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Citations for Merck :
1151 - 1200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... pH 7.0) containing the cOmpleteTM EDTA-Free protease inhibitor at a 1 x concentration following manufactureŕs instructions (Merck Millipore, Darmstadt, Germany). The buffer volume was proportional to the cell density of the sample ...
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bioRxiv - Microbiology 2024Quote: DDX5 KO HCT116 cells were transduced in 6-well plates using the lentiviral supernatant diluted in fresh medium supplemented with 4μg/mL of polybrene (Merck, Sigma-Aldrich). The medium was changed after 8 h and the selection with the appropriate antibiotic was initiated after 48 h ...
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bioRxiv - Microbiology 2024Quote: ... 1 tablet of commercial protease inhibitor cocktail (Merck)) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with Complete-EDTA-free Protease Inhibitor Cocktail (Merck). Cells were lysed for 30 min incubation on ice and debris were removed by 10 min centrifugation at 10,000g at 4°C ...
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bioRxiv - Microbiology 2024Quote: The chromatographic separation for metabolite profiling was performed using a SeQuant ZIC-pHILIC (2.1×100 mm, 5-μm particle) column (Merck) at 40°C ...
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bioRxiv - Microbiology 2024Quote: ... the pan-protein labelling reagent Atto-NHS ester 594 (Merck) was used (10 μg/ml in PBS ...
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bioRxiv - Microbiology 2024Quote: ... Cells were sonicated and Benzonase® Nuclease (Merck, Millipore Corp ...
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bioRxiv - Microbiology 2024Quote: ... anti-TASOR (HPA006735, lots A106822, C119001, Merck) 1/1,000 ...
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bioRxiv - Microbiology 2024Quote: ... anti-βActin (AC40, A3853, Merck) 1/1000 ...
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bioRxiv - Microbiology 2024Quote: ... anti-Flag M2 (F1804-200UG-lot SLCD3990, Merck) 1/1,000 ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5% IGEPAL® CA-630 (I8896-100ML Merck)) was added on the cell pellet and resuspended pellet was incubated on ice for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... 100 μl sample filtered through 0.1μm Ultrafree-MC centrifugal filters (Merck) was injected onto a Superdex200 10/300 GL Increase SEC column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was then exposed to Immobilon Western Chemiluminescent HRP Substrate (Merck, Darmstadt, Germany) for one min ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... and acetic acid (100%, water-free, p.a., Merck). Acetaldehyde content was further quantified using the supernatant of WT and ΔmftG grown in HdB-Tyl supplemented with 10 g L−1 ethanol alone and 10 g L−1 glucose combined with 10 g L−1 ethanol for 48 h as described in the acetaldehyde Assay Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Detection was performed via refractive index (RI) and UV (210 nm) and compared with standards of ethanol (Uvasol for spectroscopy, Merck), acetaldehyde (Reagent plus ...
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bioRxiv - Microbiology 2024Quote: ... The glycerol concentration in the medium was determined enzymatically using the recombinantly produced Saci_2033 glycerol kinase (see below) and ADP formation was quantitatively assayed spectrophotometrically as oxidation of NADH via pyruvate kinase (PK) (Merck, Germany) and lactate dehydrogenase (LDH ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg of Phosphatidylcholine (Merck, Germany) or 50 µg of isolated S ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% FBS (Sigma-Aldrich/Merck), and 2mM L-glutamine (Gibco/Thermoscientific) ...
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bioRxiv - Immunology 2024Quote: ... Spleens were minced into fine pieces using forceps and digested by incubation with 1mg/ml collagenase (Sigma-Aldrich/Merck) and 30 units/ml DNase (Roche ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Immunology 2024Quote: ... washed with pDC culture medium (low endotoxin RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... 1µM β-Estradiol (Sigma-Aldrich/Merck) and 70ng/ml recombinant Flt3L (R & D Systems ...
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... complexed to 30µg dotap (Roche/Merck) respectively ...
