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Citations for Merck :
851 - 900 of 5379 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... mature pollen was germinated on the surface of solid PGM (18% sucrose, 0.01% H3BO3, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 (Merck), pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Immunology 2020Quote: ... rAAVDJ or rAAV6 genome plasmid and Donor plasmid at a 3:1:1 ratio in Polyethylenimine (PEI)(Merck). In total each plate was teransfected with 41,250ng of DNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and fixated for 20 min with 4% paraformaldehyde (Merck). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 (Carl Roth),125 mM KCl (Merck),1 mM MgCl2 (Merck),1 mM EGTA/KOH pH 8.0 (Carl Roth),5% glycerol (Merck),1% NP-40 (Nonidet P 40 Substitute ...
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bioRxiv - Genomics 2021Quote: ... 90 µl 5 M NaCl and 1 µl Pellet Paint (Merck) was added to each sample ...
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bioRxiv - Genetics 2021Quote: ... 90 µl 5 M NaCl and 1 µl Pellet Paint (Merck) was added to each sample ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked for 1 h with 5% skim milk (Merck) in TBST [50 mM Tris-HCL ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 cells from treated and untreated conditions were centrifuged for 5 min at 4,500 g and the pellets were resuspended in 4% paraformaldehyde (PFA, Merck, Germany), and incubated for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and were resuspended in hypotonic buffer containing 1U/µl of benzonase (Merck). Extracts were incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 U ml−1 penicillin and 50 μg ml−1 streptomycin (Merck Life Science (Sigma)) at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 6-hexanediol reagent (Sigma-Merck, 240117) were heated to 45°C and diluted to 50% in water as stock solution ...
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bioRxiv - Bioengineering 2021Quote: ... 6% Ficoll PM-400 (Merck kGaA), 0.2% Sarkosyl (Merck kGaA) ...
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bioRxiv - Cell Biology 2021Quote: ... for up to 6 days (Merck). The medium with the drug was renewed every 48 h.
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bioRxiv - Neuroscience 2019Quote: ... During all the fura-2 [Ca2+]i measurements HCSS was supplemented with 200-500 nM TPEN (Merck) to avoid interferences due to other metal ions (i.e ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1 g of the ground material was digested overnight at 90°C in a mixture of 2 ml HNO3 and 0.5 ml H2O2 (30% Suprapur, 1.07298.1000 Merck). Then 50 μl of HF (40% reagent grade ...
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bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
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bioRxiv - Biochemistry 2020Quote: ... and cultured in StemMACS iPS-Brew XF medium (Miltenyi Biotech) supplemented with 2 μM Thiazovivin (Merck Millipore), for 24 h after passaging with daily medium change ...
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bioRxiv - Biochemistry 2020Quote: ... Platelet aggregation was elicited with 2 µM of γ-thrombin (Merck, Watford, UK, final volume 200 µL). Absorbance was monitored at 430 nm every 55 s for 35 min using an Optima plate-reader (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: Intracellular Ca2+ levels were assessed in hippocampal neurons loaded with Fura-2/AM dye (Merck, Darmstadt, Germany) as previously described (Joshi ...
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bioRxiv - Immunology 2022Quote: ... The eluted fractions were cleaned and concentrated in Amicon Ultra-2 30 kDa (Merck Millipore, cat: UFC203024) by diluting them in PBS (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed another 2 times and permeabilised with 0.1% Triton™ X-100 (Merck, UK) diluted in PBS and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Physiology 2022Quote: ... was used to identify the vasa recta capillaries and anti-neural-glial 2 (NG2; AB5320, Merck-Millipore) was used as a marker of pericytes ...
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bioRxiv - Cancer Biology 2022Quote: ... Resistant cells were sorted after TROP2 induction with DOX (final concentration 2 µg/ml; purchased from Merck) by surface labeling with TROP2-specific antibodies (see further).
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bioRxiv - Immunology 2020Quote: ... and the supernatant was taken for immunoprecipitation with 2 μg of anti-ubiquitin antibody (FK2, Merck-Millipore) for 12 hours at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The nanobody was reduced using 10 mM Tris(2-carboxyethyl)phosphin -hydrochlorid (TCEP, #51805-45-9, Merck) on ice for 1h ...
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bioRxiv - Immunology 2022Quote: ... The loading pump delivered a solution of 0.1% formic acid in 98% water / 2% acetonitrile (Merck, Germany) at 10 µL/min ...
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml EDTA tubes were prepared with 100μl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany) before blood collection ...
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bioRxiv - Cancer Biology 2023Quote: ... formazan was solubilized by adding 2-propanol 0.1 M HCl (Sigma-Aldrich, Merck Life Science, Milan, Italy). The absorbance was measured at wavelength 570 nm (660 nm background wavelength ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenate was moved to 2 ml tubes and then 40% polysucrose 400 (P7798-100 g, Merck) in DPBS with calcium and magnesium was added in equal volumes for a final concentration of 20% ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM EE-analog in 150 µL of CH3OH were incubated with 2 mg/mL NaBH4 (Merck) in 150 µL of CH3OH at 37 °C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 heads per sample were homogenised in 100 μL 2% SDS buffer (Cat No. 428018, Merck) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Neuroscience 2023Quote: ... in phosphate-buffered saline (PBS) and subsequently blocked in a solution containing 2% normal goat serum (Merck) within the same permeabilization solution ...
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bioRxiv - Neuroscience 2019Quote: ... further immunolabeling was used either against Vesicular Glutamate Transporter 3 (1:2000, VGluT3; Merck, Cat#AB-5421 ...
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bioRxiv - Molecular Biology 2022Quote: ... faecalis was diluted 1:100 into 3 L of Brain heart infusion (BHI, Merck) and grown at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM DTT) and concentrated using a 3 kDa MWCO Centriprep concentrators (Merck Millipore). For best performance in the iSAT reaction ...
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).