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Citations for Merck :
2101 - 2150 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Purified protein samples were concentrated to 5-6 mg ml−1 in an aqueous buffer solution (20 mM Tris, 250 mM NaCl, 20% glycerol, and 10 mM 2- mercaptoethanol) and 0.6 M Trehalose (Merck KGaA, Darmstadt, Germany) when samples were lyophilized ...
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bioRxiv - Developmental Biology 2024Quote: ... ten E6 morulae were transferred to each recipient cows (n = 2) following synchronization with initial intramuscular injection of gonadotropin-releasing hormone (Fertagyl; Merck, Rahway, NJ), standard 7-day vaginal controlled internal drug release (EAZI-BREED CIDR ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4°C with anti-KCC2 (1:200; Merck Life Sciences, Italy, #07-432) or anti-IGF-1R β (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... was cultured in high-glucose (4.5 g × ml−1) Dulbecco’s modified Eagle’s medium (DMEM) containing 4 mM stable glutamine (Merck) and supplemented with 6% inactivated fetal calf serum (iFCS ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Immunology 2020Quote: ... the cells were further cultured for 4 days in the same conditions plus Dexamethasone (1 μM; MERCK). IDCs were equally generated in 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibody anti-rabbit (CST, 1:3000) diluted in PBS containing 4% skim-milk powder (Merck) and 0.2% Tween 20 (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM ethylenediaminetetraacetic acid (EDTA)) using an ultra-centrifugal filter (Amicon ultra-4 [30 kDa]; Merck). The (His)6-tag was cleaved by reacting with HRV3C overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Biochemistry 2020Quote: ... coverslips were coated with poly-D-lysine (Merck Millipore) before seeding the cells ...
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bioRxiv - Neuroscience 2020Quote: 100 nM Cytochalasin D (Merck Calbiochem, Cat. No. 250255), 100 μM CK-666 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... were treated with adding 2μM cytochalasin D (Merck Millipore) and 0.2μM Nocodazole (Merck Millipore) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... on poly-D-lysine 50 µg/ml (Merck Millipore) and laminin 10 µg/ml (SigmaAldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... and 25µl of 2,4-D (5mg/ml, Sigma-Merck) were mixed and placed in the hole created ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... neurons were seeded on poly-D-lysine (Merck, P1149) and LAM/Laminin (Merck ...
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bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 7 prior to splitting by Ficoll-Paque Plus (Merck) step gradient centrifugation and cytotoxic T lymphocytes (CTLs ...
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bioRxiv - Microbiology 2021Quote: ... washed with 0.1 M PIPES buffer pH 7 (Merck Life Science UK Limited, Dorset, UK), and fixed with 2.5% glutaraldehyde in cacodylate buffer (Merck Life Science UK Limited ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were diluted with optically neutral quartz sand (SiO2; CAS#: 14808-60-7, Merck). For most samples the dilution series consisted of 100% (pure sample powder taken straight from the grinding) ...
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bioRxiv - Microbiology 2024Quote: We purchased α-glucosidase derived from Saccharomyces cerevisiae (CAS No: 9001-42-7) from Merck Saint Louis ...
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bioRxiv - Cancer Biology 2024Quote: ... and shRNA-expressing cells were selected for 7 days using puromycin (2.5 µg/ml, Merck) in co-culture with puromycin-resistant 3T3-J2 fibroblasts (kindly provided by Prof Fiona Watt ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 μL magnetic beads were incubated with 5 μg of Ago-1 antibody (Merck, Millipore, Germany) or IgG antibody (Merck ...
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bioRxiv - Evolutionary Biology 2023Quote: ... For removal of chorion embryos were incubated for 5 mins with 1 mg/ml Pronase (Merck) then washed with culture medium and flash-frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 mg ml-1 fibrinogen from bovine plasma type I-S (Merck KGaA, Darmstadt, Germany); then ...
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bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
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bioRxiv - Plant Biology 2022Quote: ... 13C5-5-MTHF and 13C5-5-FTHF were purchased from Merck Eprova (Schaffhausen ...
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bioRxiv - Cancer Biology 2024Quote: Single cell and/or cluster suspensions of MDA-MB 231 cells were pre-treated with Cytochalasin D (Cyto-D) (Merck Life Science, UK), or Colchicine (Colch ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Microbiology 2022Quote: The His6-eVP40-pET46 expression vector was a kind gift from Erica Ollmann Saphire (La Jolla Institute for Immunology) and was expressed and grown in RosettaTM 2 BL21 DE3 competent cells (Merck Millipore, Billerica MA). The pet28a-His6-Lact C2 bacterial expression plasmid was a kind gift from Dr ...
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bioRxiv - Microbiology 2022Quote: ... were sequentially filtered by vacuum through hydrophilic polycarbonate filters with a pore size of 20 µm (47 mm; Sterlitech, Kent, WA, US) and then 2 µm (Isopore, 47 mm; Merck Millipore, Cork, Ireland). Filters were immediately plunged into liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Genetics 2021Quote: ... Cells were differentiated by LIF withdrawal in DMEM supplemented with 10% FBS and 0.1 mM ß-Mercaptoethanol at a density of 2×104 cells/cm2 on Fibronectin-coated dishes (Merck, 10 µg/ml).
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Microbiology 2022Quote: ... medium was added to the apical side 30min prior to measurement with a chopstick electrode connected to a Volt-Ohm-meter (Millicell® ERS-2, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Cancer Biology 2024Quote: ... The gelatin mixture was prepared by dissolving aliquots of non-fluorescent gelatin (Sigma-Aldrich, Merck, G1890, dissolved in DPBS to 2 mg/mL) and FITC-conjugated gelatin (AnaSpec ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore), in a 50% dilution of blocking buffer + 0.5% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) and 20μg was immunoprecipitated overnight at 4°C with 10μg S9.6 antibody (Merck, MABE1095) conjugated to 100μL Pierce ChIP-grade protein-A/G magnetic beads (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... CMCs were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 ...
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bioRxiv - Molecular Biology 2022Quote: ... sections were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 or -568-conjugated IgG secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Choline acetyltransferase (ChAT) expression was detected with a goat polyclonal antibody (1:500; 4°C overnight, AB144p, Merck) and revealed with the secondary antibody Alexa Fluor 647 donkey anti-goat IgG (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... the scaffold was incubated overnight at 4 °C with anti-β-tubulin III antibody (1:100; Merck, Germany) and anti- synaptophysin antibody (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... fixated in 4% PFA (Merck), blocked for 30 min with 2% BSA (Affimetrix ...