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Citations for Merck :
6351 - 6398 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Bioengineering 2024Quote: ... The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm2/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was then sheared either by passaging 10 times through a 27G needle attached to a 1 ml syringe or by the addition of Benzonase® Nuclease (Merck, UK). Needle-sheared nuclear fractions were centrifuged at 13,000 x g for 10 minutes (4°C ...
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bioRxiv - Biophysics 2024Quote: ... The membrane pellet was resuspended in extraction buffer (25mM MOPS-NaOH, 150mM NaCl, 50mM EDTA, 10mM DTT, 0.1 mg/ml lysozyme, 1% lauroylsarcosine (Merck, Cat. No. L5777), pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Synthetic Biology 2021Quote: ... the diet for both the transgenic and wild-type strains was supplemented with CuSO4•5H2O (1 mM, Merck, CAS-no: 7758-99-9). Flies were reared in a controlled environment room at 25.0 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... was washed three times in Tris-buffer (pH 7.48) and resuspended in 1 %w/v Pluronic® F-127 (Merck Chemicals GmbH, Germany) Tris-Buffer.
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Biochemistry 2024Quote: ... CHAPS was added to the dialyzed sample to concentration of 1% after which the MSP was concentrated to 20-40 mg/ml in centrifugal filter (Merck, MWCO 30 kDa), flash frozen and stored at -80°C.
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Biochemistry 2022Quote: ... was added and incubated for 1 min at room temperature with gentle shaking (100 mM stock was prepared from fresh H2O2 Merck 30% w/w).
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Biochemistry 2024Quote: ... The QconCAT was precipitated and resuspended in 30 μL 25 mM ammonium bicarbonate with 0.1% (w/v) RapiGest™ SF surfactant (Waters, UK) and protease inhibitors (Roche cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Merck, UK).
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bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
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bioRxiv - Plant Biology 2024Quote: ... or standards (gallic acid in the range of 0–80 µg ml-1) were mixed with 200 µl of 10% Folin-Ciocalteu’s phenol reagent (Sigma-Aldrich, Merck, Burlington MA, USA) and incubated at 25°C in the dark for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Purity of our mitotic sample was confirmed in a small sample cytospined (Simport, #720-1962) and immunostained with anti-pHH3 (Merck, #06-570, 1:500) and DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Microbiology 2021Quote: Colorimetric pH measurement was performed for 12 independent pancreatic juice samples by applying 1 µL sample onto MColorpHast™ pH strips (Merck Millipore, MA, USA). To adjust the pH of pancreatic juice ...
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bioRxiv - Cancer Biology 2021Quote: ... Puromycin incorporation is a proxy for the mRNA translation rate in vitro and was measured by western blotting using an anti-puromycin antibody (MABE343, clone 12D10, Merck Millipore, 1:10 000). The antibody was diluted in Milk/TBST (5% non-fat dry milk in TBS with 0.1% Tween20) ...
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bioRxiv - Cancer Biology 2024Quote: OncoTICE® vials were resuspended in sterile sodium chloride 0.9% solution at the Day Hospital (Champalimaud Foundation) according to the manufacturer instructions (Merck: 1 vial/50mL saline solution). Remnants from resuspended vials were stored at 4°C and protected from light.
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bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
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bioRxiv - Microbiology 2024Quote: To levels of cytokines in the sera of infected animals were obtained using the MILLIPLEX® MAP Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay Kit (Merck-Millipore, BCYT1-33K) and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled fractions were concentrated to 20 mg mL-1 using a 10-kDa cut off filtration membrane (Cat. UFC501096, Merck Millipore, Burlington, MA, USA). Sitting drop crystallization trials were set up by depositing 150 nL of the precipitant solution on top of 150 nL of protein with 70 μL of the reservoir using a Crystal Gryphon LCP robot (Art Robbins Instruments) ...
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bioRxiv - Developmental Biology 2021Quote: ... The calcium-free ringer solution was replaced with 1 mL of protease solution (72 μg/mL Liberase DH research grade from Merck/Sigma, 0.4U/mL DNaseI-Invitrogen). The embryos were incubated at 28.5C on an orbital shaker for 20 minutes ...
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bioRxiv - Paleontology 2023Quote: An area of 1 cm² from bone artifacts was rubbed with a small swab (Foamtec UltraSOLV™ 1700 Series Swabs, Merck Life Science S.A.S, France) on a flat section of the artifact’s surface ...
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bioRxiv - Molecular Biology 2024Quote: ... the eggs were transferred into centrifuge tubes containing 1 ml XBE2 + 10 µg/ ml protease inhibitor (aprotinin Sigma A6279, leupeptin Merck 108975, pepstatin Carl Roth 2936.1) + 100 µg/ ml cytochalasin D (Sigma C8273) ...
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bioRxiv - Microbiology 2024Quote: ... were coated the day before with rabbit anti-mouse IgM antibody (100 μL/well, 1:500 dilution, SAB3701197, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... Migrated PBMCs were further processed according to the manufacturers protocol and stained with CyQuant GR Dye diluted at 1/75 (Merck, Sigma-Aldrich, Cat. No. ECM512). Fluorescence was measured at 480 ± 15 nm (excitation ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... non-cholesterol precursor sterols and 5a-cholestanol, 50 μg 5α-cholestane (Serva, Heidelberg, Germany) (50 μl from a stock solution of 5α-cholestane in cyclohexane [Merck KGaA, Darmstadt, Germany]; 1 mg/ml), and one μg epicoprostanol (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA, Merck; 0.1% SDS, to pH 7.6 with Tris) at 25 mA for 1.5 h ...