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Citations for Merck :
1051 - 1100 of 2466 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A 0.1 M phosphate buffer at pH 7.2 was used and a 2% agarose gel enriched with monodisperse glass spheres (Monospher 100, Merck, Darmstadt) to simulate microscopic B0 inhomogeneities was added ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T/phoenix-AMPHO cells were selected for stable expression of retroviral vectors using Hygromycin B (200 µg/mL) and Diphtheria toxin (2 µg/mL; Merck) [16] ...
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bioRxiv - Developmental Biology 2024Quote: ... euthanized by tricaine-overdose and fixed with 2.5% glutaraldehyde (Agar Scientific, Stansted, Essex, UK) and 2% paraformaldehyde in 0.1 M Na-Cacodylate buffer (Merck, Darmstadt, Germany) with a pH of 7.44 ...
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bioRxiv - Microbiology 2024Quote: ... from the resin was immediately neutralized with 1/10 faction volume of 1 M Tris-HCl pH 8.5 and concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 100kDa NWMCO (Merck Millipore), in which the elution buffer was exchanged with PBS (GIBCO).
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2022Quote: ... Buffer was changed to 1Xpbs (Biological Industries, Israel) using multiple washes on Amicon ultra-2 columns (Merck, Burlington, MA #UFC203024).
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragmented RNA was subjected to two rounds of m6A immunoprecipitation for 2 hours each using an anti-m6A antibody (ABE572, Merck) previously conjugated to protein-A magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Biophysics 2023Quote: ... The C378A or C406A variants of 15N-pm-β2AR-Cter were labeled on the remaining cysteine using 3-(2-Iodoacetamido)-proxyl (Merck). Paramagnetic samples were recorded with a recycling delay of 2 s ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were collected and concentrated to ∼2 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All purification steps were performed at 4 °C.
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bioRxiv - Developmental Biology 2023Quote: ... 2-cell stage embryos were similarly recovered from hybrid superovulated females (using 5IU of PMSG and 5IU of hCG; Merck), generated after crossing BDF1 male and CD1 females (each strain obtained from Anlab ...
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bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Physiology 2024Quote: ... Nuclei suspension was filtered, centrifuged and resuspended in RNAse-free PBS (AM9624, ThermoFischer Scientific) with 2% Bovine Serum Albumin (A2153, Merck) and 0.2 U/µL RNAse inhibitor (3335399001 ...
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bioRxiv - Genomics 2024Quote: ... About 2 mL of supernatants were then transferred to ultrafiltration tubes (Amicon® Ultra-4 Centrifugal Filter Unit 10K, Merck). 2 mL of TE buffer was added ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were recovered by withdrawing a volume of 200 μL from the 30 – 40% interface of the OptiPrep density gradient and transferring to a new 2 mL Sorenson Dolphin microcentrifuge tube (Merck). Nuclei were washed with 2% BSA (in 1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The embryos were then incubated with anti-cleaved caspase 3 pAb (1:100) (Anti-caspase-3, cleaved (Ab-2) Rabbit pAB (PC679; Merck) followed by a wash and a second incubation with anti-rabbit 568 ...
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bioRxiv - Systems Biology 2024Quote: ... with 100 µl of a 2 % w/v solution of Evans blue tracer in 0.9 % saline (Merck Ltd, Poole, UK) or i.v ...
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bioRxiv - Neuroscience 2024Quote: ... or (2) collected 3 days after transfection and enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Viral pellets were resuspended in sterile PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM Tris (2-carboxyethyl) phosphine (TCEP)] in presence of equal molar amount of Mu217 DNA in a Pur-A-Lyzer Mini (Merck). Mu217 DNA was purified as previously described7 ...
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bioRxiv - Plant Biology 2024Quote: ... pH 5.3) and then incubated in 2% w/v pectinase from Aspegillus niger (Merck Life Science UK Ltd., Gillingham, UK) in digestion buffer at 45°C for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were removed and post-fixed for 1–2 days before transfer to 30% sucrose (Merck KGaA, Darmstadt, Germany) dissolved in PBS ...
