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Citations for Merck :
1351 - 1400 of 2010 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Biochemistry 2023Quote: ... and concentrated in an Amicon Ultra 10 kDa molecular weight cutoff (MWCO) centrifugal concentration device (Merck Millipore) with working buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1.2 mL of a 10 mM sodium periodate solution (Merck, cat# 7790-28-5) was added to the suspension ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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Ozone mediates tumor-selective cell death caused by air plasma-activated medium independently of NOxbioRxiv - Cancer Biology 2023Quote: ... The cells were maintained in 10% FBS (Serena Europe Gmbh, Brandenburg, Germany) containing DMEM (Merck, NM, USA) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (FBS/DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon ultra-4 centrifugation filter with a 10 kDa molecular weight cutoff (Merck Millipore), snap frozen and stored in -80 °C either directly (used for cryo-EM ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Bioengineering 2023Quote: The toxins were biotinylated using a 10:1 molar ratio of biotinylation reagent (Innolink Biotin 354S, Merck) to toxin as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Neuroscience 2023Quote: ... sections containing neurobiotin-labelled neurons were blocked with 10% Normal Donkey Serum (NDS, 566460, Merck, Darmstadt, Germany) in PBS-Tx and then incubated overnight at room temperature in darkness with Streptavidin 1:200 (Streptavidin DyLight 488 conjugated ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 macrophages on MadSurface were exposed to 10 ng/mL lipopolysaccharide (LPS, Merck Life Science NV) and 10 ng/mL recombinant human interferon-gamma (IFN-γ ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked in 10% fetal bovine serum (FBS; Cat. No. 11550356; Sigma-Aldrich, Merck, Germany) for 1 h at RT and re-stained with insulin (diluted 1:10000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 µm sections were transferred to coverslips coated with gelatin (porcine skin, 300g Bloom Type A, Merck) and stored at -80℃.
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bioRxiv - Neuroscience 2024Quote: ... and 10 heads per sample were homogenised in 100 μL 2% SDS buffer (Cat No. 428018, Merck) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS for 1 hour and probed with mouse γ/9d 2G10.2 (1:1000, MERCK MABT1335), rabbit anti mouse IgG (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... Mouse peritoneal macrophages (MPMs) were isolated from mice 4 d after the intraperitoneal injection of thioglycollate (Merck & Co.; Kenilworth, NJ, USA) as described previously(Zhang et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunofluorescence using primary polyclonal antibodies that label neurons (NeuN, mouse; 1: 1000, Millipore MAB377, Merck Millipore), reactive astrocytes (GFAP ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2019Quote: ... and mouse monoclonal anti-DNA/Histona H1 antibody (1:100; catalog number 05-457/clone AE-4; Merck Millipore, MA, EUA). Alternatively ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... were obtained from BD Transduction Laboratories (Becton Dickinson France SAS, Le Pont-De-Claix, France). Mouse monoclonal anti-α-Tubulin (ref. T6199) was purchased from Merck. Rabbit anti-Integrin β1 was a gift from the Albiges-Rizo’s lab ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked with 5% normal donkey serum for 1 h at room temperature and incubated with primary antibodies (rabbit anti-GFAP 1:200, Dako; goat anti-Iba1, 1:500 Wako; NeuroTrace™ 1:200, Thermo Fischer; mouse anti-NeuN Antibody 1:500, Merck, #MAB377 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The purity of the cultures was be routinely assessed by examining the characteristic cell morphologies under phase-contrast microscopy and confirmed by immunostaining with mouse anti-GFAP (1:200; Merck, MAB3402) and mouse anti-S100β (1:400 ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... TNF-alpha and IL-10 in serum were determined using bead-based Multi-Plex kits (MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel 96-Well Plate Assay, Merck KGaA ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... refolded H57-scFV were concentrated using Amicon®Ultra-15 centrifugal filters with a 10 kDa cutoff (Merck) and purified via gel filtration using Superdex 200 (10/300 ...
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bioRxiv - Cell Biology 2019Quote: ... TCEP was removed via 10 kDa molecular weight cut-off (MWCO) Amicon spin filters (Merck, Cat. No. UFC500324) and the nanobody was coupled through maleimide conjugation chemistry to a maleimide-DBCO crosslinker (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... washed with buffer B to stop the spheroplasting reaction and then washed into 10 % formamide (Merck Millipore S4117) in 2× SSC.
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bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
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bioRxiv - Zoology 2019Quote: ... 2017: Administered fenbendazole (50 mg/kg) for 10 days and switched from Heartgard® Plus (Merck, Kenilworth, NJ) (ivermectin/pyrantel ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μg of chromatin was immuno-precipitated with either 5 μg of CTCF antibody (07-729, Merck Millipore) or 4 μg of H3K27me3 antibody (17-622 ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing monomeric mSav*-STAR635 were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration of ~1 mg ml−1 ...