Labshake search
Citations for Merck :
601 - 650 of 3787 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Gel lanes were cut into seven gel pieces and destained with 50 mM TriEthylAmmonium BiCarbonate (TEABC, Merck, Ref T7408) followed by three washes in 100% acetonitrile ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 µg/sample or the complete fraction were filtered using an Amicon Ultra Centrifugation filter 50 kDa MWCO (Merck). The material retained on the filter was resuspended in 300 µl SEC-Buffer (1 M NaCl ...
-
bioRxiv - Genomics 2024Quote: ... ratio by a Gibson approach with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Immunology 2024Quote: ... Smith 40 using reverse-phase HPLC on a 4.6 mm internal diameter× 50 mm long reversed-phase C18 HPLC column (Chromolith Speed Rod, Merck) using an AKTAmicro HPLC system (GE Healthcare) ...
-
bioRxiv - Genomics 2024Quote: ... and ligated by Gibson with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Biochemistry 2024Quote: ... were lysed in 2 ml lysis buffer (0.5% IGEPAL, 100 mM KCl, 50 mM Tris pH 7.5, 5 mM MgCl2, with protease inhibitors (Merck, 4693159001)) ...
-
bioRxiv - Cell Biology 2024Quote: ... stimulator cells (0.5 × 106 cells per well) were treated with mitomycin C (50 μg/mL; Sigma-Aldrich; Merck Millipore) for 30 min at 37 °C in a 24-well plate ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were lysed in 500 μl lysis buffer (0.5% IGEPAL, 150 mM NaCl, 50 mM Tris-HCl pH 7.5, 5 mM MgCl2, protease inhibitors (Merck, 4693159001) and centrifugation 14,500 × g ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were then lysed in 30 μl lysis buffer (0.5% IGEPAL, 150 mM NaCl, 50 mM Tris-HCl pH 7.5, 5 mM MgCl2, protease inhibitors (Merck, 4693159001) before centrifugation 14,500 × g ...
-
bioRxiv - Biochemistry 2024Quote: ... the cell pellet was thawed in an equal volume of 2X lysis buffer (400 mM NaCl, 50 mM Tris pH 7.5, 0.5 mM DTT, 17 mg/mL Phenylmethanesulfonyl Fluoride (Merck #P7626), 25 μg/mL Leupeptin (Merck #2884) ...
-
bioRxiv - Microbiology 2022Quote: ... α: β: γ: δ = 1:1:1:1 was obtained from Merck (Darmstadt, Germany).
-
bioRxiv - Physiology 2022Quote: ... Food contained low melting agar (OmniPur® Agarose, Low Melting, CAS 9012-36-6, Merck) during lifespan assessments only to ensure the quality of compounds was preserved ...
-
bioRxiv - Neuroscience 2022Quote: ... Agarose 0.6% brain phantoms50 were prepared by dissolving agarose (A9539, CAS 9012-36-6, Merck) in tris-borate-EDTA buffer 1X (T4415 ...
-
bioRxiv - Neuroscience 2022Quote: ... The additive was prepared by dissolving D-Glucose-13C6 (389374, CAS 110187-42-3, Merck) as internal standard (IS ...
-
bioRxiv - Cell Biology 2023Quote: ... Culture dishes and microscopy dishes were coated with 0.1% gelatin (CAS 9000-70-8, Merck) in phosphate-buffered saline 30 min prior to cell seeding ...
-
bioRxiv - Plant Biology 2024Quote: ... the samples were diluted with optically neutral quartz sand (SiO2; CAS#: 14808-60-7, Merck). For most samples the dilution series consisted of 100% (pure sample powder taken straight from the grinding) ...
-
bioRxiv - Microbiology 2024Quote: We purchased α-glucosidase derived from Saccharomyces cerevisiae (CAS No: 9001-42-7) from Merck Saint Louis ...
-
bioRxiv - Biophysics 2024Quote: ... 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC, CAS: 816-94-4) was sourced from Merck KGaA (Darmstadt ...
-
bioRxiv - Developmental Biology 2024Quote: ... at room temperature for 1 hour and incubated with primary antibodies (KLF4, 1:500, Proteintech, 11880-1-AP; KLF5, 1:500, Proteintech, 21017-1-AP; SOX9, 1:600, Merck, AB5535 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The 40S- and 60S-subunit fractions were mixed and concentrated using Amicon Ultra filter units (MWCO 50 kDa, Merck Millipore). The concentrated mixture was loaded onto a 10–50% (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 ml of pre-warmed 2xSSC with or without RNAseA (50 μg/ml final concentration, Merck) and incubated 1h30 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
-
bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...
