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Citations for Merck :
1351 - 1400 of 1773 citations for IL 10 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The implantation surgery was conducted under pentobarbital anesthesia (60 mg/kg, i.p.; dissolved in 0.9% sodium, 10 mg/ml, Merck, Darmstadt, Germany). The Hip and PFC recording electrodes consisted of two twisted pairs of perfluoroalkoxy (PFA)-coated stainless steel wires (diameter 0.002’’ ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were maintained in high glucose Dulbecco’s modified Eagle’s medium (HyClone, SH30243.02) supplemented with 10% EmbryoMax fetal bovine serum (Merck, ES-009-B) at 37°C with 5% CO2 and passaged every 3-4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble samples were desalted and concentrated using Amicon concentrators (10-kDa cut-off; Millipore Sigma, # UFC800308; Merck KGaA, Darmstadt, Germany). Samples (20 µg protein ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated and buffer exchanged to TE (10 mM Tris pH 7.5, 0.1 mM EDTA) using a 10,000 MWCO concentrator (Merck Pte. Ltd, Singapore).
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bioRxiv - Cell Biology 2021Quote: ... the coverslips were mounted on microscopy slides with Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Merck Millipore, 81381). The samples were imaged using a Zeiss Axioscope A1 microscope with a 40x objective.
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bioRxiv - Cell Biology 2021Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in Liberase (Merck) and 10ul/ml DNAse I (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Plant Biology 2021Quote: ... surface sterilized with 10% (v/v) bleach solution and plated on agar plates containing one-half strength Murashige and Skoog (MS) salts (Merck) pH 5.7 ...
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bioRxiv - Neuroscience 2020Quote: ... two custom-made Teflon containers of 5 mm diameter were filled with 10 μl of odor substance (n-amylacetate, AM; CAS: 628-63-7, Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting glycoconjugate was washed and concentrated with deionized water using 10 kDa centrifugal filter units (Merck Millipore, Tullagreen, Ireland).
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were spun at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... spun at 10,000 x g for 10 minutes and bacterial pellets lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: Reaction products and standards were dissolved in 20% 1-propanol and separated on a 10 cm HPTLC Si-60 plates (Merck) using 1-propanol:acetone:water 9:6:4 (v:v:v ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Microbiology 2020Quote: ... The crude fractions were dissolved in EA (10 mg/ml) and 10 µl from each were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a binary mobile phase consisting of Chloroform ...
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bioRxiv - Microbiology 2020Quote: ... Twenty µl of the EA fraction (10 mg/ml) were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a quaternary mobile phase consisting of Benzene ...
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bioRxiv - Microbiology 2020Quote: ... all supernatant was removed and the cells were fixed with 10% formalin for 15 min at room temperature and stained with 100 µg/ml Hoechst 33342 (Merck) for 30 min in the dark at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF7 cells were transduced with lentiviral particles at 75% confluency in the presence of 10 μg/ml polybrene (Merck, Germany). 24h after transduction virus containing medium was replaced with selection medium for the respective expression constructs to start the selection ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
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bioRxiv - Biophysics 2021Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 µL M2B-taxol (M2B buffer supplemented with 10 µM taxol (T7402, Merck)) ...
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bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions that were highly enriched for Redβ177 were pooled and concentrated in a 10 kDa MWCO centrifugal concentrator (Merck Millipore) to a total volume of <2 ml ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Microbiology 2022Quote: Supernatants harvested from infected Rhileki cells were centrifuged at 3,500 rpm and precipitated with 10% polyethylene glycol 8000 (Merck, Germany) overnight in 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... was repeatedly washed with PBS supplemented with protease inhibitor cocktail and reconcentrated using an Amicon® Ultra-0.5 device with 10 K MWCO (Merck) until residual urea was diluted below 10 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM stock solution was prepared by dissolving ROCKi powder in 100% dimethyl sulfoxide (DMSO, Merck Life Science, UK, #D8418) and filtered through 0.22µM syringe filters ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized for 30 minutes at room temperature in PBS supplemented with 0.2% Triton X-100 and 1% Bovine Serum Albumin (BSA) and blocked for 30 minutes at room temperature in 10% donkey serum (all from Merck). Samples were stained overnight at 4°C with the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were permeabilized with 60µL/well of 0.1% Triton X-100 for 10 minutes and stained with 50µL/well of DAPI (1:2000 dilution, in 1x PBST) (Merck, Germany) for 10 min [40] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dialyzed samples were further concentrated to 10 mg mL-1 using Amicon® Ultra-15 Centrifugal Filter Unit (Merck Millipore). The final concentration of glycerol was adjusted to 50% (v/v% ...
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bioRxiv - Microbiology 2020Quote: Stock solutions of crude extracts and fractions were prepared at a concentration of 100 mg/ml in 10% dimethyl sulfoxide (DMSO - Merck).
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) through multiple dilution and centrifugation steps using Amicon Ultra-15 centrifugal filters (10 kDa cuttoff, Merck Millipore, Germany). Protein purity was estimated with SDS-PAGE and the concentration determined with the Pierce BCA assay kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein were pooled and concentrated using Amicon Ultra-15 centrifugal filter unit (10-kDa molecular mass cut-off; Merck) until total protein concentration of 10 mg·mL−1 was reached ...
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bioRxiv - Biophysics 2019Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated by using amicon 10 k MWCO centrifugal filtration devices (Merck).
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bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... lipid extracts were also spotted on HPTLC precoated silica gel glass plates 60F254 (20×10 cm; 1.05642.0001; E. Merck, Darmstadt, Germany) using a Camag autosampler ATS4 sample applicator (Camag ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were then lysed in lysis buffer (50 mM KHPO4 pH 8, 100 mM NaCl, 10 mM Imidazole, 1X BugBuster (Merck), 25 U/ml Benzonase nuclease ...
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bioRxiv - Synthetic Biology 2019Quote: ... pH 8.5 (UA buffer) and loaded onto a 10 kDa molecular weight cut-off (MWCO) centrifugal filter (Merck Millipore, Australia) and centrifuged at 20,800 x g for 15 min at 22°C ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm: Merck) and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...