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Citations for Merck :
701 - 750 of 6118 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Proteins were separated by SDS-PAGE using 4-12% Bis-Tris gels (Merck, #MP41G12) and transferred onto an activated PVDF membrane (Merck ...
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bioRxiv - Immunology 2024Quote: Sections were counterstained for Epcam by overnight incubation at 4°C with HPA026761 (Merck) rabbit primary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... coated with 4 uL of Engelbreth-Holm-Swarm murine sarcoma ECM gel (E1270, Merck). Muscle fibers were kept in α-MEM containing 5% fetal bovine serum in a cell incubator (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... followed by the same volume of ice-cold 4% paraformaldehyde (PFA) (1.04005.1000, Merck Millipore) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Neuroscience 2024Quote: ... followed by the same volume of ice-cold 4% paraformaldehyde (PFA) (1.04005.1000, Merck Millipore) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Cancer Biology 2024Quote: ... For Colony Formation Assay the cells were fixed with 4% paraformaldehyde (Merck Millipore #104002) diluted in PBS and stained with 2% crystal violet (Sigma-Aldrich #HT90132 ...
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bioRxiv - Neuroscience 2024Quote: ... human cerebral organoids were fixed for 60 min in 4% PFA (MC1040051000, Merck, Germany). Afterwards the tissues were incubated in 30% sucrose in DPBS (14190-094 ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions containing α-synuclein were concentrated (Amicon® Ultra 4 MWCo 3kD, Merck Millipore) and subjected to size exclusion chromatography (Superdex™ 200 Increase 10/300 ...
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bioRxiv - Developmental Biology 2022Quote: ... Rabbit Aquaporin 5 (Merck, 1:200), Rabbit ERG (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mM 3-isobutyl-1-methylxanthine (Merck, I5879), 1 μM dexamethasone (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of Benzonase (Merck Millipore, US170664-3) was added and samples left on ice until an aqueous solution formed (30 min to 1 h) ...
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bioRxiv - Neuroscience 2024Quote: ... and 3-NT (1:1000, 06-284, Merck) were used ...
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bioRxiv - Neuroscience 2024Quote: ... or 3-NT (1:1000; 06-284, Merck). Following primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with DPBS for 5 minutes and incubated for 1 hour with respective secondary antibodies (Suppl. table 3) and DAPI (#10236276001; Merck Life Science, Dorset, UK) for nuclear counterstaining in 1% donkey serum ...
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bioRxiv - Molecular Biology 2022Quote: ... cathepsin inhibitors: Pepstatin A methyl ester (10 or 20 µM, Merck, US1516485-1MG) or E64d (20 µM ...
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bioRxiv - Immunology 2022Quote: ... The cell suspensions were supplemented with 0,25% methyl cellulose (cat. #M7027, Merck KGaA), and with either BMMCs in RPMI medium (with 20 ng/ml rm IL-3 and 20 ng/ml rm SCF ...
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bioRxiv - Neuroscience 2023Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay was used to analyze the cell viability of SH-SY5Y cells ...
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bioRxiv - Microbiology 2024Quote: ... 2–3 mL of stock solution was applied to preparative TLC plates (glass, Merck, 20x20 cm ...
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bioRxiv - Cancer Biology 2024Quote: The 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Merck Sigma, Burlington, MA) reduction assay was used to quantify cell proliferation ...
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bioRxiv - Neuroscience 2024Quote: ... xylene (2 x 5 min) and coverslipped using Entallan mounting medium (Merck). Samples were imaged using an Olympus BX51 microscope equipped with an Olympus DP27 digital camera (Olympus Microscope Solutions).
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bioRxiv - Cancer Biology 2024Quote: ... 2 nM T3 supplement (3,3′,5-triiodo-l-thyronine sodium salt) (Merck), and 100 IU/mL penicillin and 100 µg/mL streptomycin (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... MRS 2211 (2-[(2-chloro-5-nitrophenyl)azo]-5-hydroxy-6- methyl-3-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde disodium salt and MRS 2395 (2,2-dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9- yl)-2-(2,2-dimethyl- propionyloxymethyl)-propyl ester) were obtained from Merck (Darmstadt, Germany).
