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Citations for Merck :
1001 - 1050 of 2466 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Genomics 2021Quote: Cultures of three Lasiodiplodia and five Neofusicoccum species (Table 1) were inoculated onto cellophane covered 2 % malt extract agar (MEA; Biolab, Merck) and incubated at 22 °C ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... pre-cleared extracts (2 mg) were incubated for 4 hr at 4 °C with 2 μg of pan–ADP–ribose binding reagent (MABE1016, Merck) or normal rabbit IgG (2729S ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... and spleen were harvested and homogenated in PBS for CFU counting or in isotonic buffer (Tris HCl 50 nM, EDTA 2 mM, PMSF 1 mM [Roche Diagnostics GmbH], Triton X-100 1% [Merck Life Science] ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2020Quote: ... a five-day-old larva was embedded at the bottom of the central glass compartment in 2% low-gelling temperature agarose (Merck). The agarose was carefully removed around the eyes and mouth upon hardening and the arena was filled with 3 mL water ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cell Biology 2020Quote: 5×106 cells were stained with appropriate antibodies for 20min on ice in PBS + 2% FCS and analyzed on an ImageStreamX MKII imaging flow cytometer (Merck). Mfge8-eGFP+ cells were gated using the IDEAS software ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed in 500 μl of lysis buffer (8 mL of cold PBS and 2 mL of 10% Triton X-114 supplemented with Halt Protease inhibitor (1:100) and Bezonase (Merck) (1:1000)) ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Genomics 2021Quote: ... serial 10-fold dilutions were prepared from the viral stock and used to transduce mESCs in a 6-well plate (Mock plus 10-2 to 10-6) together with 8 ng/µl polybrene (Merck) in duplicates ...
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bioRxiv - Genetics 2021Quote: ... 5 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−2 to 10−6) for transduction with 8 ng/µl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Biochemistry 2021Quote: ... Lipids containing [6-3H]GlcN-PI were dissolved in 100 μL of chloroform: methanol (2:1, by volume) and loaded to a high performance thin-layer chromatography (HPTLC) plate (Merck). The [6-3H]GlcN-PI was located by phosphorimaging ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore Darmstadt, Germany), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1-oleoyl-2-acetyl-sn-glycerol (OAG) and 4α-phorbol 12,13-didecanoate (4α-PDD) were obtained from Merck (Gillingham, UK). Sphingosine-1-phosphate (S1P ...
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bioRxiv - Neuroscience 2021Quote: ... MDMi cells were incubated with a primary antibody against Iba1 (1:1,000; 019-19744, FUJIFILM Wako Chemicals) in blocking buffer (2% bovine serum albumin, 0.1% TritonX100 (Merck, Darmstadt, Germany), and 5% normal donkey serum (017-000-121 ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was eluted from the filter with 2 x 50 µl of Elution Buffer and 1 x 50 µl with DEPC-treated Molecular Biology Grade water (Merck). RNA samples were quantified with NanoDrop 2000 Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissociated follicles were kept on the Transwell-COL membranes contacted with IVG medium composed of αMEM supplemented with 2% polyvinylpyrrolidone (PVP360, Merck), 5% FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of gDNA from individual GCs (2 ng input) was performed with1 ul of JH reverse primer (10 uM, provided by MERCK) and 1.8 ul of LD forward primer set pools (10 uM per primer ...
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bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
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bioRxiv - Biochemistry 2023Quote: 500 ng – 5 µg of pre-purified rRNA subunits (20 µL of each) were digested to single nucleosides by using 2 U benzonase nuclease (> 90 % purity, Merck) 0.2 U phosphodiesterase I (VWR) ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were concentrated to 2 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 300-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Following counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Vectashield/Biozol) slices were mounted in Mowiol 4-88 (Merck Chemicals).
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bioRxiv - Plant Biology 2022Quote: Fungal elicitor mix was prepared as published previously (24, 38) and separated via enrichment using Discovery DSC-C18 (2 g) columns (Merck) with a H2O/MeOH gradient from 100 % to 0 % H2O in 10 % steps ...
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bioRxiv - Plant Biology 2024Quote: ... Ultrapure water (< 2 ppb TOC) was produced using a Milli-Q Advantage A10 water purification system (Merck, Burlington, MA, USA). For mass calibration ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were washed 3 more times with 100 µL lysis buffer 2 supplemented with 100 µg/mL polyU RNA (Merck). The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... animals at the age of 3 months were perfused using 2% Formaldehyde (Science Services, München, Germany) and 2.5% Glutaraldehyde (Merck, Darmstadt, Germany) in 0.1M Cacodylate buffer ...
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bioRxiv - Microbiology 2023Quote: ... 1 L of freshly 0.1 µm filter-sterilised seawater (n = 2) was filtered onto a 0.2 µm Sterivex (Merck Pty Ltd) filter and snap-frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Genomics 2023Quote: ... DNA was fragmented in a 2 mL low-bind round bottom Eppendorf tube using a sterile 3 mm borosilicate bead (Z143928-1EA Merck) by vortexing for 1 minute at maximum speed as described in [23] ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...
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bioRxiv - Physiology 2023Quote: ... was used to slowly inject 200 μL of the injection mix composed of ∼2×1011 genome copies of AAV diluted in phosphate-buffered saline (P4417, Merck) containing 2.5 mM MgCl2 and 1 mM KCl ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... FOs were sonicated 2 x 10 sec in ice-cold 1:2 methanol/chloroform solvent containing SPLASH LIPIDOMIX MS standard internal standard mix (Merck). 1:6 H2O was added to the samples before shaking at 1000 rpm ...