Labshake search
Citations for Merck :
651 - 700 of 3787 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: Samples for native mass spectrometry were exchanged into 50 mM ammonium acetate (pH 6.8) using 10 kDa cut-off Amicon Ultra centrifugal filtration units (Merck Millipore). Samples were introduced to a first-generation Waters Synapt QToF (Waters Corporation ...
-
bioRxiv - Cell Biology 2023Quote: ... the buffer was removed and 50 μl per well of monoclonal 3F10 anti-HA-Peroxidase (Merck Life Science, Gillingham, UK) 12.5 ng per ml in washing buffer was added and the plate incubated 1 hour at room temperature ...
-
bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
-
bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
-
bioRxiv - Biophysics 2024Quote: ... Fractions with GPCR-containing nanodiscs were pooled and concentrated in 50 kDa Amicon-Ultra 0.5 mL centrifugal filters (Merck Millipore) to reach a final concentration of ∼60 μM ...
-
bioRxiv - Biochemistry 2024Quote: ... containing labeled or unlabeled glucose/glutamine and quenched by adding 50% ice-cold methanol containing 2 µg/mL cinnamic acid (Merck) as an internal control ...
-
bioRxiv - Microbiology 2024Quote: All phages in this study were isolated from wastewater according to the following procedure: 50 ml of wastewater was filtered through a 0.45 µm pore filter (Merck, Germany) to remove bacteria ...
-
bioRxiv - Microbiology 2024Quote: ... One-centimeter length of colon was homogenized (50 mg/mL) in ice-cold 50mM potassium phosphate buffer (pH 6) containing 5% hexadecyl trimethyl ammonium bromide (Merck) and hydrogen peroxide (0.0005%) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... On the day of transfection 0.4 µg total DNA is diluted in 50 µL OptiMEM per well and 1.2 µL of Xtreme HP Gene reagent added (Merck, 6366236001) before incubation at room temperature for 30 minutes before addition dropwise to wells.
-
bioRxiv - Microbiology 2024Quote: ... The resulting homogenates were serially diluted in BHI medium and plated on selective BHI-agar plates containing 50 µg/ml ampicillin (Merck). The plates were incubated overnight at 37 °C to allow the growth of ampicillin-resistant colonies that were used for CFU determination ...
-
bioRxiv - Biochemistry 2024Quote: ... for 1 h at room temperature and overnight with primary antibodies in incubating buffer (Tris-HCl, 50 mM, pH 7.5; NaCl, 200 mM; Tween-20, 0.1% and Bovine Serum Albumin (Merck, Ref A2153), 3% ...
-
bioRxiv - Genomics 2024Quote: ... The protein was analyzed by SDS-PAGE and concentrated to 100 μL by by ultrafiltration using a 50 kDa Amicon Ultra-0.5 ultrafiltration tube (Merck Millipore). Finally ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was concentrated until obtaining 50 ml of each cell line using Ultra-15 Centrifugal Filter Concentrator (Merck, Germany) and filtrated with 0.2 µm filter to eliminate larger vesicles ...
-
Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: The purified proteins were concentrated and exchanged into storage buffer (50 mM sodium phosphate buffer, 200 mM NaCl, 10% glycerol, pH 8.0) with Amicon Ultra-30K concentrator (Merck Millipore). SDS-PAGE was performed to check the protein purity and Bradford Protein Assay (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample (total homogenate or HEPES soluble fraction) was lyophilized for 12 h and digested with 50 µL of Nitric Acid (HNO3) (65% Suprapur, Merck) overnight at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... 1 μM Src inhibitor-1 (Merck), 2.5 μM Src inhibitor PP1 (Cayman Chemical ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 µM Ferrostatin-1 (Merck, #SML0583), 10 µM oleic acid (Merck ...
-
bioRxiv - Microbiology 2023Quote: ... 1 g.L-1 L-cysteine (Merck) and 1 g.L-1 NaHCO3 (Carl Roth GmbH ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and (iv) trace elements : 0.4 mM Na2EDTA·2H2O (Merck/Sigma-Aldrich, CAS number : 6381-92-6), 0.03 mM FeCl3·6H2O (Merck/Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-HES-1 (1:500, 1% casein, #AB5702, Millipore, Merck, Darmstadt, Germany), anti-LRP6 (1:1000 ...
-
bioRxiv - Developmental Biology 2020Quote: ... The aspirated follicular fluid was transferred to a 50 ml falcon tube containing 140 µl of 5000 IU/ml Heparin (Merck Millipore). COCs were allowed to sediment and then washed extensively with pre-warmed TCM199 (HEPES-buffered medium 199 ...
-
bioRxiv - Genomics 2022Quote: ... in 3×250 ml bottles and resuspended in 100 ml prewarmed 30°C YPD supplemented with 50 ug/ml Pronase (Merck 10165921001), at which point the count-up for the time course was initiated ...
-
bioRxiv - Immunology 2021Quote: ... Color was developed in the dark for 5-10 min before adding 50 µL of 2 M H2SO4 stop solution (Merck, Germany). Optical density was read at wavelength 450 nm using the microplate reader (SpectraMax ID3 ...
