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Citations for Merck :
1651 - 1700 of 2064 citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated and diafiltrated against 20 mM phosphate buffer containing 500 mM NaCl and 20 mM imidazole (pH 7.4) using a Labscale TFF system equipped with a 5 kDa cut-off PelliconTM XL device (Merck Millipore, PXC005C50). His-tagged trimer Spike and monomeric RBD were captured using a 5 mL HisTrap FF crude column (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 min at 37 °C and washed with 5% FBS (EmbryoMax® ES Cell Qualified FBS, ES-009-B, Merck) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... to remove larger organisms and was filtered on board first through 5 μm and then through 0.2 μm polycarbonate filters (47 mm, Merck, Darmstadt, Germany) using pressurized air ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining sample was centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2021Quote: ... they were centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Microbiology 2019Quote: ... Metabolite separation was performed by injecting 7 µL of the extract into a SeQuant® ZIC-pHILIC PEEK coated column (150 mm × 4.6 mm, 5 µm polymer, Merck Millipore) maintained at 25°C ...
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bioRxiv - Immunology 2022Quote: ... The remaining volume was spun down at 500x g for 5 minutes and resuspended in 12 ml FACS buffer (PBS [Gibco]+ 0.5% BSA [Merck] + 2mM EDTA). The suspension was transferred over a 70 μm SmartStrainer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml min−1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC system Shimadzu Prominence (Shimadzu, Kyoto, Japan) was used with Chromolith 100 (5 μm) RP-18 endcapped column (Merck, Darmstadt, Germany), mobile phase was 60% methanol ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Biophysics 2023Quote: ... were cleaned to remove any contaminants by sonication for 5 minutes in 50/50 isopropanol and Milli-Q water (Merck, Germany), followed by 5 minutes in Milli-Q only ...
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bioRxiv - Biophysics 2023Quote: Rec samples were transferred to a labeling buffer (50 mM HEPES, pH=7.0, 5 mM EGTA) using 10 kDa MWCO Amicon Ultra centrifugal filter unit (Merck Millipore, USA), and concentrated to 0.5 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... This mix was then deposited in sterilized 5 mm diameter plastic rings cut from PCR tubes (#683201, Greiner bio-one, Merck KGaA) on the surface of a chicken embryo chorioallantoic membrane ...
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bioRxiv - Bioengineering 2023Quote: ... The mixed solution was centrifuged at 4500 rpm for 5 minutes to keep the pellet which was resuspended and washed twice with 500 μL of PBS 1X (Merck, Germany) to finally add 200 μL of PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were grown until confluence in 0.2 mg/ml collagen-type I-coated 6 well plates (Merck, Darmstadt, Germany) and incubated in serum-free medium for 24 h with/without Dox treatment ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Microbiology 2022Quote: ... The AGA52 was grown in MRS broth (0.25% dextrose + 0.3% ox bile containing) supplemented with 100ppm cholesterol (5-cholesten-3β-ol (Sigma, Merck GmbH, Darmstadt, Germany), dissolved in 2-propanol ...
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bioRxiv - Genomics 2023Quote: ... 20 µL of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2x final concentration of Protease Inhibitor Cocktail Set 1 (Merck Chemicals). After centrifugation (5 min 15000g) ...
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bioRxiv - Bioengineering 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA) a constant flow rate of 0.3 ml/min with mobile phase A being 0.1 % Formic acid in 99:1 water:acetonitrile (Honeywell ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue fragments (‘organoids’) released were incubated for 5 min in Red Blood Cell Lysis buffer (Merck Sigma-Aldrich, Gillingham, Dorset, UK), washed and then plated for 1 hr at 37°C in DMEM/10%FBS (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... We performed the hybridization reaction by mixing 10 nM scaffold DNA with 15× surplus of unmodified staple strands and 30× surplus of modified staple strands in hybridization buffer (5 mM tris(hydroxymethyl)aminomethane (TRIS) (Merck, 1.08382.2500), 5 mM sodium chloride (NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV titer was quantified usizg PCR (5′-TGA GTC ACC CAC ACA AAG GA-3′ and 5′-CCA AGC TGG CCT AAC TTC AG-3′) after proteinase K treatment (Merck Millipore). Under anesthesia with a mixture of medetomidine (0.3 mg/kg ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Biophysics 2024Quote: Elution fractions containing the MS-ring composed of FliFG fusion proteins with the FliG-G214S or FliG-G215A mutations were concentrated 5-fold using an Amicon Ultra 100 K device (Merck Millipore). The G214S and G215A mutants were diluted 50- and 100-fold ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Evolutionary Biology 2024Quote: ... rinsed once again with PBS-d and then proceeded to blocking using 5% of NGS (normal goat serum, G9023, MERCK, Germany) in PBS-d for 2-4 hours ...
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bioRxiv - Microbiology 2024Quote: ... The strain was made ampicillin resistant by serial selections on Luria Broth (LB) agar (Carl Roth, Belgium) with increasing concentrations ampicillin (5 µL/mL to 100 µL/mL) (Merck, Belgium). Each time ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing Rab8-His6 were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and injected into a Superdex 75 16/60 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2.5 × 105 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 µg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Bioengineering 2020Quote: ... except that 2-monoacylglycerols were separated by thin layer chromatography (Silica gel 60, 20 × 20 cm; Sigma-Aldrich/Merck) using hexane:diethylether:acetic acid (35:70:1.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Immunology 2021Quote: ... Biochemical studies of blood serum samples were carried out on VITALAB SELECTRA 2 in an automatic analyzer (Merck, Germany) using commercial kits (DIASYS Diagnostic Systems GmbH ...