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Citations for Merck :
451 - 500 of 1388 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred on a nitrocellulose membrane (Amersham, Merck,) which was probed over-night at 4°C with specific antibodies diluted in 1-3% skimmed milk-PBS-0.1% Tween solution ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G Sepharose®(#GE17-0618-01, Merck) was coupled to 1 μg monoclonal mouse anti-GFP antibody (#11814460001 ...
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bioRxiv - Molecular Biology 2023Quote: ... After the transfer of proteins onto a PVDF membrane (Merck), blocking was carried out (5% skimmed milk in PBS) ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of the indicated antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... 25 µl of Protein G Sepharose® (Merck, Darmstadt, Germany) were resuspended three times in TBS (0.5% NP40 ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in BugBuster Protein Extraction Reagent (Merck, Cat. No. 70584) as per vendor’s instruction and then incubated at room temperature for 15 min with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to Immobilon-P membrane (Merck Millipore Ltd.) and westerns preformed with Ift140 (23 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were reduced with 20 mM dithiothreitol (Merck Darmstadt, Germany) at 45°C for 30 minutes ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... proteins were transferred onto a PVDF membrane (Merck Millipore #IPFL00010) and blocked with 5% skimmed milk-PBST ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with Amicon Ultra centrifugal filters from Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... After transferring the proteins onto a PVDF membrane (Merck Millipore), the membranes were blocked in TBS-T (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Immunology 2023Quote: ... Separated proteins were transferred to PVDF membranes (0.45 mm, Merck) by semi-dry transfer ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were transferred into nitrocellulose membranes (Merck-Millipore, Germany) for 2h at 300 mV using an electrophoretic transfer system (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... All protein preparations were concentrated using Amicon Ultracentrifugal filters (Merck), reduced with DTT and purified by reversed-phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were supplemented with 0.5 mM cycloheximide (CHX; Merck), and the mixtures were then divided in two parts ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μL Magna ChIP™ protein G magnetic beads (Merck) per IP were washed with PBS-Tween 0.1% three times ...
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bioRxiv - Neuroscience 2023Quote: ... We determined protein concentration with Bradford reagent (Merck Life Sciences). We treated lysate samples (20 µg ...
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Microbiology 2024Quote: ... the pan-protein labelling reagent Atto-NHS ester 594 (Merck) was used (10 μg/ml in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to PVDF membranes (Immobilon-P, Merck) using wet transfer (1h 100V) ...
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bioRxiv - Cancer Biology 2024Quote: ... Chemiluminescent protein detection reagents were applied for GAPDH (Merck, GERPN2232) and laminin receptors (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were blotted on a PVDF membrane (Merck Millipore, IPVH00010). Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck) was used to measure IgG1 ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Complement components in the cellular supernatant were quantified using the MILLIPLEX MAP Human Complement Panel (Merck). The read out of the multiplex assay was performed in a Magpix instrument (Luminex).
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg/l hydrocortisone and 1 µg/l and human epidermal growth factor (hEGF) (both Merck). To determine EC sex ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... medium was further exchanged with FibroGRO™ Complete Media Kit for Culture of Human Fibroblasts (Merck), consisting of a fibroblasts-specific basal media supplemented with P/S (0.5%) ...
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney (293T) cells were maintained in the Dulbecco’s Modified Eagle Medium (D6046, Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FB-1365 ...
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bioRxiv - Bioengineering 2021Quote: The sEVs used to validate the platform were collected from a commercial human serum purchased from Merck Millipore (same lot in all preparations ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using the ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Merck).
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bioRxiv - Genomics 2022Quote: ... females and males were primed with 20 U and 10 U human chorionic gonadotropin (hCG, Pregnyl, Merck), respectively ...
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bioRxiv - Immunology 2022Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE-171440-02, Merck), collected from the interphase ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
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bioRxiv - Cell Biology 2023Quote: ... fully mature Xenopus females were injected with 260 U of human chorionic gonadotropin (hCG; Merck, Ovitrelle 250G) into the dorsal lymph sac about 12-16 h before use and kept overnight at 18 °C ...
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bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were concentrated with Amicon-Ultra-15 (Merck KGaA) to 11.2 mg ml−1 as measured by the absorbance at 280 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined by Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20 µg of proteins were separated on a NuPAGE 4-12% Bis-Tris gel (Thermo Fisher Scientific) ...