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Citations for Merck :
51 - 100 of 1388 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... FCS was mixed with 50% PEG-10,000 (Merck) at a 5:1 ratio and rotated at 4 °C for 3 h ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Genetics 2022Quote: ... Virus was concentrated using Amcion Ultra-15 30k centrifugal filter (Merck#C7715) and centrifugation for 30 min at 3,900rpm.
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-T7-tag (Merck 69522; RRID:AB_11211744; WB 1:10000) and mouse anti-PSD-95 (Thermo Fisher Scientific MA1-046 ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity tags were cleaved by incubation with either thrombin (Merck) or papain (Thermo Scientific) ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... was supplemented with 10% fetal calf serum (FCS) (Merck), 1x non-essential amino acids (NEAA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Merck, Germany). Once confluent ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% Fetal Calf Serum (FCS, #S0615, Merck), 4 mM L-glutamine (#25030-024 ...
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bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... The virus was further purified using Sepharose G100 SF resin (Merck, Darmstadt, Germany) in Econopac colums (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Virus was concentrated in PBS using Amicon Ultra-15 Centrifugal Filter Units (Merck) and stored at 4°C.31,77,78 Viral titre was quantified by inverted terminal repeat probe quantitative polymerase chain reaction (PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus- containing supernatant P1 was sterile-filtered using a 0.22 μm filter (Merck). To amplify the virus 1.5 mL P1 was added to 250 mL of Sf21 shaking cell culture at a density of 0.4*106 cells/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-myc-tag (clone 9E10, #05-419) was purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552 ...
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bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag with the 3C protease (MERCK), the protein was again subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... 100 μL of anti-mouse IgG (Fc-specific)−biotin (Merck B7401) were added and incubated for 45 min ...
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bioRxiv - Biophysics 2022Quote: ... containing 10mM HEPES (Roth) and 0.5% FCS (Biochrom GmbH, Merck Millipore). One chamber slide was used for gel expansion and the other one as non-expanded control ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of 10% FCS (fetal calf serum, #S0615, Merck) and 4 mM glutamine (#25030-024 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Bio & Sell) and 1% Penicilin/Streptomycin (Merck). Cells were grown in a humidified incubator at 37°C with 5% CO2 and regularly tested for mycoplasma contamination ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ...
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bioRxiv - Microbiology 2021Quote: ... Virus-infected culture was mixed with an equal volume of 0.4% Gelzan™ CM (Merck) and spread onto pre-warmed 0.7% Gelzan™ CM/SCV plates ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... The N-terminal tag was removed by an overnight incubation with thrombin (Merck). Undigested protein was removed by IMAC ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... and 10% heat inactivated foetal calf serum (FCS) (Merck Life Science UK) unless stated otherwise.
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Immunology 2021Quote: Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). Efficacy against steel was assessed at room temperature and 4°C ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...