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Citations for Merck :
5951 - 6000 of 6398 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: Tear samples were collected successively from both eyes for 1 minute each using 1µL microcapillary glass tubes (Merck, Drummond Scientific ...
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bioRxiv - Immunology 2024Quote: ... before staining on ice in the dark for 1 h with mouse monoclonal anti-Puromycin-AF647 (Merck Millipore), diluted in the same solution ...
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bioRxiv - Molecular Biology 2024Quote: ... and then incubated with pre-equilibrated protein G agarose plus anti-FLAG M2 (F3165, Merck, 1 μl/IP), or 5 μl/IP anti-GFP (A-11122 ...
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bioRxiv - Immunology 2024Quote: ... dried immunoprecipitates were reconstituted in buffer (1% acetonitrile,0.1% TFA) and applied to a 10cm RP-18e 100-4.6 chromolith column (Merck) using an Ultimate 3000 HPLC equipped with UV monitor.
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bioRxiv - Pathology 2024Quote: ... after a 2 hours fasting period followed by an intraperitoneal (i.p.) injection of human insulin (0.75 U.kg-1 of body weight; I9278, Merck, Germany), glycaemia was measured in the same way as for the OGTT.
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bioRxiv - Biochemistry 2024Quote: ... Reactions were later visualized by Coomassie staining and western blotting with anti-ubiquitin (Merck,#05-944, 1:5000) antibody or anti-His (GeneScript ...
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bioRxiv - Biochemistry 2024Quote: ... PD elutions were visualized by Coomassie staining and western blotting with anti-ubiquitin (Merck, #05-944, 1:5000) antibody after separation on an SDS-PAGE gel.
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bioRxiv - Biochemistry 2024Quote: ... Each sample solution or marker (1 μl) was spotted onto a TLC plate (silica gel 60 F254, Merck). The TLC plates were developed appropriate times with 75% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... were homogenised in 250 mM sucrose / 20 mM triethanolamine with protease inhibitors (1% Merck Protease Inhibitor Cocktail III). The homogenate was cleared of large debris by centrifugation at 4000 g for 15 mins at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blot was performed according to standard procedures using the following antibodies: NELFE (1:500, Merck-Millipore, ABE48), Top1 (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... and then repeatedly washed with PBS-T before incubation in secondary antibodies for GRIN2D (Merck, MAB5578, 1:500) and B-actin (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with an Alexa Fluor 568 donkey anti-rabbit secondary antibody (1/500, Merck, France, #A10042) for 90 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... RSV F-protein was detected using a mouse anti-RSV antibody used 1:5000 in casein (MAB8262, Merck), which was incubated at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were analyzed by Coomassie staining and western blotting with anti-ubiquitin antibody (Merck, #05-944, 1:5000) after separation on a 4-15% gradient SDS-PAGE gel.
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bioRxiv - Cancer Biology 2024Quote: ... Chromatography was performed using Sequant ZIC-HILIC column (1 mm x 150 mm, 3.5 μm, 200 Å, Merck) on an Agilent 1260 Infinity HPLC at 50 μL/min flowrate in normal flow mode ...
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bioRxiv - Developmental Biology 2024Quote: ... used at 1:1000) were also used as fluorescent counter stains and mounted with coverslips using Fluorsave (Merck). Imaging was performed on Zeiss LSM-800 confocal microscopy and images managed using Zen Black software (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Minced testes were dissociated in Dulbecco’s modified Eagle medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 1 mg/ml collagenase I (Merck) for 25 min at 37 °C and 0.05% trypsin-EDTA (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed at room temperature for 1 hour followed by several washing steps and subsequent embedding with Mowiol optionally containing DAPI (#1.24653; Merck).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... cell culture supernatant of HUVECs was concentrated to 1 mL using Amicon-Ultra 15 Centrifugal Filter Units (UFC901096, Merck) and incubated with 0.5 µg of Alexa 488 labeled anti-CD9 ...
