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Citations for Merck :
1901 - 1950 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and glucose (20 mM D-Glucose, Merck), alone or in combination ...
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bioRxiv - Biochemistry 2024Quote: ... 20 g/L D-raffinose (Merck, #R0250), and 25 μg/mL chloramphenicol (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... p-nitrophenyl-β -D-glucoside (pNPG) (Merck) and cellobiose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cetrotide (0.25 mg/d; Merck Serono, Germany) was prescribed ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Bioengineering 2022Quote: ... gels were washed for around 2-4 h with PBS and incubated in 1 ml 1 μM Alexa Fluor 546 NHS ester (NHS-AF546, #A20002, Merck), overnight at room temperature on a slowly rotating shaker ...
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the culture medium was removed and replaced with RPMI medium containing 1 µg/ml D-Actinomycin (Merck, Germany) and different samples of rhTNFα or rhTNFα incubated with TNFα antagonist ...
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bioRxiv - Microbiology 2022Quote: ... and protein expression was induced O/N with 0.5 mM isopropyl 1-thio ß-D-galactopyranoside (IPTG) (Merck Chemicals). The next day ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... AGBs (4 mm) and glass beads (4 mm, Merck, Germany) were inoculated with P ...
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bioRxiv - Biochemistry 2021Quote: ... chloroform containing amylenes as preservative for triglyceride extraction and deuterated chloroform for triglyceride 1H and 13C NMR analysis were all obtained from Merck (Merck Portugal, Algés, Portugal).
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bioRxiv - Neuroscience 2023Quote: ... + 5% HS + 5% donkey serum (DS, Merck - D9663) at RT for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... 4% sucrose (Merck) in PBS for 20 minutes in room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with mEM48 (1:500, Merck Millipore, Cat. # MAB5374) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were applied overnight at 4°C (monoclonal anti–IdU 1:4000; SAB3701448, Merck). Sections were incubated in biotinylated secondary antibodies (Jackson ImmunoResearch Labs ...
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bioRxiv - Biochemistry 2024Quote: ... and blocked overnight at 4°C with 1% bovine serum albumin (BSA, Merck, Cat# 7906) in PBS (200 µl per well) ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Microbiology 2024Quote: ... Low phosphate M9 (lpM9) – 1.28 g/l Na2HPO4 (Merck Ref: 231-448-7), 0.3 g/l KH2PO4 (VWR Ref ...
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bioRxiv - Physiology 2022Quote: ... frozen fresh tissue (∼20-45 mg) was placed into a 2 ml sterile microcentrifuge tube pre-loaded with ∼15-20 glass beads (Merck) while 200 μL of ice-cold methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 ml of pre-warmed 2xSSC with or without RNAseA (50 μg/ml final concentration, Merck) and incubated 1h30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated in standard assay buffer at 37°C and 2 µL aliquots were removed at appropriate time points and spotted on a silica gel 60 F454 plate (Merck). The separation was carried out in butanol:ethanol:milliQ water (5:3:2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse LL/2 (LLC1; ATCC no. CRL-1642; obtained in 2015) was grown in BME with Earle′s salts (Merck) with the same supplementation as mentioned above ...
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bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... a five-day-old larva was embedded at the bottom of the central glass compartment in 2% low-gelling temperature agarose (Merck). The agarose was carefully removed around the eyes and mouth upon hardening and the arena was filled with 3 mL water ...
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bioRxiv - Cell Biology 2020Quote: 5×106 cells were stained with appropriate antibodies for 20min on ice in PBS + 2% FCS and analyzed on an ImageStreamX MKII imaging flow cytometer (Merck). Mfge8-eGFP+ cells were gated using the IDEAS software ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Genomics 2021Quote: ... serial 10-fold dilutions were prepared from the viral stock and used to transduce mESCs in a 6-well plate (Mock plus 10-2 to 10-6) together with 8 ng/µl polybrene (Merck) in duplicates ...
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bioRxiv - Genetics 2021Quote: ... 5 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−2 to 10−6) for transduction with 8 ng/µl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore Darmstadt, Germany), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
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bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
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bioRxiv - Biophysics 2022Quote: ... Buffer was changed to 1Xpbs (Biological Industries, Israel) using multiple washes on Amicon ultra-2 columns (Merck, Burlington, MA #UFC203024).
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bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragmented RNA was subjected to two rounds of m6A immunoprecipitation for 2 hours each using an anti-m6A antibody (ABE572, Merck) previously conjugated to protein-A magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
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bioRxiv - Plant Biology 2022Quote: Fungal elicitor mix was prepared as published previously (24, 38) and separated via enrichment using Discovery DSC-C18 (2 g) columns (Merck) with a H2O/MeOH gradient from 100 % to 0 % H2O in 10 % steps ...
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bioRxiv - Developmental Biology 2024Quote: ... euthanized by tricaine-overdose and fixed with 2.5% glutaraldehyde (Agar Scientific, Stansted, Essex, UK) and 2% paraformaldehyde in 0.1 M Na-Cacodylate buffer (Merck, Darmstadt, Germany) with a pH of 7.44 ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 1 L of freshly 0.1 µm filter-sterilised seawater (n = 2) was filtered onto a 0.2 µm Sterivex (Merck Pty Ltd) filter and snap-frozen in liquid nitrogen ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...