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Citations for Merck :
1801 - 1850 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). The Deep Vent exo- DNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 2 twice with PBS and were permeabilized with PBS–0.06%-Triton X-100 (Merck, USA) for 10 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Prior to sterilization pH of ASW was calibrated at 8.5 ± 0.5 through the addition of 2 M NaOH (Merck) solution ...
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bioRxiv - Molecular Biology 2021Quote: ... thawed pellet was resuspended into 20 ml lysis buffer (0.025 M Tris pH 8; 0.5 M NaCl; 2 mM MgCl2; 100 U/ml Benzonase (Merck); 0.25 mg/ml lysozyme (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with 2 µl of 0.5 M EDTA and separated using thin layer chromatography plates (Merck) with 0.3 M LiCl and 0.3 M formic acid as the mobile phase ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human HepaRG hepatoma cells (1.5-2×105 cells/cm2, Biopredic International, Rennes, France) were cultured in William’s E medium (Merck) supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine and antibiotic/antimycotics and decidualized with 1µM PGE2 or 1µM relaxin-2 (accession # P04090) (both Biotechne Ltd, Abbington, U.K.) in combination with 1µM medroxyprogesterone acetate (MPA) (Merck) for up to 4 days ...
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bioRxiv - Zoology 2024Quote: ... The sample was then placed in 2 ml petroleum ether (boiling range 40-60°C, Merck, Darmstadt, Germany) at room temperature for two days ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Microbiology 2022Quote: ... concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 or 10,000 Da cut-off filter) and stored in aliquots at −80 °C until needed ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Microbiology 2023Quote: ... A fraction of the inoculum was directly transferred into 2 ml tube containing TRI reagent (Sigma-Aldrich, Merck) and silica beads (0.1 mm diameter ...
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bioRxiv - Biophysics 2023Quote: ... 5 and 10 μl of the lipid extract 2 cm from the bottom on aluminum-backed silica gel 60 TLC plates (cat#: 1.05553.0001; Merck) together with DOPG ...
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bioRxiv - Molecular Biology 2022Quote: ... eggs rafts were hatched in trays containing 2 L of Milli-Q water (Synergy® UV, Merck, Germany). Larvae were fed continuously until the pupae stage with TetraMin® baby fish food (Tetra ...
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bioRxiv - Cancer Biology 2024Quote: ... to assess migration the lower faces of the inserts were coated with 2 μg/ml fibronectin (Merck, F0895). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... with 200 µl of a 2% w/v solution of sodium fluorescein in 0.9 % saline (Merck Ltd., UK). Tracer was permitted to circulate for 1 h before animals were deeply anaesthetised with sodium pentobarbital (50 mg/kg ...
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bioRxiv - Microbiology 2024Quote: ... E607063-0100) for 2 h to kill non-adherent bacteria and then lysed with 0.1% saponin (Merck; 47036). Lysates were plated on THA plates after serial dilution in PBS and bacterial numbers were counted after overnight incubation ...
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bioRxiv - Biophysics 2024Quote: ... the samples were concentrated to a volume ≤ 2 mL using Amicon® Ultra Centrifugal Filter units (Merck Millipore) and centrifuged for 5 min at 21000 xg ...
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bioRxiv - Cell Biology 2024Quote: ... LDRND25/1.5) for SLB formation were cleaned with 2% Hellmanex III solution (Hellma Analytics, cat. No. Z805939, Merck) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and the cells were then incubated with 15 μM of Cd Nitrate (Cd (NO3)2) (Merck/Sigma-Aldrich) or H2O2 (Merck/Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in LHC9/RPMI 1640 (1:1) without serum as previously described51,52 supplemented with rho-associated protein kinase 1 inhibitor (Y-27632, Merck, 5 mM), SMAD-signaling inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... TSPO-/- MPAs were grown in T25 flasks until confluent before being stored in liquid nitrogen (10% v/v dimethyl sulfoxide (DMSO; catalogue # D2650, Merck Life Sciences (UK)) until TSPO genotypes had been confirmed ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted 212Pb in 0.1 M HCl obtained from the emanation generator was adjusted to pH 5-6 with 5 M sodium acetate (Merck, Darmstadt, Germany) and mixed with TCMC-mAbs with specific activities of 1-50 MBq/mg ...
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bioRxiv - Microbiology 2021Quote: ... Methyl viologen dichloride hydrate (paraquat, 98% purity) and Isopropil-β-D-1-tiogalactopiranósido (IPTG) were purchased from Merck.
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bioRxiv - Immunology 2024Quote: ... Transformants were selected on vegetarian Yeast Extract-Peptone-Dextrose (YPD) medium supplemented with 1 M D-sorbitol (Merck) and 250 µg mL-1 Zeocin (InvivoGen) ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of the pre-settled mixture was transferred to a new 50 mL falcon tube and diluted ten times with sterile M9 mineral medium (prepared with nuclease-free water) supplemented with 0.5 mg.mL-1 D-glucose (Merck), 0.1% L-cysteine ...
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bioRxiv - Microbiology 2022Quote: ... 108 - 109 plaque-forming units (PFU) mL-1 in tryptone soya broth (TSB, Oxoid) or in quarter-strength (1/4) Ringer’s buffer (Merck) unless stated otherwise ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were permeabilised with 0.5% Triton X-100/PBS for 1 hour and blocked for 1 hour with 4% donkey serum/PBS (Merck), both at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Plant Biology 2024Quote: In some of the experiments the samples were exposed to 5 mM concentration of the phosphatase inhibitor NaF (CAS#: 7681-49-4; Merck, Darmstadt, Germany) that prevents the dephosphorylation of LHCII and therefore also the reversal of light acclimation from state 2 to state 1 (Rintamäki et al ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a mixture of the antibody constructs with a 10-fold molar excess of DFO*-NCS (5 mg/mL in DMSO) (ABX, Radeberg, Germany) was incubated ON at 4 °C with gentle shaking (Eppendorf ThermoMixer, Merck, Darmstadt, Germany). The DFO*-derivatized constructs were purified by SE-HPLC under the same conditions as described above.
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bioRxiv - Cell Biology 2024Quote: ... both untreated and treated cells at a concentration of 4-5×104/ml were placed in PBS with monochlorobimane (50 µM mCB; Merck/Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 0.8 μg/ml of 4-hydroxytamoxifen (4-OHT, Merck) was added to the culture medium for 48 hours to induce Cre-mediated excision of the promoter and first exon within the Oct4floxalleles ...
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bioRxiv - Developmental Biology 2021Quote: ... or 100 uM myosin light chain kinase inhibitor ML-7 (Merck 475880) and fixed for 20 min in 4% paraformaldehyde and 0.2% Tween 20 in PBS.
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bioRxiv - Molecular Biology 2021Quote: ... and their purity was verified with the fibroblast marker TE-7 (Merck) by immunofluorescence (IF ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2023Quote: 7-8 weeks old mice were injected intraperitoneally with azoxymethane (AOM, Merck) dissolved in isotonic saline solution at a concentration of 10 mg/kg body weight ...
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bioRxiv - Immunology 2024Quote: 7-8 weeks old mice were injected intraperitoneally with azoxymethane (AOM, Merck) dissolved in isotonic saline solution at a concentration of 10 mg/kg body weight ...