bioRxiv - Microbiology 2022Quote: ... The sample was spotted onto TLC Silica gel 60 F254 (Merck) and the plate was developed in chloroform:methanol:water (65:25:4 v/v) ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerized gels were rinsed shortly with 1 M NaCl (#7647145, Merck) and afterwards extensively soaked in disruption buffer ...
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bioRxiv - Cell Biology 2022Quote: ... glass-bottomed plates (HPTLC silica gel 60, 10×10 cm, Merck). Pure standards of FA 6:0-NBD (Cayman Chemical ...
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bioRxiv - Microbiology 2022Quote: ... gels were electroblotted on the PVDF membrane (Immobilon-FL, Merck-Millipore). The membrane was blocked using a commercial blocking buffer (BIORAD ...
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bioRxiv - Microbiology 2023Quote: ... TLC plates (Silica gel 60, non-fluorescent, 0.25mm thick, Merck Millipore) were cut to a width that allowed ∼1cm between each lipid sample ...
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bioRxiv - Biochemistry 2023Quote: ... we used anti-FLAG M2 Affinity Gel from Merck (CAT A2220) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were finely dissected into tiny pieces and enzymatically digested into single cells by incubating them in a dissociation solution containing 0.125 mg/ml Collagenase (Merck, C9891), 1 U/ml Dispase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... Lgr5+ cells in the mice were ablated with a single intraperitoneal dose of Diphtheria Toxin (DT) (Cat # 322326-1mg, Merck) in saline (50 µg kg−1).
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Biochemistry 2023Quote: 500 ng – 5 µg of pre-purified rRNA subunits (20 µL of each) were digested to single nucleosides by using 2 U benzonase nuclease (> 90 % purity, Merck) 0.2 U phosphodiesterase I (VWR) ...
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bioRxiv - Cell Biology 2023Quote: ... We designed gRNAs using the Eukaryotic Pathogen CRISPR gRNA Design Tool 63 and ordered forward and reverse sequence as unmodified single-stranded oligonucleotides (Merck). After annealing of the forward and reverse oligonucleotide ...
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bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Both fractions were run on an SDS-PAGE using 12% ClearPageTM gels and ClearPAGETM SDS-R Run buffer (C.B.S. Scientific) followed by staining with Coomassie Brilliant blue R-250 (Merck). Western blot analysis was performed as described elsewhere33.
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... human iPSC cells were detached using Accutase for 5min at 37 °C and followed dissociated into single cells with phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA). Cells were centrifuged at 300 rpm for 5 min and the pellet was resuspended in hESC medium (20% knockout serum replacement ...
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bioRxiv - Genetics 2023Quote: Chemical extractions of single wasps were performed by immersing them in 50 µl HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) in 2 ml glass vials (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) used for DDK immunoprecipitation was washed with the RIP wash buffer and was incubated with Nuclei Lysates at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... kept at room temperature for 3 hours with silica gel (Merck, 101969) and then stored at −80°C for further use.
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bioRxiv - Plant Biology 2020Quote: ... As stationary phase a TLC silica gel 60(Merck KGaA, Darmstadt, Germany) was used in combination with butanol:water:acetic acid (4:1:1 ...
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bioRxiv - Microbiology 2020Quote: ... TLC was performed using silica gel 60 F254 aluminium plates (Merck, Germany). Extracts of camptothecin (10 μl ...
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bioRxiv - Microbiology 2021Quote: ... polyacrylamide gel electrophoresis and general laboratory reagents were from Merck (Moscow, Russia).
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bioRxiv - Microbiology 2021Quote: ... gels were electro blotted on the PVDF membrane (Immobilon-FL, Merck-Millipore). Blocking of membranes was carried out by incubation with either a 5% BSA solution or the proprietary Odyssey Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Genetics 2020Quote: ... Gels were blotted on PVDF membranes (Immobilon-PSQ Membrane, Merck Millipore; ISEQ00010) and membranes were stained with the following primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... The stationary phase (absorbent) used is column graded silica gel 60G (MERCK) while combinations of hexane ...
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bioRxiv - Microbiology 2021Quote: ... gels were incubated in 1 ml of 594 NHS-ester (Merck: 08741) diluted at 10 μg/mL in PBS for 1 hour and 30 minutes at room temperature on a shaker ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... on a 4-20% precast Trupage gel (#PCG2012-10EA; Sigma-Aldrich/Merck). For Western blotting ...