-
bioRxiv - Biochemistry 2020Quote: ... the original 8 μM 655-QDs suspension in borate buffer (50 mM borate) from the supplier was pipetted into 1.5 mL fresh ul-trapure water (Merck Millipore, US) of varying concentrations of KCl (Chem-supply ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were diluted with Milli-Q water (18.2 MΩ cm) to 50 mL and syringe filtered to <0.2 μm (Merck-Millipore, Burlington, USA) prior to analysis by inductively coupled plasma mass spectrometry (icapQ model ...
-
bioRxiv - Plant Biology 2020Quote: ... tumefaciens were resuspended in 50 mM MES (2-Morpholinoethanesulfonic acid hydrate) (Duchefa, Haarlem, The Netherlands) - KOH buffer (pH 5.6) containing 2mM NaH2PO4 (Merck, Darmstadt, Germany), 100 µM acetosyringone (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... concentrated to 33 mg/ml using an Amicon Ultra 50 ml 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) and then aliquoted before flash freezing the protein sample in liquid nitrogen ...
-
A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and concentrated using Amicon concentrator devices (molecular weight cut-off, 10 000 and for full-length protein 50 000; Merck Millipore). Recombinant His-tagged proteins (MA4376 ...
-
bioRxiv - Cell Biology 2021Quote: ... method [68] with the following modification: Equal volumes of spent media were concentrated to approximately 50 μL with Microcon - 10 kDa centrifugal filter units (Merck Millipore) at a speed of 14,000 x g ...
-
bioRxiv - Molecular Biology 2021Quote: ... The fractions corresponding to the elution peak were selected and concentrated to 2-50 mg/mL through the use of Amicon® Ultra-15 Centrifugal Unit (Merck), frozen and stored at −80°C for downstream experiments ...
-
bioRxiv - Molecular Biology 2022Quote: ... Extracted circularised cDNA in the water phase (~50 μl) was further gel-filtered using 1x HE-equilibrated Illustra MicroSpin G-25 Columns (Sigma-Aldrich/Merck) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
-
bioRxiv - Molecular Biology 2022Quote: ... washed three times with 250 µl water and reconstituted to a final concentration of 50 μg/μl with LC-MS grade water (LiChrosolv; MERCK KgaA). Samples were filled up to 100 µL with stock solutions to reach a final concentration of 2% SDS ...
-
bioRxiv - Genetics 2023Quote: Chemical extractions of single wasps were performed by immersing them in 50 µl HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) in 2 ml glass vials (Agilent Technologies ...
-
bioRxiv - Biophysics 2023Quote: Rec samples were transferred to a labeling buffer (50 mM HEPES, pH=7.0, 5 mM EGTA) using 10 kDa MWCO Amicon Ultra centrifugal filter unit (Merck Millipore, USA), and concentrated to 0.5 μg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
-
bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer exchanged with SnakeSkin™ dialysis tubing (10 MWCO, Pierce, 68100) followed by additional dialysis and concentration with Amicon Ultra-15 50 kDa centrifugal units (Merck, UFC905008). Purified protein was conjugated to AlexaFluor647 (Thermo Fisher ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting concentrate was applied to a 10 - 50% v/v iodixanol step gradient with 10% increments (OptiPrep; Sigma Aldrich, Merck) enriched with sea salts (Sigma Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
-
bioRxiv - Cancer Biology 2023Quote: ... washed three times with 250 μl water and reconstituted to a final concentration of 50 μg/μl with LC-MS grade water (LiChrosolv; MERCK KgaA). Samples were filled up to 100 μL with stock solutions to reach a final concentration of 2% SDS ...
-
bioRxiv - Cell Biology 2023Quote: ... washed three times with 250 µl water and reconstituted to a final concentration of 50 μg/μl with LC-MS grade water (LiChrosolv; MERCK KgaA). Samples (∼100 µL ...
-
bioRxiv - Cell Biology 2023Quote: ... for 15 min at RT and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2h at RT in the dark ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell pellets were then washed in cold 50 mL cold PBS cOmplete™ EDTA-free Protease Inhibitor Cocktail tablets (Merck, #73567200) and centrifuged at 2000 rpm for a further 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... 200μL for 0.5% glucose added or 250μL for 0.4% glucose) of final expression cultures were centrifuged and pellets were lysed with 50 μL bugbuster (Merck, Rahway, NJ). After centrifugation ...
-
bioRxiv - Synthetic Biology 2024Quote: ... three washing steps were performed with Millipore water using Amicon Ultra-0.5 centrifugal filters 50 kDa MWCO (Merck KGaA, Darmstadt, Germany). Concentration of final stock solutions (ranging from 170 to 300 g/l ...
-
bioRxiv - Microbiology 2024Quote: ... Benzonase-treated controls were prepared as follows: 50 μl aliquots of soluble fraction from samples containing gDNA were supplemented with 125 U of benzonase (Merck-Millipore) and 10 mM MgCl2 and incubated at 37°C for 4 h ...
-
bioRxiv - Bioengineering 2024Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer56 for storage at −80℃.