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bioRxiv - Molecular Biology 2021Quote: ... The enzymes were concentrated to 10-19.5 mg ml−1 with Amicon Ultra 10k and 30k MWCO centrifugal filters (Merck), flash frozen in liquid nitrogen and stored directly at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 6 times for 10min with 1ml wash buffer (10mM HEPES ph 7.3, 150mM NaCl, MgCl2, 10% glycerol, 1% Empigen BB Detergent (Merck), 1x PI) ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal (RP) against phosphorylated Tyr 1077 ObRb (pTyr1077ObR; 1:500, cat# 07-1317, Merck Millipore, Burlington, Vermont, USA), GP against phosphorylated Tyr 1138 ObRb (pTyr1138ObRb ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We next centrifuged the mix (1 min, 13,000 g) through a Durapore PVDF 0.22-μm filter (Merck Millipore, US). We injected the CRISPR-Cas9 solution into the male pronucleus of fertilized zygotes with a motor-driven manipulator-based microinjection stage ...
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bioRxiv - Cell Biology 2021Quote: ... the nuclear pellet was resuspended in 0.5 mL of ice-cold RIPA buffer supplied with 1 U/mL Benzonase (Merck), and incubated on a rotator for 1 h at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Myosin II filaments were further diluted in KMEH to 200 nM and added to the actin filaments bound to the SLB by His6-Curly by replacing 1/10 of the sample buffer with the myosin II filament solution and supplemented with 0.1 mM Mg-ATP as well as a mix of 1 mM Trolox (cat. no. 648471, Merck), 2 mM protocatechuic acid (cat ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 1x 106 cells of each cell line were spin-infected for 1 h at 1.9 × 103 g (3000 rpm) in the presence of 10 μg/mL polybrene (Merck) to facilitate the infection ...
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Microbiology 2021Quote: ... which were fixed with methanol for 10 seconds and then stained with Giemsa (1:10 dilution in water; Merck) for 5-10 min prior to microscopy under 100 x magnification with oil.
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 60 minutes followed by proteinase K solution treatment (7.5 μg ml−1 proteinase K; Merck, Darmstadt ...
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bioRxiv - Cell Biology 2021Quote: ... brain sections were subjected to immunohistochemistry using a mouse anti-NeuN antibody (MAB377; Merck, Kenilworth, NJ, USA; 1:500) followed by incubation with an anti-mouse IgG antibody (Vector Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then trypsinized and placed in cell culture inserts (1 × 106 cells per insert) with PTFE membrane (Merck) in 6 well plates for overnight cultivation ...
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bioRxiv - Microbiology 2022Quote: ... two ml of bacterial suspension (IPO 2222 WT or selected □D5-resistant IPO 2222 Tn5 mutants) adjusted to 106 CFU mL-1 in sterile Ringer’s buffer (Merck) was sprayed using a manual sprayer on the adaxial surface of each detached leaf ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... anti-A17L (1:500) in combination with horseradish peroxidase (HRP)-coupled secondary antibodies (1:2,000) and Immoblion Forte Western HRP substrate (Merck) or with IR-Dye secondary antibodies (Licor ...
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bioRxiv - Immunology 2021Quote: ... Samples were postfixed with 0.1 M sodium cacodylate buffer supplemented with 1 % osmium tetroxide (Science Services) and potassium ferrocyanide (Merck) and embedded in 1 % agarose (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: Type 1 diabetes was induced with a single intraperitoneal (i.p.) injection of 150 mg/kg streptozotocin (STZ; Merck, France) freshly dissolved in 0,9 % NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were then dried and stained on a heating block with a 1% toluidine blue (Merck, 89640-5G) solution including 2% Borax for 90 s at 60°C ...
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bioRxiv - Bioengineering 2020Quote: ... labeled for 30 minutes at 4ºC and washed with PBA (PBS with 0.2% sodium azide [Merck]; 1% BSA [Sigma]).DAPI (final concentration of 1 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Pathology 2021Quote: ... 96-well plate was coated with 1 μg/ml monoclonal anti-lubricin antibody clone 9G3 (Merck Millipore, Billerica, MA) in TBS at 4°C over-night ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-actin monoclonal antibody (#MAB1501R, used at 1:5,000 dilution for the Western blot analyses, Merck Inc.); a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged at 70 – 90% confluency using 1% PBS and trypsin/EDTA (0.25%,0.02%) in T75 tissue culture flasks (Merck, Australia) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...