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bioRxiv - Immunology 2024Quote: Human monocytes were plated at 2 × 105 cells/well in 96 well plates and cultured for 2-3 days in RPMI supplemented with 10% human serum (from human male AB plasma, Merck), 1 U/mL penicillin and 0.1 mg/mL streptomycin ...
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bioRxiv - Genomics 2024Quote: ... 20 mL of pre-heated (65°C) modified Cetyltrimethylammonium bromide (CTAB) extraction buffer [2% CTAB (H-5882, Merck, Darmstadt, Gerrmany); 100 mM Tris-HCl (pH8.0) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87 ...
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bioRxiv - Microbiology 2024Quote: ... The resulting concentrated retentate was then filtered through a 0.22 μm filter and added with stabilizing buffer containing 2% (v/v) gelatin hydrolysate (Merck G0262) and 5% (v/v ...
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bioRxiv - Physiology 2024Quote: Neonatal mice were euthanised by decapitation and the ovaries were dissected from the bursa and were transferred into 2 µL media droplet on a transwell insert (0.4μm pore size, Millicell, Merck, Cat# PIHP01250) floating on 0.5 mL of DMEM/F-12 media ...
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bioRxiv - Neuroscience 2024Quote: ... 0,2 ml of 60mg/ml i.p.) and brains were carefully removed and snap frozen in 2-methylbutane (Merck, Overijse, Belgium) at -40°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified viral particles (estimated amount of 10 µg) were resuspended in 500 µl PBS supplemented with 2% sodium dodecyl sulfate (SDS, Merck), pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
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bioRxiv - Cancer Biology 2024Quote: ... Dried pellet was mixed with Lysis buffer (9M urea, 4 wt% CHAPS, 2% Pharmalyte carrier ampholyte pH 3-10, Merck) and left overnight at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Calibration standards were prepared in HNO3 (2%) by diluting a certified multi-element ICP standard containing the elements of interest (Merck) to obtain a 4-point linear calibration curve ranging from 0 µg/mL to 4 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit mAb Phospho-MAPK (Erk 1/2 Thr202/Tyr204) (D13.14.4E) (1:1,000; #4370; Cell Signaling Technology, mouse mAb GAPDH (1;5000, G8795, MERCK/Sigma-Aldrich).
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bioRxiv - Bioengineering 2024Quote: Fresh macroscopically normal omental and peritoneal tissues were cut into 1-2 mm3 pieces and placed into non-adherent U-bottom 96 well plates (Merck). A solution with 10,000 cells in 10 μl media was added and cells were allowed to attach to the tissues for 2 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... treated with mitomycin C (for 2 hours at a final concentration of 10 μg/mL; purchased from Merck, NJ, US). ES cells were cultured in Glutamax Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... a concentrated lipid mixture stock solution of fatty acids conjugated to fatty acid-free BSA (10% solution in PBS) (Merck cat# A1595) was created as previously described71 ...
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bioRxiv - Bioengineering 2021Quote: ... in saturated aqueous solution of picric acid (Merck KGaA) for 1 h at RT.
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bioRxiv - Cancer Biology 2021Quote: ... and glacial acetic acid were from Merck (Darmstadt, Germany). MultiScreen® HTS 96 multiwell plates (pore size 0.45 μm ...
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bioRxiv - Molecular Biology 2021Quote: ... each sample was acidified by aqueous phosphoric acid (Merck) with the final concentration of 1.2% and then seven volumes of S-Trap binding buffer (90% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... the solution was acidified with trifluoroacetic acid (TFA; Merck) to a final concentration of 1% and a pH of ¡ 2 ...
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bioRxiv - Cell Biology 2020Quote: ... and acidified with 10% formic acid solution (Merck, #1.00264.1000). After the labeling reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... soybeans and water containing 0.05% ascorbic acid (Merck, Germany) at 25 ± 1 °C and 50 ± 5% relative humidity under a long-day regime of 16 h light and 8 h dark.
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bioRxiv - Developmental Biology 2022Quote: ... were stimulated with all-trans-Retinoic acid (R2625; Merck) at a dose of 1 or 10 μM for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with 4.7mL of 85% ortho-phosphoric acid (Merck) and filled up to 50mL with HPLC-grade water ...