-
bioRxiv - Cell Biology 2021Quote: ... HL-60 and MS-5 cells cultured alone and in co-culture were incubated for 24 hours in 50 μM H2O2 (Merck) complete cell culture medium ...
-
bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
-
bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing MbxA were pooled and concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore). Histidine was removed with a PD10 desalting column (GE Healthcare ...
-
bioRxiv - Genomics 2022Quote: ... both isolates were grown on SAB agar media in tissue culture flasks to minimise cross-contamination and spores were harvested in PBS/Tween20 and transferred to 2 ml screw top tubes containing 425–600 mm washed glass beads (filled to the 300 mL mark; ~50 mg) (Merck). Spores were centrifuged at max speed for 2 min using a benchtop centrifuge and the supernatant was removed ...
-
bioRxiv - Bioengineering 2022Quote: ... the samples were transferred into 50 mL conical tubes with 20 mL of SM buffer (50 mM Tris HCl, 100 mM NaCl, 8 mM MgSO4, pH 7.5, Merck, Germany) and vortexed for 60 s to remove phage Φ6 particles attached to sample surfaces ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
-
bioRxiv - Microbiology 2020Quote: ... A total of 50 μg of protein from each sample was transferred to a protein LoBind plate (Merck, NJ, USA). Protein was reduced with tris (2-carboxyethyl ...
-
bioRxiv - Biophysics 2021Quote: ... and buffer exchanged into 50 mM trisaminomethane-hydrochloride (Tris-HCl) at pH 7.4 using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Aliquots of our working standard were generated by spiking 50 μL of our generated VLPs into 950 μL of human plasma (Merck). The calibration curve samples and the aliquots of working standard were then tested using a cobas® 6800 system (Roche ...
-
Biodiversity and Antimicrobial Potential of Bacterial Endophytes from Halophyte Salicornia brachiatabioRxiv - Microbiology 2020Quote: Purification of compounds was carried out by separating 50 mg of crude extract on preparative TLC plates (Kieselgel 60 F254, 25 mm, Merck) using the same solvent system as above ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS and lysed for 5 min on ice in 50 μL RIPA buffer supplemented with protease inhibitors (Protease Inhibitor Cocktail set III, EDTA free, Merck) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Proteins from the flow through were pooled and the buffer was exchanged to 25 mM KPi (pH 7.5) by ultrafiltration using a 50 kDa Amicon Ultra centrifugal filter (Merck-Millipore), and then concentrated to 5 mL ...
-
bioRxiv - Microbiology 2020Quote: ... Purified Vag8 was concentrated and the buffer was exchanged to buffer F (50 mM Tris-HCl pH 8.0, 150 mM NaCl) by ultrafiltration (Amicon Ultra, Merck-Millipore).
-
bioRxiv - Neuroscience 2022Quote: ... cells were replated onto coverglass (8×104 per well) coated with 50 µg/ml Poly-D-Lysine (PDL, Merck, USA) for imaging experiments.
-
bioRxiv - Evolutionary Biology 2022Quote: ... Hyporheic water samples of 250–500 ml were filtered using a 50-ml sterile syringe (Terumo) and 0.22-μm Sterivex™ filters (Merck Millipore ...
-
bioRxiv - Neuroscience 2022Quote: ... and placed in a T75 flask (pre-coated with 50 μg/mL poly-D-lysine (Merck Millipore, A-003-E) for 1 hour) ...
-
bioRxiv - Molecular Biology 2022Quote: ... LV tissue was solubilized in 50 mM Tris-SDS buffer (pH 6.8) containing 8 µg/mL leupeptin (Merck Life Science) and phosphatase inhibitor cocktail (Merck Life Science) ...
-
bioRxiv - Neuroscience 2024Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... was filtered through a 0.45 μm syringe filter and 50-60× concentrated using Amicon Ultra-15 100 kDa NMWCO columns (#UFC910024, Merck Millipore). The resulting concentrate was aliquoted and stored at -80°C until further use.
-
bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
-
bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
-
bioRxiv - Neuroscience 2023Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... The pellet was subjected to three freeze-thaw cycles and incubated with SAN (Merck, 50 U per 15 cm dish) for 1 h at 37 C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...
-
bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
-
bioRxiv - Biophysics 2023Quote: ... Peak fractions were collected and concentrated to ∼2 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All purification steps were performed at 4 °C.