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Citations for Merck :
701 - 750 of 3787 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Benzonase-treated controls were prepared as follows: 50 μl aliquots of soluble fraction from samples containing gDNA were supplemented with 125 U of benzonase (Merck-Millipore) and 10 mM MgCl2 and incubated at 37°C for 4 h ...
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bioRxiv - Genetics 2024Quote: ... The DNA eluate was then concentrated to ∼50 μl by centrifugation in Microcon DNA Fast Flow centrifugal filters (Merck Millipore, MRCFOR100) to achieve an average yield of 112 ng/fly ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing MCM protein were pooled and concentrated 14-fold using a 50 kDa MWCO Amicon Ultra 15 centrifugal filter (Merck, UFC905024) prior to size exclusion chromatography with a buffer containing 50 mM HEPES pH 7.8 ...
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bioRxiv - Cell Biology 2024Quote: ... washed three times with 250 µl water and reconstituted to a final concentration of 50 μg/μl with LC-MS grade water (LiChrosolv; MERCK KgaA). Samples were filled up to 100 µL with stock solutions to reach a final concentration of 2% SDS ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% glutamine and 1% penicillin/streptomycin (Merck). Fascin KD cells were selected and maintained using puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 (1:250-1:500; Merck Millipore) and RTP801 (1:100 ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL of 1:1000 fluorescein (Merck) was added to a well and incubated for 10 mins before observation ...
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bioRxiv - Neuroscience 2024Quote: ... 1-methyl-D-tryptophan (1-DMT; 1-LMT enantiomer, both Merck Life Sciences) for exploration of a possible tryptophan mechanism of immunosuppression ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: Full-length CA sequences derived from pNL4-3 was introduced to pET30a vectors (Novagen-Merck KGaA, Germany), producing a pET30a CA vector ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml Hemin (Mercury Cat#3741) and 5ul/ml vitamin K1 (Merck Cas#84-80-0)) under anaerobic conditions.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was diluted in 10 ml of 1X PBS buffer and filtered using an Amicon 50 kDa filter column (Merck, Darmstadt, Germany) to concentrate the extract to a final volume of 500 μL.
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bioRxiv - Evolutionary Biology 2022Quote: ... Soil and other large contaminants were removed by filtering the spore suspension over a 50-μm nylon mesh filter (Merck Millipore, USA). To remove small biological contaminants ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: An emulsion was created containing corn oil for DPhP and TPhP resuspension and animal exposure and 50% of corn oil from Merck (Darmstadt, Germany).
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bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... On the next day the buffer was exchanged to IEX-A buffer (25 mM Bis-Tris, 50 mM NaCl, 10 mM. β-mercaptoethanol) by ultra-filtration (Amicon Ultra-15 30 kDa, Merck Millipore) and subject to cation-exchange chromatography by a linear to IEX-B buffer (25 mM Bis-Tris ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 10 min incubation at RT with 50% (v/v) lead citrate solution in decocted H2O containing 80 mM lead citrate (Merck, Darmstadt, Germany) and 0.12 M trisodium citrate (AppliChem ...
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bioRxiv - Microbiology 2021Quote: ... 50 mM NaCl buffer (pH 7.0) with ultrafiltration using Amicon® Ultra-15 Centrifugal Filter Unit (10 kDa MWCO) (Merck, Darmstadt, Germany). With the prepared 100 × SYPRO® Orange solution and the protein solution ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were eluted using IMAC elution buffer (IMAC buffer A + 300 mM IMD) and concentrated using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany). SEC was performed as described above ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant (50 mL) was then concentrated to 5 mL by centrifugation using Amicon® Ultra-15 Ultrafiltration Centrifuge Tubes (Millipore; Merck KGaA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... TBS buffer (50 mM Tris, pH 7.5, 10 mM EDTA) was filter-sterilized using a 0.22 μm filter (Merck Millipore Ltd, Burlington, MA) and kept at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Soil and other large contaminants were removed by filtering the spore suspension over a 50-μm nylon mesh filter (Merck Millipore, USA). To remove small biological contaminants ...