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bioRxiv - Neuroscience 2021Quote: ... we embedded the brains in 3-5 % oxidized agarose (Type-I agarose, Merck KGaA, Germany) and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4 ...
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bioRxiv - Neuroscience 2022Quote: ... we embedded the brains in 3-5 % oxidized agarose (Type-I agarose, Merck KGaA, Germany) and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4 ...
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bioRxiv - Microbiology 2024Quote: ... polyethylene glycol was added to a final concentration of 5% (Cat # 25322-68-3, Merck), and a LFA strip (Cat # 3822-9000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase, Merck, dissolved in TIRF buffer), 45% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The gelatinous sack surrounding the eggs was removed using 4% L-Cysteine (Merck Milipore, USA) and followed by microinjecting the zygotes with Morpholino antisense oligonucleotide (MO) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells fixed for 10 min with 4% paraformaldehyde (pH 7.0; prepared from paraformaldehyde powder (Merck) by heating in PBS up to 60 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The recovered nanoparticles were pooled in Amicon Ultra-4 100 kDa centrifugal filter units (Merck) and concentrated by centrifuging at 2000 ×g until the lipid concentration was increased to 30–40 mM.
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bioRxiv - Neuroscience 2020Quote: ... Mowiol® 4-88 used for mounting medium was purchased from Merck (Kenilworth, MJ, USA). Cell culture reagents – Ham’s-F12 ...
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bioRxiv - Biochemistry 2021Quote: ... The purified complex I was concentrated using an Amicon Ultra-4 100K centrifugal filter (Merck) to 0.5 mg mL-1 (w/v) ...
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bioRxiv - Biochemistry 2022Quote: ... After the standard procedure of Ni-NTA purification (Ni-NTA beads, Merck, cat. 70666-4) and Sumo protease cleavage (in-house production ...
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bioRxiv - Biochemistry 2022Quote: ... 4 % glycerol) freshly supplemented with 5µg/ml Poly(dI-dC) (Merck Life Science cat. P4929) and 0.5 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The active fraction was concentrated using a 50 kDa cutoff filter Amicon Ultra-4 (Merck), and of 20 μL was mixed with 2 μL of 5.9 mM coelenterazine and 278 μL of 20 mM Bis-Tris-HCl (pH 7.0) ...
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... General histology was assessed after tissue fixation with 4% paraformaldehyde by H&E staining (Merck) or Picro Sirius Red stain (Direct Red 80 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for ChIP were anti-H3K4me3 (Merck Millipore, 04–745, 4 μl per ChIP), anti-H3K27me3 (Merck Millipore ...
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bioRxiv - Pathology 2020Quote: ... the harvested tissues were immediately fixed in 4% (w/v) paraformaldehyde (PFA; Merck, Darmstadt, Germany) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... 21,000 x g at 4°C and sterile-filtered using a 0.45µm PVDF-membrane (Merck).
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bioRxiv - Cell Biology 2023Quote: ... sections were rehydrated and stained with haematoxylin (Fluka) for 4 min and with eosin (Merck) for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% X-Gal (from a 4% X-Gal solution in dimethylformamide, Merck Life science). Buffer was passed through a 0.2 micron syringe filter before X-Gal was added ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were stained with Ponceau S and subsequently blocked in 4% BSA (A8022, Sigma/Merck) in 1x PBS-Tween (137 mM NACl ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µM trans- Epoxysuccinyl-L-leucylamido(4-guanidino)butan (E64, Sigma-Aldrich, Merck, Taufkirchen, Germany) was added ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 30 min at 4°C with 0.4 % paraformaldehyde (PFA, Merck KGaA) and permeabilized by washing twice with permeabilization buffer (PBS containing 2 % FBS and 0.1 % saponin (Sigma)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Rad54 was concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then concentrated through an Amicon Ultra-4 Filter Unit (Merck, cat#UFC8100214). The validation of the cytoplasm fraction was performed by western blot using antibodies against markers for nucleus (H2A) ...