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Citations for Merck :
1451 - 1500 of 2010 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... After fixation coverslips were washed 3x 10 min with freshly made 1x PBS containing 0.1% NaBH4 (806373, Merck) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... After processing sections were mounted on microscope slides with Tris-glycerol supplemented with 10% Mowiol (Merck Millipore Calbiochem).
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bioRxiv - Bioengineering 2023Quote: ... HUVEC or HUVEC/pericyte (ratio 10:1) solutions were mixed with thrombin (4U/mL, from bovine plasma, Merck) and 20 mg/mL fibrinogen (from bovine plasma ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuging in 10 K (3 K for dA) ultra-centrifugal filters Amicon® (Merck KGaA, Darmstadt, Germany). This process was repeated five times to dialyze the ligand ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl of IP buffer and 10 μl of anti-FLAG M2 Affinity Agarose Gel (A2220, Merck Millipore) were added to the 200 μl of lysate ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Pooled fractions were buffer exchanged into 1X PBS using 10 kDa MWCO centrifugal spin concentrators (Merck, Cat#UFC801024), for 15 min at 4000 g ...
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bioRxiv - Cell Biology 2023Quote: ... Cytoskeletal drug perturbations were performed by supplementing complete media with 10 µM Blebbistatin and 100 nM Jasplakinolide (Merck) for 3-4 hours.
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bioRxiv - Biophysics 2023Quote: ... SpeMreBs with a concentration lower than the desired concentration were concentrated using an Amicon Ultra 10 K (Merck). Samples with the desired SpeMreB concentration were centrifuged (20,000×g at 4°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatographic separation of anionic phospholipid species was performed on a reverse phase C18 column (SUPELCOSIL ABZ PLUS; 10 cm x 2.1 mm, 3µm, Merck) using methanol/water (3/2 ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were maintained in Dulbecco’s modified Eagle’s medium high glucose (DMEM; D6429; Merck supplemented with 10% foetal bovine serum (FBS; F7424; Merck) and containing MycoZap plus (VZA-2012 ...
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bioRxiv - Cell Biology 2024Quote: ... 1x106 cells were aliquoted into 1.5 ml tubes and re-suspended in the DMEM supplemented with 10% FBS and 2.5% HEPES (H0887; Merck), and containing MycoZap plus ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 5 min at 2000rpm and concentrated 10 times using Amicon® Ultra 15 mL Filters (Merck #UFC910096). The concentrated virus aliquoted and stored at -80°C.
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bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Reaction was started by the addition of 10 mM Nα-Benzoyl-L-arginine ethyl ester hydrochloride (BAEE, Merck). After 30 min the reaction was quenched with EDTA (50 mM final concentration) ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 14 days in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 10 ng/ml IL-7 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then loaded on Amicon Ultra-15 centrifugal filters (10 kDa molecular weight cutoff, Merck Millipore # UFC9010) for imidazole removal and washed with purification buffer ...
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bioRxiv - Neuroscience 2023Quote: ... or Aβ-homogenate for 24 hours followed by addition of CT1812 (10 mM) or DMSO (Merck, D2438-50ML) vehicle treatment for a further 24 hours ...
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bioRxiv - Genetics 2023Quote: ... Each enzymatic reaction was stopped by adding MEMα supplemented with 10% fetal bovine serum (FBS) (Merck, Cat# S0615) and 1% Penicillin/Streptomycin (ThermoFisher scientific ...
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bioRxiv - Cell Biology 2023Quote: ... ventral side up and 10 μL of injectate mixture was injected using a Hamilton syringe (700 series – Merck) connected to a PB600 repeating syringe dispenser through the last right proleg for consistency ...
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bioRxiv - Neuroscience 2024Quote: ... followed by rinsing in 10% (wt/vol) sucrose in 0.1 M phosphate buffer containing 0.01% sodium azide (Merck) and 0.02% bacitracin (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Mice in the model groups received 10 µg of LPS (from Escherichia coli; Sigma-Aldrich, Merck Millipore, Germany) in 20 µl of sterile normal saline solution three times a week for 3.5 consecutive months ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Rgl2RA-Halo or GST- Rgl2RA/GST-BRAFRBD fusion proteins were concentrated using a concentrator (10 KDa MWCO, Merck, Amicon Ultra centrifugal filters ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the target protein were concentrated to 5 mL with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 mL of HiLoad Superdex 75 (GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with 50mM ammonium chloride for 10 min and permeabilised with 0.25% Triton X-100 (Merck) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Collected peak fractions were concentrated to 10-40 µM using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). Protein concentration was measured with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the sample was filtered in an Amicon Ultra-0.5 Centrifugal Filter Unit 10 kDa (Merck Millipore cat # UFC501096) supplemented with a 100 µl of 0.1 M Na phosphate buffer ...
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bioRxiv - Immunology 2024Quote: ... washed with pDC culture medium (low endotoxin RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... cells were briefly reactivated by addition of 10 ng/ml PMA and 1 μg/ml ionomycin (all Merck) for 1h prior to flow cytometric analysis ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescence staining of the DNA double-strand-break marker γH2AX [32] was performed using a JBW301 mouse monoclonal anti-γH2AX antibody (Merck, Darmstadt, Germany) and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.