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bioRxiv - Microbiology 2024Quote: ... Conjugants were purified by re-streaking to single colonies twice on SFM medium containing 50 μg/mL of hygromycin (Merck, cat# 10843555001). Spore stocks of resulting strains were eventually prepared and stored at –80°C.
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bioRxiv - Biochemistry 2024Quote: ... The fractions containing the expected monodisperse PT were pulled and concentrated to a final concentration ranging from 10 μM to 50 μM using 10000 Da MWCO Amicon ultrafiltration devices (Merck, Nottingham, UK) at 4000 g and 4 °C on a Heraeus Multifuge X1R centrifuge (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... both untreated and treated cells at a concentration of 4-5×104/ml were placed in PBS with monochlorobimane (50 µM mCB; Merck/Sigma-Aldrich) for 30 minutes ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... mouse anti tau-1 (1:200, Merck Millipore), mouse anti-c-Myc (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... RAD51 (Merck, Ab-1, PC130, dilution 1:1000), γH2AX (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Cell Biology 2021Quote: ... Measurements were run in triplicates diluting the protein sample at 50 nM concentration in NMR buffer loaded on a gasket (Grace Bio-Labs reusable CultureWellTM gaskets, Merck (#GBL103250)) mounted on a glass microscope coverslip (Marienfeld (#0107222)) ...
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bioRxiv - Biophysics 2020Quote: ... The cells were cultured in 50 ml Falcon flasks (Falcon Science, Paisley, UK) coated with EmbryoMax ultrapure water with 0.1 % gelatin (Merck Millipore, Billerica, MA, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: Equal amounts of total protein (50 μg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of total protein (50 µg) from the Hu14 depleted plasma were filtered using Amicon ultra 3kDa centrifugal filter units (MERCK, New Jersey, USA), dried in speed vac (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... was harvested and resuspended in 50 ml mineral salts medium without nitrogen source containing 100 μΜ 1,7OD (99% purity, Merck KGaA, Darmstadt, Germany) from a stock solution (75.4 mM ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were dissolved in 50 μL of Milli-Q water and filtered (0.20 μm pore size, 10 mm diameter, Merck Millipore, Burlington, MA, US)71.
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times with 200 µl/well of PBS/Tween and incubated for 10 min at room temperature with 50 µl/well of 3,3‘,5‘,5‘-tetramethylbenzidine (TMB)/H2O2 peroxidase substrate (Merck KGaA, cat. no. T4444). The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Biochemistry 2023Quote: ... and relevant fractions were pooled and concentrated to > 36 mg/ml (416 µM) using an Amicon Ultra centrifugal filter with a 50 kDa MWCO (Merck Group, Darmstadt, Germany).
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bioRxiv - Biochemistry 2023Quote: ... and diluted with SEC buffer to a final concentration of 32 mg/ml (370 µM) using an Amicon Ultra centrifugal filter with a 50 kDa MWCO (Merck Group, Darmstadt, Germany). The protein solution was used directly for protein crystallization or stored for up to 10 days on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... using reaction buffer (50 mM Tris-HCl pH 8.0, 100 mM NaCl) supplemented with 0.5 mM NADPH (Merck, Rahway, NJ, USA/Sigma-Aldrich) and 0.01-0.02 mg/mL enzyme ...
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bioRxiv - Genetics 2023Quote: ... The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter) with glass wool (Merck, KGaA, Darmstadt, Germany) and 0.2 g silica gel (High purity grade ...
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bioRxiv - Plant Biology 2022Quote: Lipid extracts from 500 mg mitochondria and 50 mg leaves were spotted onto TLC 60 plates (20 × 20 cm2, Merck KGaA, Darmstadt, Germany) in parallel with the following standards ...