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Citations for Merck :
1751 - 1773 of 1773 citations for IL 10 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... We performed gel electrophoresis on a 2% agarose gel with 10 µl 10 mg/mL ethidium bromide (EtBr) submerged in 1.5L 1X TBE with 150 µl 10 mg/mL EtBr (Sigma-Aldrich cat no. E1510; Merck KGaA, Darmstadt, Germany). Wells in the gel were loaded with 10 µl amplified DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate was concentrated down to 1.5 mL using an Amicon Ultra Centrifuge Filter unit (30 kDa cutoff for GST-UtrCH-sfGFP and 10 kDa cutoff for GST-LifeAct-sfGFP; Merck, UFC903024 and UFC901024, respectively). The GST tag was removed by digestion with 8 units/mL PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... [11] were maintained in Dulbecco’s modified Eagle medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Merck, Kenilworth, New Jersey, USA) and 1 mg/ml of G418 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were buffer exchanged into PBS and then concentrated down using Amicon® 100 kDa or 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9100 & UFC9010), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Biochemistry 2024Quote: ... Sephacryl S-200 and Sephadex G-25 (PD-10 columns) were from Amer-sham-Pharmacia (Cytiva, now purchasable from Merck Life Sciences, Madrid, Spain). DEAE-cellulose DE52 was from Whatman (now purchasable from Merck Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... the differentiating cells were transduced with mVDR and Gfi1-expressing retrovirus particles in the presence of 10 µg/mL of Polybrene transfection reagent (Merck Millipore, Burlington, MA, USA). These cells were then subsequently expanded on day 3 and finally harvested for flow cytometry on day 5 ...
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bioRxiv - Neuroscience 2020Quote: ... Infected and sham-infected animals were separated into four groups according to the treatment received: 10 μL of intramuscular vitamin B12 (Merck, Kenilworth, NJ; 6.25 mg/ kg) (N = 10 infected - vitamin B12 ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck, Sigma-Aldrich, l’Isle d’Abeau, France). After three cycles of freezing – thawing ...
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bioRxiv - Molecular Biology 2024Quote: ... the eggs were transferred into centrifuge tubes containing 1 ml XBE2 + 10 µg/ ml protease inhibitor (aprotinin Sigma A6279, leupeptin Merck 108975, pepstatin Carl Roth 2936.1) + 100 µg/ ml cytochalasin D (Sigma C8273) ...
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bioRxiv - Plant Biology 2021Quote: ... the washed seedlings were transferred into TetSee X solution (15% Na-deoxycholate, 25% urea, 10 % glycerol, 5% 2’2-thiodiethanol [Merck, Product No. 166782], 1% Triton X-100) and kept for 3 days at 4°C with daily changes of the TetSee X solution ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...