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bioRxiv - Immunology 2024Quote: ... viruses were harvested and concentrated using a 10K MWCO Amicon (Merck) at 1500×g ...
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bioRxiv - Microbiology 2024Quote: ... Fixation of cells with 250 µL methanol (Merck, Darmstadt, Germany) for 15 min and subsequent air-drying for 10 min followed ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
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bioRxiv - Microbiology 2024Quote: ... This suspension was diluted with 9 mL of 0.9 % NaCl and filtered through a hydrophilic polycarbonate filter (pore size 0.2 µm; Merck Millipore, Darmstadt, Germany). A segment from the center of the filter was fixed with 1 % glutaraldehyde and 4 % paraformaldehyde in washing buffer (10 mM cacodylate buffer ...
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bioRxiv - Microbiology 2024Quote: ... The LC separation was done using the SeQuant Zic-pHilic (150 mm × 2.1 mm) with the SeQuant guard column (20 mm × 2.1 mm) (Merck). The Mobile phase B was acetonitrile and Mobile phase A was 20 mM ammonium carbonate with 0.1% ammonia hydroxide in DDW ...
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bioRxiv - Immunology 2024Quote: ... and further incubated with anti IRF7 (polyclonal antibody, Merck Millipore, ABF130) or anti Myc (clone 9E10 ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Immunology 2024Quote: ... or anti Myc (clone 9E10, Merck Millipore, MABE282) antibodies ...
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bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
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bioRxiv - Immunology 2024Quote: ... RH5.2-ST) S2 cells lines were cultured in EX-CELL 420 serum-free media (Merck) supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... For reporter gene assays 293FT cells were transiently transfected using a slightly modified polyethylenimine (PEI, Sigma-Aldrich/Merck) transfection method72 ...
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bioRxiv - Immunology 2024Quote: 7-8 weeks old mice were injected intraperitoneally with azoxymethane (AOM, Merck) dissolved in isotonic saline solution at a concentration of 10 mg/kg body weight ...
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bioRxiv - Immunology 2024Quote: ... Phospho Stop (Merck), 250U/ml Benzonase and 10U/ml DNase 1 ...
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bioRxiv - Immunology 2024Quote: ... Complete EDTA free protease inhibitor (Merck), Phospho Stop (Merck) ...
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bioRxiv - Immunology 2024Quote: ... leukocytes were isolated by density gradient centrifugation using 37% Easycoll (Merck Millipore) and a filtration step using a 30-µm cell strainer (Partec) ...
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bioRxiv - Immunology 2024Quote: ... Monocrotaline and lipopolysaccharides (LPS) was purchased from Merck KGaA (Darmstadt ...
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bioRxiv - Molecular Biology 2024Quote: ... complementing the Honda Buffer with 1x of PhosSTOP (Merck). Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Trypsin was then inhibited with soybean trypsin inhibitor (Merck) at a ratio of 1 % w/w inhibitor to C3 ...
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bioRxiv - Bioengineering 2024Quote: ... Slices were put onto microscope slides with adhesive imaging spacers making wells (Merck, GBL654004-100EA), covered with PBS and with a coverslip and kept at 4°C in a dark wet chamber until they were stained for imaging ...
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bioRxiv - Bioengineering 2024Quote: ... and calcein-AM (Merck, 206700-1MG ...
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bioRxiv - Cell Biology 2024Quote: ... sorbitol or NaCl (Merck).
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bioRxiv - Microbiology 2024Quote: Parasites were treated with 50 μM of RNA Pol III inhibitor CAS 577784-91-9 (Calbiochem, Merck) after sorbitol treatment at 3 ±3hpi and RNA was harvested at 24hpi in parallel with untreated and control samples ...
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bioRxiv - Biochemistry 2024Quote: ... transferred to Microcon-30kDa Centrifugal Filter Units (MRCF0R030, Merck) and washed several times with 8 M urea and once with digestion buffer (DB ...
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bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.