Labshake search
Citations for Merck :
1651 - 1700 of 1773 citations for IL 10 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 50 mM NaCl buffer (pH 7.0) with ultrafiltration using Amicon® Ultra-15 Centrifugal Filter Unit (10 kDa MWCO) (Merck, Darmstadt, Germany). With the prepared 100 × SYPRO® Orange solution and the protein solution ...
-
bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
-
bioRxiv - Biophysics 2019Quote: ... aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and stored at −80 °C until use ...
-
bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The tissue was processed as previously reported [26] and proteins were loaded onto 10% polyacrylamide gels and transferred to PVDF sheets (Immobilion-P, MERCK, Burlington, USA). Membranes were blocked with BSA 5% and immunoblotted using primary antibodies for β-Tubulin ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Fractions with the highest β-glucosidase activity or GFP or CFP fluorescence were pooled and applied to an Amicon Ultra centrifugal filter unit with 10 kDa cutoff (Merck Millipore, USA) for protein concentration and buffer exchange (TBS with 10 mM CaCl2 ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
-
bioRxiv - Molecular Biology 2019Quote: ... The membranes used as accumulation wall were comprised of regenerated cellulose with a molecular weight cut-off of 10 kDa (Merck Millipore DE). Flows were controlled with an Agilent Technologies 1200 series isocratic pump equipped with vacuum degasser ...
-
bioRxiv - Cell Biology 2019Quote: ... the dye was removed by buffer exchange using a spin concentrator (Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-10 membrane (Merck Millipore, UFC901024), and a buffer containing 20 mM HEPES (pH 7.4) ...
-
bioRxiv - Biophysics 2021Quote: ... aSyn was extensively dialysed against H2O and used immediately for experiments or dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and stored at −80 °C until use ...
-
bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
-
bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
-
bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was then supplemented with 5 mL of 0.9% NaCl and concentrated by centrifugation (4,000 × g; 10 min; room temperature) using Amicon Ultra-15 100 kDa centrifugal filtration units (regenerated cellulose; Merck/Millipore, Cork, Ireland). To further reduce the protein content ...
-
bioRxiv - Neuroscience 2022Quote: ... The tissue was finally washed again in PBS three times for 10 minutes before mounting using FluorSave mounting medium (Merck-Millipore #345789). For immunostainings against MBP ...
-
bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were incubated in the dark for 10 minutes before analysis by flow cytometry using the Guava® easyCyte™ 5HT HPL Benchtop flow cytometer (Merck). Data were analysed using FlowJo™ v10.2 Software (BD Life Sciences).
-
bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
-
bioRxiv - Microbiology 2022Quote: ... TBS buffer (50 mM Tris, pH 7.5, 10 mM EDTA) was filter-sterilized using a 0.22 μm filter (Merck Millipore Ltd, Burlington, MA) and kept at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
-
bioRxiv - Plant Biology 2022Quote: ... disposable 1000 μl pipette tips were fitted with 4 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1 mg:10 μg of LiChroprep® RP-18 (Merck) ...
-
bioRxiv - Plant Biology 2022Quote: ... disposable 200 μl pipette tips were fitted with 2 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1mg:10 μg of LiChroprep® RP-18 (Merck) ...
-
bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
-
bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
-
bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and 100 mM NaCl) and concentrated down to 500 mL using an Amicon® Ultra-15 10 K Centrifuge Filter (Merck Millipore).
-
bioRxiv - Microbiology 2022Quote: ... concentrated and buffer exchanged to PBS containing 0.023% DDM using a 10 kDa molecular weight cut-off centrifugal filter unit (Amicon Ultra-15, Ultracel 10K, Merck KGaA, Darmstadt, Germany) to 5 mg/ml as determined by the Bradford protein assay.
-
bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
-
bioRxiv - Cell Biology 2023Quote: ... followed by incubation on ice for 10 min and loaded on SDS-PAGE gel and wet transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Thereafter ...
-
bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
-
bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
-
bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
-
bioRxiv - Cancer Biology 2024Quote: Medium conditioned by MDA-MB-468 cells was loaded into size exclusion columns (Amicon® Ultra-15 Ultracel-10 Centrifugal Filter Unit, Merck, #UFC901024) centrifuged at 4,000g for 30 min and the fractions stored at -20⁰C until further analysis ...
-
bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
-
bioRxiv - Molecular Biology 2023Quote: ... filtered through a 0.45 µm filter and supplemented with 10 mM HEPES buffer (BI, 03-025-1B) and 12.5 µg/ml polybrene (Merck, TR-1003-G). MLV-based GFP-reporter (pNCA-GFP ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
-
bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
-
bioRxiv - Biochemistry 2023Quote: ... samples of 8 μM aSyn in the aggregation buffer were incubated at 37 °C under quiescent conditions and over 20 h with and without a cylindrical 10 mm x 3mm Teflon stirring bar (Merck, ref. Z328650) or 0.5 μM oTTR ...
-
bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
-
bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were ultracentrifuged at 13000 rpm for 10 min at 4°C and concentrated to 1.5 ml using centrifugal filters (Merck, Amicon Ultra-4). They were then exchanged into buffer #5 overnight at 4°C and aliquoted and stored at -80°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).
-
bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
-
bioRxiv - Biophysics 2023Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 μl M2B-taxol (M2B supplemented with 10 μM taxol (T7402, Merck, Darmstadt, Germany).
-
bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
-
bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...