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Citations for Merck :
351 - 400 of 1388 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein was concentrated using Amicon® (Merck) and stored in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 3 h at 60 V or for 30 minutes at 100 V in blotting buffer (48 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (MERCK-Millipore) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto PVDF membranes (Merck Millipore). The membranes were blocked with skim milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was transferred onto a PVDF membrane (Merck) at 60 V for 90 min in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with either Aquacide II (Merck) or Amicon® Ultra Centrifugal Filters ...
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bioRxiv - Pathology 2024Quote: ... a HA binding protein (HABP, Merck, cat.number 385911) with a biotin conjugate was used (1:200 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and incubated with the primary antibody (cofilin, Cell Signaling, cat. 5175S, 1:3000; HA-Tag, Santa Cruz, sc-7392, 1:1000; GAPDH, Merck Millipor, No. AB2302, 1:2000) for 12 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... The separated total proteins or proteins from two-dimensional gels were subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck, Cat. IPVH00010), hybridized with specific antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human Aβ mouse monoclonal antibody (clone W0-2; Merck Chemicals GmbH, Darmstadt, Germany) and rabbit anti-β-actin monoclonal antibody (clone 13E5 ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Bioengineering 2021Quote: ... Human plasminogen-depleted fibrinogen was utilized for hydrogel preparation when stated (Merck, Darmstadt, Germany). Aprotinin was purchased from R&D Systems (Minneapolis ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Mission siRNA for human Pacsin 2 siRNA (SASI_Hs01_0021-5538, SASI_Hs01_0021-5539 and SASI_Hs01_0021-5540, Merck) or MISSION siRNA Universal Negative Control #1 (SIC-001 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Calibration curve samples were generated by diluting the International Standard with human plasma (Merck) to create a standard curve ranging from 955 000 IU/mL to 95.5 IU/mL with three technical replicates of each concentration ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human kindlin2 (mouse monoclonal, 3A3, Merck, MAB2617; WB 1:1000, IF 1:200), anti-mouse kindlin2 (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... injection of 5 IU of human chorionic gonadotrophin (hCG; Pregnyl, Merck, Kilsyth, VIC, Australia) 48 h later.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a PVDF membrane (Merck), which was blocked with 5% dry milk ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Cell Biology 2019Quote: ... before protein precipitation using 10% trichloroacetic acid (TCA; Merck). After a single wash in TCA ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Neuroscience 2020Quote: ... 1:50 Hyaluronan binding protein HABP (#H0161; Merck, Germany) or 1:100 mouse anti-Neurocan (#N0913 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein precipitation with dichloromethane (Merck, Darmstadt, Germany) and methanol (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... via a BCA protein assay (Merck-Millipore cat. 71285) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein loading control tubulin (Merck, Sigma-Aldrich). Immuno-reactive complexes were detected using HRP-conjugated secondary antibodies (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Merck). The membranes were blocked with Blocking One for 30 min at room temperature and then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were concentrated using Amicon Ultracel 30K (Merck, UFC503096) according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2022Quote: ... and proteins concentrated in Amicon Ultra-15 concentrators (Merck) before flash freezing and storage at - 80 °C.
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Labeled protein bands were imaged using enhanced chemiluminescence (Merck) in combination with a Chemidoc MP Imaging System (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: Protein concentrations were determined using the Bradford (Merck, B6916) or Lowry assay (DC Protein Assay ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined via Bradford assay (Merck; #1.10306). Equal amounts of proteins (20–100μg as optimal for each antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg dephosphorylated myelin basic protein (Merck, 13-110), and purified MAK protein ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was concentrated using Amicon filters (Merck, Darmstadt, Germany) in buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...